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2024: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2024)

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Antioxidant, Medicinal Plants, and Antibacterial Microalgae, Plankton, Feed, Anti-Biofilm, and Nanoparticles

Purification of Phycocyanin from Spirulina platensis Using Natural Deep Eutectic Solvents with Varied Hydrogen Bond Donor

https://doi.org/10.20473/jipk.vi.54788
Eirene Tentua
IPB University
https://orcid.org/0000-0001-6193-3830
Safrina Dyah Hardiningtyas
IPB University
https://orcid.org/0000-0002-6514-1248
Iriani Setyaningsih
IPB University
https://orcid.org/0000-0001-6771-2748

Corresponding Author(s) : Eirene Tentua

eirene.tentua@gmail.com

Jurnal Ilmiah Perikanan dan Kelautan, 2024: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2024)

Abstract

Graphical Abstract 



Highlight Research



  1. Phycocyanin extract from Spirulina platensis was achieved.

  2. Highly pure phycocyanin was obtained through NaDES purification method.

  3. Strong antioxidant activity of phycocyanin was observed (IC50 <50 ppm).

  4. Successful removal of impurities confirmed by gel electrophoresis.


Abstract


Phycocyanin, a pharmacologically potent blue pigment extracted from Spirulina platensis, requires optimal purity for effective utilization. Traditional purification methods, although effective, are impeded by time and cost constraints. Addressing this challenge, aqueous two-phase systems (ATPs) incorporating natural deep eutectic solvents (NaDES) emerge as promising alternatives. These systems offer operational simplicity and cost-effectiveness, ensuring efficient purification with high purity and optimal recovery of phycocyanin. This study investigated the impact of various types of hydrogen bond donors (HBD) within choline chloride-based natural deep eutectic solvents (NaDES) on the purification of phycocyanin. Phycocyanin was extracted from Spirulina platensis biomass through ultrasonication and purified using an aqueous two-phase system with various HBD of NaDES, including urea, glycerol, and citric acid. The experimental design adhered to a completely randomized design. The initial purity index and yield of crude extract phycocyanin were 0.54±0.02 and 12.79±0.27%, respectively. The NaDES system with ChCl:citric acid exhibited superior performance, demonstrating a high purity index and recovery (2.3-fold, 71.83±2.36%) compared to ammonium sulfate (1.5-fold, 70.15±4.10%). The obtained phycocyanin was partially pure compared to commercial phycocyanin (purity index: 1.60), as indicated by SDS-PAGE. Moreover, the antioxidant activity of phycocyanin was enhanced post-purification, evident in the IC50 value of 40.54 ppm. In summary, organic acid-based NaDES has proven effective in increasing the purity and achieving a significant recovery percentage of phycocyanin compared to conventional ammonium sulfate methods. The antioxidant activity of phycocyanin was enhanced after purification. These results indicate the promising potential of NaDES-based ATP systems for producing functional protein-based ingredients, exemplified by phycocyanin.

Keywords

aqueous two-phasecitric aciddeep eutectic solventsphycobiliproteinultrasonication
Tentua, E., Hardiningtyas, S. D. ., & Setyaningsih, I. . (2024). Purification of Phycocyanin from Spirulina platensis Using Natural Deep Eutectic Solvents with Varied Hydrogen Bond Donor . Jurnal Ilmiah Perikanan Dan Kelautan. https://doi.org/10.20473/jipk.vi.54788
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