Comparison of different poultry egg yolks-citrate extender with green tea (Camellia sinensis) extract addition on Sapudi ram spermatozoa quality in chilled temperature storage
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Preservation of ram semen has an impact on the exposure of artificial conditions to the spermatozoa which was followed by overproduction of reactive oxygen species (ROS) and resulted in functional damages of spermatozoa. This study aimed to determine the effect of the combination of chicken, quail, and duck egg yolks with green tea extract (GTE) on the quality of Sapudi ram spermatozoa at 5°C storage. Ejaculates of Sapudi ram with more than 70% spermatozoa motility were divided into four groups. Semen were extended 1 : 10 in citrated-chicken egg yolk extender (control group, T0), and citrated yolk of chicken (T1), quail (T2), and duck (T3) egg added with 0.05 mg GTE per 100 mL of extender. Extended semen was kept in a tube, which was put in a beaker containing distilled water and stored in a refrigerator (5°C). Spermatozoa motility, viability, and plasma membrane integrity (PMI) were evaluated daily for five days. The results showed that GTE in duck egg yolk citrate extender could maintain the highest spermatozoa motility, viability, and PMI for five days (p <0.05). It could be concluded that the duck egg yolk citrate extender with the addition of GTE (Camellia sinensis) was beneficial for maintaining the semen of Sapudi ram spermatozoa at chilled temperatures. Further study is needed to use the extender for freezing Sapudi ram semen.
Al-Bulushi S, Manjunatha BM, Bathgate R, Rickard JP, de Graaf SP. 2019. Artificial insemination with fresh, liquid stored and frozen thawed semen in dromedary camels. PLoS One. 14: e0224992.
Anand M, Baghel G, Yadav S. 2017. Effect of egg yolk concentration and washing on sperm quality following cryopreservation in Barbari buck semen. J Appl Anim Res. 45: 560-5.
Aziz Z, Cyriac S, Beena V, Philomina PT. 2012. Comparison of cholesterol content in chicken, duck and quail eggs. J Vet Anim Sci. 43: 64-6.
Behera S, Harshan HM, Bhai KL, Ghosh KN. 2015. Effect of cholesterol supplementation on cryosurvival of goat spermatozoa. Vet World. 8: 1386-91.
Chakraborty S, Saha S. 2022. Understanding sperm motility mechanisms and the implication of sperm surface molecules in promoting motility. Middle East Fertil Soc J. 27: 4.
El-Shamaa I, El-Seify ES, Hussein A, El-Sherbieny M, El-Sharawy M. 2012. A Comparison of duck and chicken egg yolk for cryopreservation of Egyptian buffalo bull spermatozoa. Anim Sci. 55: 109-13.
Kameni S, Meutchieye F, Ngoula F. 2021. Liquid Storage of Ram Semen: Associated Damages and Improvement. Open J Anim Sci. 11: 473-500.
Khoirunnisa I, Susilowati S, Maslachah L, Suprayogi TW, Kurnijasanti R, Ratnani H. 2019. The effect of the addition of green tea (Camellia sinensis) extract in egg yolk citrate extender on the quality of sapudi ram spermatozoa stored at chilled temperature. Ovozoa 8: 127-31.
Kusmiyati M, Sudaryat Y, Lutfiah IA, Rustamsyah A, Rohdiana D. 2015. Aktivitas antioksidan, kadar fenol total, dan flavonoid total dalam teh hijau (Camellia sinensis (L.) O. Kuntze) asal tiga perkebunan Jawa Barat. J Penelitian Teh dan Kina 18: 101-6.
Liu Y, Qiu N, Gao D, Ma M. 2018. Comparative proteomic analysis of chicken, duck, and quail egg yolks. Int J Food Prop. 21: 1311-21.
Musial C, Kuban-Jankowska A, Gorska-Ponikowska M. 2020. Beneficial Properties of Green Tea Catechins. Int J Mol Sci. 21: 1744.
Oliveira LZ, Monteiro FM, de Arruda RP, Celeghini ECC. 2013. The importance of semen quality in AI programs and advances in laboratory analyses for semen characteristics assessment. In: Lemma A (Ed). Success in Artificial Insemination - Quality of Semen and Diagnostics Employed [Internet]. London. IntechOpen.
OndÅ™ej Å , JiÅ™í Å , Jan B, Pavla MP, Lucie T, Doležalová M, Petra F, LudÄ›k S, Radko R. 2019. Low Density Lipoprotein - important player in increasing cryoprotective efficiency of soybean lecithin-based bull semen extenders. Anim Reprod. 16: 267-76.
Parkinson TJ, Jane M. Morrell JM. 2019. Artificial Insemination. In: Noakes DE, Parkinson TJ, England GCW (Eds). Veterinary Reproduction and Obstetrics. 10th Ed. Elsevier.
Peña FJ, Ortiz-Rodríguez JM, Gaitskell-Phillips GL, Gil MC, Ortega-Ferrusola C, Martín-Cano FE. 2021. An integrated overview on the regulation of sperm metabolism (glycolysis-Krebs cycle-oxidative phosphorylation). Anim Reprod Sci. 14: 106805.
Sabeti P, Pourmasumi S, Rahiminia T, Akyash F, Talebi AR. 2016. Etiologies of sperm oxidative stress. Int J Reprod Biomed. 14: 231-40.
Shan S, Xu F, Hirschfeld M, Brenig B. 2021. Sperm Lipid Markers of Male Fertility in Mammals. Int J Mol Sci. 22: 8767.
Suprayogi TW, Susilowati S. 2018. The effect of cattle seminal plasma crude protein on the cryopreservation of goat semen. Iran J Appl Anim Sci. 8: 641-6.
Susilowati S, Sardjito T, Mustofa I, Widodo OS, Kurnijasanti R. 2021. Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients. Anim Biosci. 34: 198-204.
Susilowati S, Sardjito T, Widodo OS, Kurnijasanti R, Wurlina W, Safitri E, Mustofa I. 2018. Effect of green tea extract supplementation in the semen extender on post-thaw sperm quality of Simmental bulls. Philipp J Vet Med. 55: 127-34.
Teja DNGS, Bebas W, Trilakasana IGNB. 2018. Pengencer kuning telur berbagai jenis unggas mampu mencegah abnormalitas dan kerusakan membran spermatozoa ayam Pelung. Indones Med Veterinus 7: 262-70.
Widiastuti WA, Bebas W, Trilaksana IGNB. 2018. Penggunaan berbagai kuning telur sebagai bahan pengencer terhadap motilitas dan daya hidup spermatozoa ayam Pelung. Indones Med Veterinus 7: 252-61.
Wurlina W, Hariadi M, Safitri E, Susilowati S, Meles DK. 2020. The effect of crude guava leaf tannins on motility, viability, and intact plasma membrane of stored spermatozoa of Etawa crossbred goats. Vet World. 13: 530-7.
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