The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi
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The aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used disk diffusion method. Oxytetracycline 30 µg (Oxoid CT0041B) was used as the Positive Control (K+). CMC Na 0,5% was used as the Negative Control (K-). The treatment (P1, P2, P3, P4) used various concentrations of gamal leaves ethanol extract, which is 40%, 50%, 60%, and 70%. The study assigned six treatments and four replication. The obtained data were analyzed by ANOVA, then continued with the DUNCAN test if the results showed significant differences (p<0,05). The inhibition result of positive control (K+) was 24,70c ± 7.04; and the negative control was 0,00a ± 0,00. All extract concentration result different inhibition, concentration 1 (P1) was 10,41 b ± 3,10; concentration 2 (P2) 8,14 b ± 0,45; concentration 3 (P3) 8,08 b ± 0,47; and concentration 4 (P4) 8,01b ± 0,29. These results indicated that the gamal leaves ethanol extract has antibacterial activity to inhibit the growth of E. coli isolated from Olive Ridley semi-natural nest in Boom beach, Banyuwangi.
Abdulaziz, A. A., Dapar, M. L. G., Manting, M. M. E., Torres, A. J., Aranas, A. T., Mindo, R. A. R., & Demayo, C. G. (2019). Qualitative evaluation of the antimicrobial, antioxidant, and medicinally important phytochemical constituents of the ethanolic extracts of the leaves of Gliricidia sepium (Jacq.). Pharmacophore, 10(4), 72-83.
Al-Bahry, S. N., Mahmoud, I., Elshafie, A., Al-Harthy, A., Al-Ghafri, S., Al-Amri, I., & Alkindi, A. (2011). Bacterial Flora and Antibiotic Resistance from Eggs of Green Turtles (Chelonia mydas) An Indication of Polluted Effluents. Marine Pollution Bulletin, 58, 720-725.
Alfaro, A., Køie, M., & Buchmann, K. (2006). Synopsis of Infections in Sea Turtles Caused by Virus, Bacteria and Parasites: an Ecological Review. University the Copenhagen, p.30.
Anwar, S., Febria, F. A., & Nasir, N. (2014). Identifikasi Koleksi Jamur dari Cangkang dan Pasir Sarang Telur Penyu Lekang (Lepidochelys olivacea L.) di Penangkaran Pariaman. Jurnal Biologi UNAND, 3(1).
Artaningsih, N. L. B., Habibah, N., & Nyoman, M. (2018). Aktivitas Antibakteri Ekstrak Etanol Daun Gamal (Gliricidia sepium) pada Berbagai Konsentrasi terhadap Pertumbuhan Bakteri Streptococcus mutans secara In-Vitro. Jurnal Kesehatan, 9(3), 336-345.
Azaria, D. (2014). Perlindungan Lingkungan Laut Samudra Pasifik dari Gugusan Sampah Plastik Berdasarkan Hukum Lingkungan Internasional (Doctoral dissertation, Universitas Brawijaya).
Cappucino, J. G., & Sherman, N. (1992). Microbiology, A Laboratory Manual. The Benyamins/Cummings Publishing Co., Inc., New York.
Elfidasari, D., Toufan, G., & Irawan, S. (2017). Deteksi Cemaran Mikroorganisme pada Kawasan Konservasi Penyu di Pangumbahan Sukabumi. Al-Azhar Indonesia Seri Sains dan Teknologi, 4(1), 28.
Handajani, N. S., & Purwoko, T. (2008). Aktivitas Ekstrak Rimpang Lengkuas (Alpinia galangal) Terhadap Pertumbuhan Jamur Aspergillus sp. Penghasil Aflatoksin dan Fusarium moniliforme. Biodiversitas, 9(3), 161-4.
Haprabu, B. R. S. (2018). Isolasi dan Identifikasi Cemaran Bakteri Escherichia coli pada Telur Penyu Lekang yang Gagal Menetas di Sarang Semi Alami Pantai Boom Banyuwangi [Skripsi]. Fakultas Kedokteran Hewan. Universitas Airlangga.
Hau, E. E. R., & Rohyati, E. (2017). Aktivitas Antibakteri Nira Lontar Terfermentesi Dengan Variasi Lama Waktu Fermentasi Terhadap Bakteri Gram Positif (Staphylococcus Aureus) Dan Gram Negatif (Escherichia Coli). Jurnal Kajian Veteriner, 5(2), 91-98.
Hidayat, O., Febria, F. A., & Nasir, N. (2014). Isolasi dan Karakterisasi Bakteri pada Pasir Sarang dan Cangkang Telur Penyu lekang (Lepidochelys olivaceae) yang Menetas dan Gagal Menetas. Jurnal Biologi UNAND, 3(2).
Jawetz, E., Melnick, J., & Adelberg. (2004). Mikrobiologi Kedokteran Edisi 23. EGC. Jakarta.
Karlina, C. Y., Ibrahim, M., & Trimulyono, G. (2013). Aktivitas Antibakteri Ekstrak Herba Krokot (Potulaca oleracea L.) Terhadap Staphylococcus aureus dan Escherichia coli. Lentera Bio, 2(1), 87-93.
Keene, E. L. (2012). Microorganism From Sand Cloacal Fluid and Eggs of Lepidochelys olivacea and Standart Testing of Cloacal Fluid Antimircobial Properties [Thesis]. Faculty of Purdue University
Madduliri, S., Rao, K. B., & Sitaram, B. (2013). In Vitro Evaluation of Five Indegenous Plants Extract Against Five Bacterial Phatogens of Humans. International Journal of Pharmacy and Pharmaceutical Science, 5(4), 679-684.
Messens, W., Grijspeerdt, K., & Herman, L. (2005). Eggshell Characteristics and Penetration by Salmonella Enterica Serovar Enteritidis Through the Production Period of a Layer Flock. British Poultry Science, 46(6), 694-700.
Monalisa, D. T., Handayani, & Sukmawati, D. (2011). Uji Daya Antibakteri Ekstrak Daun Tapak Liman (Elephantopus sacber L.) Terhadap Staphylococcus aureus dan Salmonella typhi. Jurnal Biomia, 9(2), 13-20.
Musdalifah, M., Khumaidi, A., & Suwastika, I. N. (2017). Uji Daya Hambat dan Skrining Fitokimia Ekstrak Daun Macaranga tanarius (L.) Mull. Arg Sebagai Antibakteri Salmonella typhi. Natural Science: Journal of Science and Technology, 6(3).
Nuria, M. C., & Faizatun, A. (2009). Uji Aktivitas Antibakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha Curcas L) Terhadap Bakteri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, dan Salmonella typhi ATCC 1408. Mediagro, 5(2).
Pangalinan, F., Kojong, N., & Yamlean, P. (2012). Uji Aktivitas Antijamur Ekstrak Etanol Kulit Rambutan (Nephelium lappaceum L.) terhadap Jamur Candida albicans Secara In Vitro. Pharmacon, 1(1), 7- 12.
Permana, I. (2016). Desain Konseptual Marina Dengan Theory Of Constraint: Studi Kasus Pantai Boom Kabupaten Banyuwangi (Doctoral dissertation, Institut Teknologi Sepuluh Nopember).
Plumb, Donald, C. (2009). Veterinary Drug Handbook 7th Edition. Pharmavet. Stockholm, Wisconsin.
Poeloengan, M., & Praptiwi, P. (2010). Uji aktivitas antibakteri ekstrak kulit buah manggis (Garcinia mangostana Linn). Media Penelitian dan Pengembangan Kesehatan, 20(2).
Praja, R. N., Yudhana, A., & Haditanojo, W. (2018). Isolasi dan Identifikasi Jamur pada Cangkang Telur Penyu Lekang (Lepidochelys olivacea) Gagal Menetas di Pantai Boom Banyuwangi. Jurnal Medik Veteriner, 1(2), 43-47.
Prameswari, R. A. (2019). Deteksi Residu Antibiotik Oksitetrasiklin Pada Susu Kambing Peranakan Etawa Di Kelurahan Kalipuro Kecamatan Kalipuro Banyuwangi Dengan Metode Uji Bioassay. Jurnal Medik Veteriner, 2(2), 112.
Pratama, G., Kurniawan, I. D., & Ilhamdy, A. F. (2020). Pengendalian Pencemaran Limbah Domestik sebagai Upaya Rehabilitasi Pesisir di Desa Malangrapat, Kabupaten Bintan. PRIMA: Journal of Community Empowering and Services, 4(1), 45-50.
Rock, C., & Rivera, B. (2014). Water quality, E. coli and your health. The University of Arizona-College of Agriculture and Life Sciences-Cooperative Extension.
Sarudji, S., Chusniati, S., Tyaningsih, W., & Handijanto, D. (2017). Petunjuk Praktikum Penyakit Infeksius I Program S-1 Kedokteran Hewan, Departemen Mikrobiologi Fakultas Kedokteran Hewan Universitas Airlangga. Hal: 32-37.
Siqueira, J. F., & Rocas, I. N. (2011). Microbiology and Treatment of Endodontic Infections. In Cohen’s Pathways of The Pulp. 10th eds. Hargreaves KM, Cohen S. China: Elsevier. pp: 565-84.
Sukamto, T., Muryanto, & Sarbini, R. (2016). Teknik Penetasan Telur Penyu Hijau (Chelonia mydas) di Kawasan Konservasi, Pantai Pangumbahan, Kabupaten Sukabumi, Jawa Barat. Buletin Teknik Litkayasa (BTL) Sumber Daya dan Penangkapan, 14(1), 29-32.
Tedju, J. B., Bukit, M., & Johannes, A. Z. (2018). Kajian Awal Sifat Optik Senyawa Hasil Ekstraksi Daun Gamal (Gliricidia Sepium) Asal Kota Kupang. Jurnal Fisika: Fisika Sains dan Aplikasinya, 3(2), 142-146.
Tjay, T. H., & Rahardja, K. (2007). Obat-obat Penting Khasiat, Penggunaan dan Efek-efek Sampingnya. Edisi ke IV. PT Elex Media Komputindo. Jakarta. Hal: 19, 58.
Tula, M. Y., Azih, A. V., Iruojale, F. O., Okojie, R. O., Elimian, K. O., & Toy, B. D. (2012). Systematic Study on Comparing Phytochemicals and The Antimicrobial Activities from Different Parts of V. Amygdalina. African Journal of Microbiology Res, 6(43), 7089-7093.
Wicaksono, M. A., Nurhasanah, F., Elfidasari, D., & Sugoro, I. (2017). Cemaran Mikroba Pada Telur Penyu Sisik (Eretmochelys imbricata) di Pulau Kelapa Dua, Taman Nasional Laut Kepulauan Seribu, DKI Jakarta. Jurnal Al-azhar Indonesia Seri Sains dan Teknologi, 4(2), 83-90.
Wulandari, C., Nasir, N., & Agustien, A. (2014). Kondisi bakteriologis air sumur di sekitar tempat pembuangan akhir air dingin kota Padang. Jurnal Biologi UNAND, 3(4).
Zeniusa, P., Ramadhian, M. R., Nasution, S. H., & Karima, N. (2019). Uji Daya Hambat Ekstrak Etanol Teh Hijau TerhadapEscherichia coli Secara In Vitro. Jurnal Majority, 8(2), 136-143.
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