Effect of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic cells and Leydig cells of gentamicin-induced rats
Downloads
This study aimed to determine the effect of the administration of ethanolic extract of Moringa oleifera leaves on the number of spermatogenic and Leydig cells of gentamicin-induced rats (Rattus norvegicus). This study used 25 white male rats divided randomly into five groups. The rats were injected with Gentamicin 5 mg/kg bw/day subcutaneously for 14 days, except for the negative control group (NCG). During the next 14 days, the rats were given oral moringa leaf extract of 200, 316, and 500 mg/kg bw/day respectively for E200, E316, and E500 groups. The data were analyzed with one-way ANOVA followed by Duncan's test. The results showed that the number of spermatogonia, spermatocytes, and spermatids in NCG was higher (p <0.05) than in the other groups. The number of spermatogonia, spermatocytes, and spermatids in the E316 group was higher (p <0.05) than in PCG. The number of spermatogonia in the E500 group was higher (p <0.05) than in the E316 group, but the number of spermatocytes and spermatids in the E316 group was similar (p >0.05) to the E500 group. The number of PCG Leydig cells was the smallest (p <0.05) compared to the other groups. The administration of Moringa leaves extract in the E200 and E316 groups increased (p <0.05) the number of Leydig cells compared to PCG. The number of Leydig PCG cells in the E500 group was not significantly different (p >0.05) from that of the NCG. It could be concluded that administration of Moringa oleifera leaves extract restored the number of spermatogenic and Leydig cells of gentamicin-induced rats.
Ajayi AF, Akhigbe RE. 2020. The physiology of male reproduction: Impact of drugs and their abuse on male fertility. Andrologia 52: e13672.
Aladamat N, Tadi P. 2022. Histology, Leydig Cells. In: StatPearls [Internet]. StatPearls Publishing; USA. Updated 2022 Nov 14.
Aly H. 2019. Testicular toxicity of gentamicin in adult rats: Ameliorative effect of lycopene. Hum Exp Toxicol. 38: 1302-13.
Asadi N, Bahmani M, Kheradmand A, Rafieian-Kopaei M. T2017. The impact of oxidative stress on testicular function and the role of antioxidants in improving it: A review. J Clin Diagn Res. 11: IE01-IE05.
Chaves BJ, Tadi P. 2023. Gentamicin. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing. USA. Updated 2023 Apr 10].
Gamaan MA, Zaky HS, Ahmed HI. 2023. Gentamicin-induced nephrotoxicity: A mechanistic approach. Azhar Int J Pharm Med Sci. 3: 11-9.
Gopalakrishnan L, Doriya K, Kumar DS. 2016. Moringa oleifera: A review on nutritive importance and its medicinal application. Food science and human wellness. 5: 49-56.
Grande G, Barrachina F, Soler-Ventura A, Jodar M, Mancini F, Marana R, Chiloiro S, Pontecorvi A, Oliva R, Milardi D. 2022. The role of testosterone in spermatogenesis: lessons from proteome profiling of human spermatozoa in testosterone deficiency. Front Endocrinol. 13: 852661.
Griswold MD. 2015. Spermatogenesis: The commitment to meiosis. Physiol Rev. 96: 1-17.
Gunawati LS, Berata IK, Setiasih NLE. 2019. Histopathological structure of Wistar rats testicle with excessive physical activities that given kelor leaf extract. Indon Med Vet. 8: 637-46.
Hasanin NA, Sayed NM, Ghoneim FM, Al-Sherief SA. 2018. Histological and ultrastructure study of testes of acrylamide exposed adult male albino rat and evaluation of the posible effect of vitamin E intake. J Mic Ultras. 6: 23-34.
Hegazi MAM, Elebshany IAK. 2019. Ameliorative effect of Moringa oleifera on oxidative stress in male albino rat brain promotes by aluminium exposure. Nat Sci. 17: 92-100.
Krause KM, Serio AW, Kane TR, Connolly LE. 2016. Aminoglycosides: An overview. Cold Spring Harb Perspect Med. 6: a027029.
Mardatillah M, Wurlina W, Yudaniayanti IS, Plumeriastuti H, Primarizky H, Hamid IS. 2022. Moringa oleiferaleaf extract restored the diameter and thickness of the seminiferous tubules of rat (Rattus norvegicus) injected with gentamicin. Ovozoa : J Anim Reprod. 11: 15-21.
Martemucci G, Costagliola C, Mariano M, D'andrea L, Napolitano P, D'Alessandro AG. 2022. Free radical properties, source and targets, antioxidant consumption and health. Oxygen 2: 48-78.
Mohlala K, Offor U, Monageng E, Takalani NB, Opuwari CS. 2023. Overview of the effects of Moringa oleifera leaf extract on oxidative stress and male infertility: A review. Appl Sci. 13: 4387.
Nedresky D, Singh G. 2023. Physiology, Luteinizing Hormone. In: StatPearls [Internet]. StatPearls Publishing. USA. Updated 2022 Sep 26.
Nishimura H, L'Hernault SW. 2017. Spermatogenesis. Curr Biol. 27: R988-R994.
Oduwole OO, Huhtaniemi IT, Misrahi M. 2021. The roles of Luteinizing Hormone, Follicle-Stimulating Hormone and Testosterone in spermatogenesis and folliculogenesis Revisited. Int J Mol Sci. 22: 12735.
Ogunsola OA, Joshua O, Sunday FO, Nwobi NL, Falugi B, Akinbola AS. 2017. Moringa plants parts consumption had effects on reproductive functions in male and female rat models. J Dent Med Sci. 16: 82-6.
Orlowski M, Sarao MS. 2023. Physiology, Follicle Stimulating Hormone. In: StatPearls [Internet]. StatPearls Publishing. USA. Updated 2023 May 1.
Panche AN, Diwan AD, Chandra SR. 2016. Flavonoids: an overview. J Nut Sci. 5: 1-15.
Peñalver R, Martínez-Zamora L, Lorenzo JM, Ros G, Nieto G. 2022. Nutritional and antioxidant properties of Moringa oleifera leaves in functional foods. Foods 11: 1107.
Ross JDC, Brittain C, Cole M, Dewsnap C, Harding J, Hepburn T, Jackson L, Keogh M, Lawrence T, Montgomery AA, Roberts TE, Sprange K, Tan W, Thandi S, White J, Wilson J, Duley L. 2019. G-ToG trial team. Gentamicin compared with ceftriaxone for the treatment of gonorrhoea (G-ToG): a randomised non-inferiority trial. Lancet 393: 2511-20.
Roy A, Khan A, Ahmad I, Alghamdi S, Rajab BS, Babalghith AO, Alshahrani MY, Islam S, Islam MR. 2022. Flavonoids a bioactive compound from medicinal plants and its therapeutic applications. Biomed Res Int. 2022: 5445291.
Shah W, Khan R, Shah B, Khan A, Dil S, Liu W, Wen J, Jiang X. 2021. The molecular mechanism of sex hormones on Sertoli cell development and proliferation. Front Endocrinol. 12: 648141.
Sikder K, Sinha M, Das N, KR Das DKR, Datta S, Dey S. 2013. Moringa oleifera leaf extract prevents in vitro oxidative DNA damage. Asian J Pharm Clin Res. 6: 159-63.
Sun J, Yin B, Tang S, Zhang X, Xu J, Bao E. 2019. Vitamin C mitigates heat damage by reducing oxidative stress, inducing HSP expression in TM4 Sertoli cells. Mol Reprod Dev. 86: 673-85.
Taha M, Elazab ST, Saati AA, Ahmed GS, Baokbah TAS, Fathy K, El-Shenbaby I, Abdelbagi O, Hassan MAE, Ibrahim MM, Badawy AM. 2022. Zamzam water ameliorates gentamicin-induced testicular toxicity in a rat model via targeting sperm parameters, testicular tissue oxidative insult, inflammation, apoptosis, and pituitary-gonadal axis. Toxics 11: 2.
Vergara-Jimenez M, Almatrafi MM, Fernandez ML. 2017. Bioactive components in Moringa oleifera leaves protect against chronic disease. antioxidants. 6:91.
Vona R, Pallotta L, Cappelletti M, Severi C, Matarrese P. 2021. The impact of oxidative stress in human pathology: Focus on gastrointestinal disorders. Antioxidants 10: 201.
Wang JM, Li ZF, Yang WX. 2022. What does androgen receptor signaling pathway in Sertoli cells during normal spermatogenesis tell us? Front Endocrinol (Lausanne) 13: 838858.
Ye RJ, Yang JM, Hai DM, Liu N, Ma L, Lan XB, Niu JG, Zheng P, Yu JQ. 2020. Interplay between male reproductive system dysfunction and the therapeutic effect of flavonoids. Fitoterapia. 147: 104756.
Zanaria R, Kamaluddin MT, Theodorus T. 2017. Efektivitas ekstrak etanol daun salam (Eugenia polyantha) terhadap GLTU di jaringan adiposa dan kadar gula darah puasa pada tikus putih jantan. J Biomed Fak Ked Univ Sriwijaya. 3: 145-52.
Zirkin BR, Papadopoulos V. 2018. Leydig cells: formation, function, and regulation. Biol Reprod. 99: 101-11.
Copyright (c) 2023 Nailul Ngizzah, Wurlina Wurlina, Poedji Hastutiek, Iwan Sahrial Hamid, Eka Pramyrtha Hestianah, Maslichah Mafruchati, Lita Rakhma Yustinasari
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Ovozoa by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under "the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.
