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Identifikasi Bakteri Endofit Mangrove Api-Api Putih (Avicennia marina) Penghasil Enzim L-asparaginase
[Identification of Mangrove Endophyte Bacteria of Api-Api Putih (Avicennia marina) as Producing L-asparaginase Enzyme]
Corresponding Author(s) : Asep Awaludin Prihanto
Jurnal Ilmiah Perikanan dan Kelautan,
Vol. 10 No. 2 (2018): Jurnal ilmiah perikanan dan kelautan
Abstract
Abstract
The L-asparaginase enzyme (E.C 3.5.1.1) is a tetramer protein belonging to the family group of amidohydrolases. L-asparaginase selectively hydrolyzes L-aspargin (L-Asn) to ammonia (NH3) and aspartic acid. This study aims to obtain species of L-asparaginase enzyme producing bacteria isolated from Avicennia marina mangroves from Bajul Mati Beach, Malang, East Java, Indonesia. This research carried out several stages such as took and prepared samples, screened L-asparaginase, and identified using the mycrobact system test. The results showed that the isolates with the largest L-asparaginase enzyme activity were DAM6 and it was originated from the leaves of Avicennia marina. DAM6 isolate is a gram-negative with an oval shape, uneven edges, and convex elevation. Based on microbact analysis, DAM6 isolates are Pseudomonas aeruginosa.
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- Abdel, F. Y. R., & Olama, Z. A. (2002). L- asparaginase production by Pseudomonas aeroginosa by solid-state culture: evaluation and optimization culture conditions using factorial designs. Process Biochemistry, 38:115-22.
- El-Bossoumy, A. A., Sarhan, M., & Mansor, J. (2004). Production, isolation, and purification of l-asparaginase from Pseudomonas aeruginosa 50071 using solid-state fermentation. Journal of Biochemistry and Molecular Biology, 4 (37):387-393.
- Gulati, R., Saxena, R. K., & Gupta, R. (1997). A Rapid Plate Assay for Screening L-Asparaginase Producing Micro-Organisms. Letter in Application Microbiology, 24(1):23-26.
- Hendriksen, H. V., Kornbrust, B. A., Ostergaard, P. R., & Mary, A. (2009). Evaluating the potential for enzymatic acrylamide mitigation in a range of food products using an asparaginase from Aspergillus oryzae. Journal of Agricultural and Food Chemistry, 57:4168-4176.
- Jawez, E., Melnick, & Adelberg. (2001). Medical Microbiology 22 Edition. USA: McGraw- Hill Companies.
- Kurniawan, A., Prihanto, A. A., Sari, S. P., Febriyanti, D., Sambah, A. B., & Asriani, E. (2018, April). Isolation and identification of cellulolytic bacteria from mangrove sediment in bangka island. Paper presented at the International Fisheries Symposium, University of Brawijaya, Indonesia.
- Medrofa, A. N., Isidora, K. S., & Mulawarmanti, D. (2015). Ekstrak daun mangrove (A. marina) mempercepat kesembuhan ulkus traumatikus. Jurnal Dentofasial, 14(1):11-14.
- Nur, M., Nasruddin, & Wasiq, J. (2013). Penerapan teknologi plasma untuk mempercepat persemaian mangrove sebagai upaya rehabilitasi green belt untuk mengatasi abrasi. Jurnal Riptek, 7(1):15-26.
- Nurdiani, R., Firdaus, M., & Prihanto, A. A. (2012). Phytochemical screening and Antibacterial activity of methanol extract of mangrove plant (Rhyzophora mucronata) from Porong River Estuary. Journal Basic Science And Technology, 1(2):27-29
References
Abdel, F. Y. R., & Olama, Z. A. (2002). L- asparaginase production by Pseudomonas aeroginosa by solid-state culture: evaluation and optimization culture conditions using factorial designs. Process Biochemistry, 38:115-22.
El-Bossoumy, A. A., Sarhan, M., & Mansor, J. (2004). Production, isolation, and purification of l-asparaginase from Pseudomonas aeruginosa 50071 using solid-state fermentation. Journal of Biochemistry and Molecular Biology, 4 (37):387-393.
Gulati, R., Saxena, R. K., & Gupta, R. (1997). A Rapid Plate Assay for Screening L-Asparaginase Producing Micro-Organisms. Letter in Application Microbiology, 24(1):23-26.
Hendriksen, H. V., Kornbrust, B. A., Ostergaard, P. R., & Mary, A. (2009). Evaluating the potential for enzymatic acrylamide mitigation in a range of food products using an asparaginase from Aspergillus oryzae. Journal of Agricultural and Food Chemistry, 57:4168-4176.
Jawez, E., Melnick, & Adelberg. (2001). Medical Microbiology 22 Edition. USA: McGraw- Hill Companies.
Kurniawan, A., Prihanto, A. A., Sari, S. P., Febriyanti, D., Sambah, A. B., & Asriani, E. (2018, April). Isolation and identification of cellulolytic bacteria from mangrove sediment in bangka island. Paper presented at the International Fisheries Symposium, University of Brawijaya, Indonesia.
Medrofa, A. N., Isidora, K. S., & Mulawarmanti, D. (2015). Ekstrak daun mangrove (A. marina) mempercepat kesembuhan ulkus traumatikus. Jurnal Dentofasial, 14(1):11-14.
Nur, M., Nasruddin, & Wasiq, J. (2013). Penerapan teknologi plasma untuk mempercepat persemaian mangrove sebagai upaya rehabilitasi green belt untuk mengatasi abrasi. Jurnal Riptek, 7(1):15-26.
Nurdiani, R., Firdaus, M., & Prihanto, A. A. (2012). Phytochemical screening and Antibacterial activity of methanol extract of mangrove plant (Rhyzophora mucronata) from Porong River Estuary. Journal Basic Science And Technology, 1(2):27-29