The effect of ciplukan (Physalis angulate Linn.) leaf extract on the testicles of rats (Rattus norvegicus) exposed to heat
Downloads
This study aims to determine the effect of administration of ciplukan (Physalis angulate Linn.) leaf extract on the variables of testicular weight, seminiferous tubules diameter, and spermatozoa plasma membrane integrity of rats (Rattus norvegicus) exposed to heat as model animals. Rats were divided randomly into five groups. In the NC group, rats were not exposed to heat and were only given 1% Na-CMC. In the PC, T1, T2, and T3 rats were exposed to heat followed by administration of 1% Na-CMC, 100, 200, and 400 mg/kg bw of ciplukan leaf extract (CLE) in 1% Na-CMC. Heat exposure at 40°C was conducted for 60 minutes every day for 21 days. On day-22, all rats were sacrificed for testicular evaluation. The results showed that all variables in the PC group were lower (p <0.05) than in the NC group. All variables in the T3 group were higher (p <0.05) than in the PC group. Testicular weight and spermatozoa plasma membrane integrity in the T3 group were not significantly different (p >0.05) from the NC group. However, the diameter of the seminiferous tubules in the T3 group were lower (p <0.05) than in the NC group. This study revealed that the administration of ciplukan leaf extract as an antioxidant increased testicular weight, seminiferous tubules diameter, and spermatozoa with intact plasma membrane in rats exposed to heat.
Aitken RJ, Roman SD. 2018. Antioxidant systems and oxidative stress in the testes. Oxid Med Cell Longev. 1:15-24.
Alam T, Ekayanti M, Permana N, Hadissabil Z. 2022. Potential Antioxidant Activity Of Ethanol Extract And Fraction Of Ciplukan (Physalis angulata) On DPPH (1,1-diphenyl-2-picrylhydrazyl). Jurnal Farmasi Indonesia 19: 193-199.
Alifia AS, Wurlina W, Soeharsono S, Hernawati T, Sudjarwo SA, Utomo B, Mulyati S, Purnama MTE. 2023. Effect of white guava (Psidium guajavaL.) fruit juice on the quality of lead acetate induced rats (Rattus norvegicus) spermatozoa. Ovozoa : J Anim Reprod. 12:123-132.
Barros Adwell CMQ, Brito LFC, Oba E, Wilde RE, Rizzoto G, Thundathil JC, Kastelic JP. 2018. Arterial blood flow is the main source of testicular heat in bulls and higher ambient temperatures significantly increase testicular blood flow. Theriogenology. 116:12-16.
Ben Moula A, Moussafir Z, Hamidallah N, El Amiri B. 2024. Heat stress and ram semen production and preservation: Exploring impacts and effective strategies. J Therm Biol. 119:103794.
Brownlee KK, Moore AW, Hackney AC. 2005. Relationship between circulating cortisol and testosterone: influence of physical exercise. J Sports Sci Med. 4:76-83.
Capela L, Leites I, Romí£o R, Lopes-da-Costa L, Pereira RMLN. 2022. Impact of Heat Stress on Bovine Sperm Quality and Competence. Animals 12(8):975.
Durairajanayagam D, Agarwal A, Ong C. 2015. Causes, effects and molecular mechanisms of testicular heat stress. Reprod Biomed Online. 30:14-27.
Ernawati, Nurliani, A. 2012. Antioxidant Effect of Eleutherine Americana Merr (Bawang Dayak) Ethanol Extract towards the Tubule Seminiferous Microanatomy Structure Testis of Male Rats Exposed to Cigarette Smoke. Jurnal Ilmiah Berkala Sains Dan Terapan Kimia, 6(2): 93– 100.
Gao Y, Wang C, Wang K, He C, Hu K, Liang M. 2022. The effects and molecular mechanism of heat stress on spermatogenesis and the mitigation measures. Sys Biol Reprod Med. 68: 331-347.
Gloria A, Candeloro L, Wegher L, Robbe D, Carluccio A, Contri A. 2021. Environmental temperature and relative humidity differently affect the sperm characteristics in Brown Swiss and Belgian Blue bulls. Int J Biometeorol. 65:2189-2199.
Hassanpour SH, Doroudi A. 2023. Review of the antioxidant potential of flavonoids as a subgroup of polyphenols and partial substitute for synthetic antioxidants. Avicenna J Phytomed. 13:354-376.
Hussain T, Kandeel M, Metwally E, Murtaza G, Kalhoro DH, Yin Y, Tan B, Chughtai MI, Yaseen A, Afzal A, Kalhoro MS. 2023. Unraveling the harmful effect of oxidative stress on male fertility: A mechanistic insight. Front Endocrinol. 14:1070692.
Iwansyah AC, Julianti WP, Luthfiyanti R. 2019. Characterization of nutrition, antioxidant properties, and toxicity of Physalis angulata L. plant extract. Asian J Pharm Clin Res. 12:95-99.
Jena AB, Samal RR, Bhol NK, Duttaroy AK. 2023. Cellular Red-Ox system in health and disease: The latest update. Biomed Pharmacother 162: 114606.
Juan CA, Pérez de la Lastra JM, Plou FJ, Pérez-Lebeña E. 2021. The Chemistry of Reactive Oxygen Species (ROS) Revisited: Outlining Their Role in Biological Macromolecules (DNA, Lipids and Proteins) and Induced Pathologies. Int J Mol Sci. 22:4642.
Kaiser M, Jaillardon L. 2023. Pathogenesis of the crosstalk between reproductive function and stress in animals-part 1: Hypothalamo-pituitary-adrenal axis, sympatho-adrenomedullary system and kisspeptin. Reprod Domest Anim. 58:176-183.
Kastelic JP, Rizzoto G, Thundathil J. 2018. Review: Testicular vascular cone development and its association with scrotal thermoregulation, semen quality and sperm production in bulls. Animal. 12:s133-s141.
Kusumaningtyas RW, Lailya N, Limandha P. 2015. Potential of Ciplukan (Physalis angulata L.) as Source of Functional Ingredient. Procedia Chemistry 14: 367–372.
Llamas-Luceño N, Hostens M, Mullaart E, Broekhuijse M, Lonergan P, Van Soom A. 2020. High temperature-humidity index compromises sperm quality and fertility of Holstein bulls in temperate climates. J Dairy Sci. 103:9502-9514.
Mardatillah M, Wurlina W, Yudaniayanti IS, Plumeriastuti H, Primarizky H, Hamid IS. 2022. Moringa oleiferaleaf extract restored the diameter and thickness of the seminiferous tubules of rat (Rattus norvegicus) injected with gentamicin.Ovozoa : Journal of Animal Reproduction11: 15-21.
Mohamed RH, Mohamed RS, El-Hamid ISA, Madkour FA, Sallam AM, Ali F, Hussein HA. 2023. Semen quality, testicular characteristic, biochemical profile and histopathology of testes of goats under heat stress conditions. Assiut Vet. Med. J.. 69: 76-87
Monageng E, Offor U, Takalani NB, Mohlala K, Opuwari CS. 2023. A Review on the Impact of Oxidative Stress and Medicinal Plants on Leydig Cells. Antioxidants 12:1559.
Ngoula F, Lontio FA, Tchoffo H, Manfo Tsague FP, Djeunang RM, Vemo BN, Moffo F, Djuissi Motchewo N. 2020. Heat Induces Oxidative Stress: Reproductive Organ Weights and Serum Metabolite Profile, Testes Structure, and Function Impairment in Male Cavy (Cavia porcellus). Front Vet Sci. 26;7:37.
Octaviani NAA, Utomo B, Sunarso A, Hamid IS, Madyawati SP,Suprayogi TW. 2021. The effect of Moringa (Moringa oleiferaLam.) leafextract on the motility and viability of spermatozoaof heat exposed rats(Rattus norvegicus). Ovozoa: J Anim Reprod 10: 65-71.
Panggalih A, Susilowati S, Maslachah L, Ratnani H, Suprayogi TW. 2021. The effect of watermelon (Citrullus lanatus) rind ethanolic extracton the number of Leydig,Sertoli, and spermatogenic cells of rat (Rattusnovergicus) exposed to heat. Ovozoa J Anim Reprod. 10: 7-11.
Pham S, Schultz JS. 2021. Testicular thermoregulation with respect to spermatogenesis and contraception. J Therm Biol. 99:102954.
Prastyaningtyas K, Kurnijasanti R, Susilowati S, Suprayogi TW, Eliyani H, Sugihartuti R. 2021. The effect of red dragon fruit (Hylocereus polyrhizus) peel extract on Leydigcells number, seminiferous tubules diameter, and testicular weight ofmice(Mus musculus) exposed to heat. Ovozoa: J Anim Reprod 10: 18-24.
Prevatto JP, Torres RC, Diaz BL, Silva PMRE, Martins MA, Carvalho VF. 2017. Antioxidant Treatment Induces Hyperactivation of the HPA Axis by Upregulating ACTH Receptor in the Adrenal and Downregulating Glucocorticoid Receptors in the Pituitary. Oxid Med Cell Longev. 2017:4156361.
Pugliese MN, Gonzalez D, Jamardo JJ, Ariagno JI, Repetto H, Jacobsen D, Repetto EM, Berg G, Fabre B, Mendeluk GR. 2018. Association of Cortisol and Vitamin D in Seminal Plasma with Sperm Quality. Austin Andrology. 3(2): 1025.
Ramakrishna Pillai J, Wali AF, Menezes GA, Rehman MU, Wani TA, Arafah A, Zargar S, Mir TM. 2022. Chemical Composition Analysis, Cytotoxic, Antimicrobial and Antioxidant Activities of Physalis angulata L.: A Comparative Study of Leaves and Fruit. Molecules. 27:1480.
Roychoudhury S, Chakraborty S, Choudhury AP, Das A, Jha NK, Slama P, Nath M, Massanyi P, Ruokolainen J, Kesari KK. 2021. Environmental Factors-Induced Oxidative Stress: Hormonal and Molecular Pathway Disruptions in Hypogonadism and Erectile Dysfunction. Antioxidants10:837.
Sari NM, Yuliani GA, Yimer N, Hernawati T, Herupradoto EBA, Hidayatik N. 2023. Reddragon (Hylocereus polyrhizus) fruit peel extract increased the motility and viability of spermatozoa of hypercholesterolemic rats (Rattus norvegicus). Ovozoa:Journal of Animal Reproduction,12: 33-41.
Speisky H, Shahidi F, Costa de Camargo A, Fuentes J. 2022. Revisiting the Oxidation of Flavonoids: Loss, Conservation or Enhancement of Their Antioxidant Properties. Antioxidants 11:133.
Sun L, Cui Z, Huang S, Xue Q, Rehman SU, Luo X, Shi D, Li X. 2022. Effect of environmental temperature on semen quality and seminal plasma metabolites of Mediterranean buffalo bulls. Anim Biotechnol. 33:970-980.
Utomo B, Daningtia NR, Yuliani GA, Yuniarti WM. 2019. Effects of a standardized 40% ellagic acid pomegranate (Punica granatum L.) extract on seminiferous tubule histopathology, diameter, and epithelium thickness in albino Wistar rats after heat exposure. Vet World. 12:1261-1265.
Copyright (c) 2024 Annisa Nur Fitria Rahma, Sherina Lashita Candrakirana, Rimayanti Rimayanti, Tri Wahyu Suprayogi, Budi Utomo, Tatik Hernawati, Anwar Ma'ruf, Nove Hidajati, Kadek Rachmawati, Eka Pramyrtha Hestianah, Hani Plumeriastuti
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Ovozoa by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under "the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.
