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Vol. 17 No. 2 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Aquaculture Fisheries Biotechnology

Environmental Effect on the Growth and Enzyme Activity of Fucoidanase-Producing Bacteria Cytobacillus kochii GSD

https://doi.org/10.20473/jipk.v17i2.69263
Agus Setyawan
Department of Aquaculture, Faculty of Agriculture, University of Lampung, Bandar Lampung. Indonesia
https://orcid.org/0000-0003-2728-1159
Muhammad Kholiqul Amiin
Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.
https://orcid.org/0000-0003-1585-1046
Hilma Putri Fidyandini
Department of Aquaculture, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.
https://orcid.org/0000-0001-8467-6400
Almira Fardani Lahay
Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.
https://orcid.org/0000-0002-5477-3143
Maulid Wahid Yusup
Department of Aquaculture, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.
https://orcid.org/0009-0007-9437-0473
Alifia Adibila Nurhalisa
Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.
https://orcid.org/0009-0003-0409-6492
Rizqy Hadi Saputra
Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.
https://orcid.org/0009-0006-7715-9863
Wanda Andella Putri
Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.
https://orcid.org/0009-0004-6759-5317
Ni Luh Gede Ratna Juliasih
Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Lampung, Bandar Lampung, Indonesia
https://orcid.org/0000-0002-6202-461X
Arief Rahman Rivaie
Research Center for Fishery, National Research and Innovation Agency (BRIN), Cibinong, Indonesia.
https://orcid.org/0000-0001-8755-8944
Suryadi Saputra
Research Center for Fishery, National Research and Innovation Agency (BRIN), Cibinong, Indonesia.
https://orcid.org/0000-0001-5071-4878

Corresponding Author(s) : Agus Setyawan

agus.setyawan@fp.unila.ac.id

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 17 No. 2 (2025): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Graphical Abstract



 


Highlight Research


1. Sargassum polycystum has been extracted and analyzed.


2. Cytobacillus kochii GSD in response to fucoidanase-producing enzyme activity was analyzed.


3. Optimum temperature, pH, and salinity can suppress the activity of the fucoidanase enzyme through the inhibition test.


4. Cytobacillus kochii GSD can produce fucoidanase enzyme with low molecular weight.


 


 


Abstract


Extensive research has shown that low molecular weight fucoidan exhibits significantly greater biological activity than its high molecular weight. C. kochi GSD, a Sargassum symbiont bacterium, is proven to have the activity of hydrolyze fucoidan. This study proposes the growth optimization and fucoidanase enzymatic activity of C. kochii GSD bacteria under varying environmental conditions (temperature, pH, and salinity) cultured in basic liquid medium (BLM) for 48 hours. Based on Response Surface Methodology (RSM), the range of temperature, pH, and salinity for the growth optimization test of C. kochi GSD bacteria were 26.591, 30, 35, 40, and 43.49oC, the pH used starts from 3.636, 5, 7, 9, and 10.363, while the salinity to be used starts from 3.522, 5, 17.5, 30, and 38.522 ppt, respectively. The best conditions for growth of each environment were then continued with the test of fucoidanase enzyme activity in vitro. The results showed that C. kochii GSD bacteria grew optimally at temperature, pH, and salinity of 35oC, 7, and 30 ppt, respectively. The optimum enzyme activity of C. kochii GSD is at 72 hours with the forming of clear zones on media containing fucoidan and given Cetylpyridinium chloride (CPC) solution with clear zone diameters of 31.17 mm (temperature), 25.9 mm (pH), and 20.97 mm (salinity), respectively. The conclusion of this study is a high novelty finding to produce low molecular weight fucoidan enzymatically with C. kochii GSD bacteria to be used as an immunostimulant.

Keywords

FucoidanaseImmunostimulantlow molecular weightOptimization bacterial growth
Setyawan, A., Amiin, M. K., Fidyandini, H. P., Lahay, A. F., Yusup, M. W., Nurhalisa, A. A., Saputra, R. H., Putri, W. A., Ratna Juliasih, N. L. G. ., Rivaie, A. R., & Saputra, S. (2025). Environmental Effect on the Growth and Enzyme Activity of Fucoidanase-Producing Bacteria Cytobacillus kochii GSD. Jurnal Ilmiah Perikanan Dan Kelautan, 17(2), 438–452. https://doi.org/10.20473/jipk.v17i2.69263
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Author Biographies

Agus Setyawan, Department of Aquaculture, Faculty of Agriculture, University of Lampung, Bandar Lampung. Indonesia

Department of Aquaculture, Faculty of Agriculture, University of Lampung, Bandar Lampung. Indonesia

Muhammad Kholiqul Amiin, Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Hilma Putri Fidyandini, Department of Aquaculture, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Department of Aquaculture, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Almira Fardani Lahay, Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Maulid Wahid Yusup, Department of Aquaculture, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Department of Aquaculture, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Alifia Adibila Nurhalisa, Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Rizqy Hadi Saputra, Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Wanda Andella Putri, Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Department of Marine Science, Faculty of Agriculture, Universitas Lampung, Bandar Lampung City, Lampung, Indonesia.

Ni Luh Gede Ratna Juliasih, Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Lampung, Bandar Lampung, Indonesia

Department of Chemistry, Faculty of Mathematics and Natural Science, Universitas Lampung, Bandar Lampung, Indonesia

Arief Rahman Rivaie, Research Center for Fishery, National Research and Innovation Agency (BRIN), Cibinong, Indonesia.

Research Center for Fishery, National Research and Innovation Agency (BRIN), Cibinong, Indonesia.

Suryadi Saputra, Research Center for Fishery, National Research and Innovation Agency (BRIN), Cibinong, Indonesia.

Research Center for Fishery, National Research and Innovation Agency (BRIN), Cibinong, Indonesia.

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