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2025: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2025)

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Aquaculture

The Characteristics of Chitosan Derived from Lobster Shells and its Effect on Fungi Activity and Water Stability of Lobster Pellets

Muhsinul Ihsan
Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri Mataram
https://orcid.org/0000-0002-3776-8748
Ali Harris
Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri Mataram
https://orcid.org/0000-0002-1980-2236
Mukminah
Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri Mataram
https://orcid.org/0009-0004-6113-2290
Megawati
Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri Mataram
https://orcid.org/0000-0003-2604-3335
Nining Purwati
Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri Mataram
https://orcid.org/0000-0002-8132-1011
Handa Muliasari
Department of Pharmacy, Faculty of Medicine, Universitas Mataram, Indonesia
https://orcid.org/0000-0002-4214-9124
Bayu Priyambodo
Lombok Marine aquaculture Development Centre, Indonesia.
https://orcid.org/0000-0002-3487-2179
Clive Jones
Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Australia
https://orcid.org/0000-0002-6319-2068
Leo Nankervis
Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Australia
https://orcid.org/0000-0001-6845-690X

Corresponding Author(s) : Muhsinul Ihsan

muhsinulihsan@gmail.com

Jurnal Ilmiah Perikanan dan Kelautan, 2025: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2025)

Abstract

Graphical Abstract



 


Highlights of Research



  1. The chitosan was successfully produced from lobster shells Panulirus homarus.

  2. The characteristics and quality of chitosan from lobster shells Panulirus homarus were observed.

  3. The anti-fungal activity of chitosan was assessed.

  4. Chitosan enhances the water stability of lobster pellets.


 


 


Abstract


Tropical rock lobster aquaculture is a lucrative industry that is currently limited by the lack of appropriate formulated feed. Its nocturnal, benthic feeding behavior necessitates a water-stable feed that maintains integrity under tropical marine conditions without degrading. Chitosan is a carbohydrate derived from the chitin of arthropod exoskeletons and mollusk shells, with documented applications as an anti-fungal agent as well as production of edible films and coatings. Chitosan therefore warrants research attention as a functional binder for tropical lobster feeds. We report on the characteristics of chitosan extracted from the exoskeleton of spiny lobsters (Panulirus homarus), including its effect on fungal activity and water stability of pellets. Chitosan was produced through three main steps: deproteination, demineralization, and deacetylation. The resulting chitosan was characterized through crude composition (AOAC methods), FTIR spectra, and scanning electron microscope (SEM), while anti-fungal activity was assessed through in vitro assays. Chitosan was used to coat lobster feed pellets by immersion method at different concentrations (0%, 0,5%, 1%, 1,5%, and 2%), and its impact on pellet water stability was assessed. There were three replications in fungal activity and water stability test. The yield of chitosan was 5.9 ± 0.01% of the total shell mass, with 96.99% ± 0.01 degree of deacetylation (DD). The resulting product contained 5.94 ± 0.07% moisture, 36.72 ± 0.05% ash and 2.73 ± 0.08% nitrogen. Chitosan morphology was characterized as an irregular shape with dimensions ranging from 157 to 391 µm, with a combination of striated surface textures. Increasing concentration of chitosan increased water stability of pellets up to 1.5% inclusion, while 0.5% optimized Fusarium sp, inhibition. 

Keywords

Chitosanlobsteranti-fungalcoatingpellets
Ihsan, M., Harris, A., Mukminah, Megawati, Purwati, N., Muliasari, H., Priyambodo, B., Jones, C., & Nankervis, L. (2025). The Characteristics of Chitosan Derived from Lobster Shells and its Effect on Fungi Activity and Water Stability of Lobster Pellets. Jurnal Ilmiah Perikanan Dan Kelautan. Retrieved from https://e-journal.unair.ac.id/JIPK/article/view/57474
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Author Biographies

Muhsinul Ihsan, Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri Mataram

Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri
Mataram

Ali Harris, Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri Mataram

Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri
Mataram

Mukminah, Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri Mataram

Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri
Mataram

Megawati, Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri Mataram

 Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri
Mataram

Nining Purwati, Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri Mataram

Department of Biology Education Faculty of Teacher and Treaning Universitas Islam Negeri
Mataram

Handa Muliasari, Department of Pharmacy, Faculty of Medicine, Universitas Mataram, Indonesia

Department of Pharmacy, Faculty of Medicine, Universitas Mataram, Indonesia

Bayu Priyambodo, Lombok Marine aquaculture Development Centre, Indonesia.

 Lombok Marine Aquaculture Development Centre, Indonesia

Clive Jones, Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Australia

Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Australia

Leo Nankervis, Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Australia

Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Australia

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