Success rate of artificial insemination using sexed semen in dairy cows at the Setia Kawan dairy farmers cooperative, Pasuruan, Indonesia
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This study aimed to evaluate the success rate of artificial insemination (AI) using sexed semen in dairy cows, based on the parameters of services per conception (S/C), conception rate (CR), and calf sex ratio. A descriptive method was employed, utilizing both primary and secondary data. Primary data were collected through structured interviews with farmers using questionnaires, while secondary data were obtained from institutional records of the Setia Kawan dairy farmers cooperative, located in Tutur District, Pasuruan Regency, East Java, Indonesia. A total sample of 250 dairy cows was selected, representing 15% of the 1,669 cows that had undergone AI with sexed semen. All sampled cows had at least one prior calving and were owned by members of the cooperative, with AI performed between 2021 and 2022. Artificial insemination was conducted by certified inseminators with over five years of experience, within 6-12 hours of estrus detection. The results showed an S/C value of 1.308 (327 inseminations for 250 cows) and a CR of 81.60% (204 pregnancies from 250 inseminations). Of the 250 calves born, 85.2% (213) were female and 14.8% (37) were male, resulting in a female-to-male sex ratio of 5.76:1. These findings suggest that AI using sexed semen can significantly improve both reproductive efficiency and the production of female offspring in dairy herds.
Ali S, Dirre M, Funga A, Degefa T, Mohammed A, Yeshaneh A. 2024. Behavioral and physical signs of estrous manifested in hormonally estrus-induced Boran and Holstein Friesian dairy cattle. EAS J Vet Med Sci. 6: 1-8.
Álvarez Gallardo H, Urbán Duarte D, Velázquez Roque A, De La Torre Sánchez JF. 2024. Use and evolution of sperm sexing in cattle. Review. Rev Mex Cienc Pecu. 15: 641-68.
Ayantoye JO, Kolachi HA, Zhang X, Shahzad M, Kandil OMT, Wan P, Zhao X. 2025. Integrating omics technologies for enhanced reproductive efficiency in dairy cattle. Animals (Basel) 15: 816.
Bayril T. 2023. Effects of use of conventional and sexed semen on conception rate, calf sex, calf birth weight, and stillbirth in Holstein heifers. Turk J Vet Anim Sci. 47: 108-17.
Britt JH, Cushman RA, Dechow CD, Dobson H, Humblot P, Hutjens MF, Jones GA, Ruegg PS, Sheldon IM, Stevenson JS. 2018. Invited review: Learning from the future-A vision for dairy farms and cows in 2067. J Dairy Sci. 101: 3722-41.
De Vries A, Overton M, Fetrow J, Leslie K, Eicker S, Rogers G. 2008. Exploring the impact of sexed semen on the structure of the dairy industry. J Dairy Sci. 91: 847-56.
Fukaya S, Yamazaki T, Abe H, Nakagawa S, Baba T, Bai H, Takahashi M, Kawahara M. 2024. Characterization of conception rate after embryo transfer in comparison with that after artificial insemination in dairy cattle. J Dairy Sci. 107: 9516-26.
Gil M, Rudy M, Duma-Kocan P, Stanisławczyk R, Krajewska A, Dziki D, Hassoon WH. 2024. Sustainability of alternatives to animal protein sources, a comprehensive review. Sustainability 16: 7701.
Gunawan M, Kaiin EM, Said S. 2015. Application of artificial insemination with sperm sexing to increase cattle productivity in traditional livestock. Pros Sem Nas Masy Biodiv Indon 1: 93-6. DOI
Haile-Mariam M, Pryce JE. 2021. Use of insemination data for joint evaluation of male and female fertility in predominantly seasonal-calving dairy herds. J Dairy Sci. 104: 11807-19.
Hamid M, Abduraman S, Tadesse B. 2021. Risk factors for the efficiency of artificial insemination in dairy cows and around Haramaya town, Oromia region, Eastern Ethiopia. Vet Med Int. 2021: 6622487.
Holden SA, Butler ST. 2018. Review: Applications and benefits of sexed semen in dairy and beef herds. Animal 12: s97-s103.
Howlader MMR, Rahman MM, Hossain MG, Hai MA. 2019. Factors affecting conception rate of dairy cows following artificial insemination in selected selected area at Sirajgonj district of Bangladesh. Biomed J Sci Tech Res. 13: 9907-14.
Jemal H, Lemma A. 2015. Review on major factors affecting the successful conception rates on biotechnological application (AI) in cattle. Glob J Vet Sci Vet Med. 15: 28-37.
Joezy-Shekalgorabi S, Maghsoudi A, Mansourian MR. 2017. Reproductive performance of sexed versus conventional semen in Holstein heifers in various semiarid regions of Iran. Ital J Anim Sci. 16: 666-72.
Kim IH, Jeong JK. 2019. Risk factors limiting first service conception rate in dairy cows and their economic impact. Asian-Australas J Anim Sci. 32: 519-26.
Kuswati K, Prasetyo D, Yekti APA, Susilawati T. 2022. The relationship of estrous character with conception rate in Madura cattle. J Ilmu-Ilmu Peternakan 32: 407-15.
López-Gatius F. 2022. Revisiting the Timing of insemination at spontaneous estrus in dairy cattle. Animals 12: 3565.
Maher JW, Clarke A, Byrne AW, Doyle R, Blake M, Barrett D. 2021. Exploring the opinions of Irish dairy farmers regarding male dairy calves. Front Vet Sci. 8: 635565.
Meles DK, Mustofa I, Hariadi M, Wurlina W, Susilowati S, Amaliya A, Suparto S, Rimayanti R. 2022. The enriched Y-bearing sperm combined with delayed fixed-time artificial insemination for obtaining male Simmental crossbred offspring. Vet World 15: 102-9..
Mendes AJ, Murphy MR, Casper DP, Erickson PS. 2022. The female to male calf sex ratio is associated with the number of services to achieve a calf and parity of lactating dairy cows. Transl Anim Sci. 6: txac080.
Mikkola M, Desmet KLJ, Kommisrud E, Riegler MA. 2024. Recent advancements to increase success in assisted reproductive technologies in cattle. Anim Reprod. 21: e20240031.
Novita CI, Zika M, Sari EM. 2022. Evaluation of artificial insemination program on local cattle in Pante Bidari district, East Aceh regency, Aceh province. J Kedokteran Hewan 16: 73-80.
Quelhas J, Pinto-Pinho P, Lopes G, Rocha A, Pinto-Leite R, Fardilha M, Colaço B. 2023. Sustainable animal production: exploring the benefits of sperm sexing technologies in addressing critical industry challenges. Front Vet Sci. 10: 1181659.
Reese S, Pirez MC, Steele H, Kölle S. 2021. The reproductive success of bovine sperm after sex-sorting: a meta-analysis. Sci Rep. 11: 17366.
Safa F, Firdaus A, Utami P, Yekti APA, Rachmawati A, Susilawati T. 2025. Quality and proportion of X and Y spermatozoa using different sexed methods in Friesian Holstein cattle. Advances in Biological Sciences Research 45: 76-85.
Souames S, Berrama Z. 2020. Factors affecting conception rate after the first artificial insemination in a private dairy cattle farm in North Algeria. Vet World 13: 2608-11.
Stevenson MA. 2021. Sample size estimation in veterinary epidemiologic research. Front Vet Sci. 7: 539573.
Susilawati T, Bustari WO, Crisara IPB, Kuswati, Huda AN, Satria AT, Yekti APA. 2023. Sex ratio of calves resulted from artificial insemination implementation using sexed semen with Percoll gradient density centrifugation method in Ongole crossbred cows. Indian J Anim Res. 57: 841-4.
Ule A, Erjavec K, Klopčič M. 2024. Farmers' preferences for breeding goal traits and selection indexes for Slovenian dairy cattle. J Dairy Sci. 107: 412-22.
Ulfah NA, Samik A, Hariadi M, Suprayogi TW, Hidanah S. 2022. Correlation of parity and age to services per conception, conception rate, and gestation length in Holstein Friesian cross cows. Ovozoa: J Anim Reprod. 11: 9-14.
Wilson DJ, Pempek JA, Cheng TY, Habing G, Proudfoot KL, Winder CB, Renaud DL. 2023. A survey of male and female dairy calf care practices and opportunities for change. J Dairy Sci. 106: 703-17.
Yekti APA, Hasan I, Hermanto H, Febrianto N, Isnaini N, Utami P, Syah HA, Wahjuningsih S, Susilawati T. 2024. The successful artificial insemination with frozen semen from different genetic qualities of Friesian Holstein bull. Indones J Anim Sci. 34: 448-54.
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