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Uji Aktivitas Antifungi Ekstrak Rimpang Kencur (Kaempferia galanga L.) Terhadap Saprolegnia sp. Secara In Vitro
[Antifungal Activity Test Of Kaempferia galanga L. Extract To Saprolegnia sp. By In Vitro]
Corresponding Author(s) : Rahayu Kusdarwati, Ayu Ratnaningtyas, Dewa Ketut Meles
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 5 No. 1 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
Saprolegnia sp. is a fungus that causes the Saprolegniasis disease can infection eggs and fresh water fish. Treatment Saprolegniasis done using chemical drugs, however the use of drugs is bad for the environment and biota. The purpose of the research was to determined the antifungal activity include a minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) from Kaempferia galanga L. to Saprolegnia sp. by in vitro. This research used 9 different concentrations of Kaempferia galanga L extract were 50%, 12.5%, 6.25%, 3.12%, 1.56%, 0.78%, 0, 39%, 0.2%, positive control used H2O2 3% and negative control used DMSO 10%. The results showed that the extract of Kaempferia galanga L had an antifungal activity were inhibits and kill with minimum inhibitory concentration (MIC) was 0.39% equivalen with 3,9 mg/ml and minimu fungicidal concentration (MBC) was 1.56% equivalen with 15,6 mg/ml. The existence of antifungal activity against Saprolegnia sp. by in vitro caused by some active compounds from the extracts of the Kaempferia galanga L. are polyphenolic compounds, flavonoin, saponins and essential oils.
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- Agoes, A. 2010. Tanaman Obat Indonesia. Salemba Medika. Jakarta. Hal. 57-58
- Akbar, J. 2008. Pemanfaatan Ekstrak Bunga Kecombrang (Nicolaia speciosa Horan) Terhadap Penyembuhan Infeksi Jamur Saprolegnia sp. Pada Ikan Nila Merah. Fakultas Perikanan Universitas Lambung Mangkurat. 26 : 71.
- Assidqi, K. 2012. Potensi Ekstrak Daun Patikan Kebo (Euphorbia hirta) Sebagai Antibakteri Terhadap Aeromonas hydrophila Secara In Vitro. Skripsi. Universitas Airlangga. Surabaya. Hal. 12
- Barus, R. 2009. Amidasi Etil P-Metoksisinamat yang diisolasi Dari Kencur (Kaempferia galangal Linn). Tesis. Universitas Sumatera Utara. Medan. Hal. 23
- Bruno D. W and Woo, P. T. K.. 1999. Fish Disease and Disorders vol. 3 (Viral, Bacterial and Fungal Infection). CAB International Publishing. Wallingford, UK. p : 23-36, 366-432.
- Gandahusada S., D.H. Llahude dan W. Pribadi. 2002. Parasitologi Kedokteran Edisi III. Fakultas Kedokteran Universitas Indonesia. Hal. 10 - 12
- Gholib D, 2009. Daya Hambat Ekstrak Kencur (Kaempferia Galanga L.) Terhadap Trichophyton Mentagrophytes Dan Cryptococcus Neoformans Jamur Penyebab Penyakit Kurap Pada Kulit Dan Penyakit Paru. Balai Besar Penelitian Veteriner. Buletin Littro. Vol. 20 No. 1 : 59 – 67
- Hezmela, R. 2006. Daya Antijamur Ekstrak Lengkuas Merah (Alpinia purpurata K. Schum) Dalam Sediaan Salep. Skripsi. Fakultas Teknologi Pertanian Institut Pertanian Bogor. Bogor. Hal. 40-41
- Kamaludin, I. 2011. Efektivitas Ekstrak Lidah Buaya Aloe Vera Untuk Pengobatan Infeksi Aeromonas Hydrophila Pada Ikan Lele Dumbo Clarias sp. melalui Pakan. Fakultas Perikanan Dan Ilmu Kelautan Institut Pertanian Bogor. Bogor. Hal 25.
- Kusriningrum. 2008. Dasar Perancangan Percobaan dan Rancangan Acak Lengkap. Fakultas Kedokteran Hewan. Universitas Airlangga. Surabaya. Hal 53-92.
- Markham K.R. 1988. Cara Mengindentifikasi Flavonoid. Terjemahan Kosasih Padmawinata. ITB: Bandung
- Mayer, K. 2005. Saprolegnia: There's a fungus among us. http://www.tnfish.org/disease/Saprolegnia.pdf. [ 3 September 2012 ]
- Ogbulie, J. N, C. C. Ogueke, I. C. Okoli and B. N Anyanwu. 2007. Antibacterial Activities and Toxicological Potentials of Crude Ethanolic Extracts of Euphorbia hirta. African Journal of Biotechnology. Vol. 6 (13). pp. 1544-1548.
- Osman, H,M., W.E. Solman, A.E. Noor E.D., and Laila A. 2008. Mohamed. Induction of Saprolegniasis in Oreochromis Niloticus with Special Reference to Its Biology Control. Hydrobiology department, National Research Center, Dokky, Gizza. Global Veterenaria. Egypt. 2(1): 32-37.
- Panda, K., S.S. Brahma and K. Dutta, S. 2010. Selective Antifungal Action of Crude Extracts of Cassia fistula L.A Preltminary Study on Candida and Aspergillus spesies. Journal of Microbiology. Malaysia. 6(1):62- 68.
- Reapina M.E. 2007. Kajian Aktivitas Antimikroba Ekstrak Kulit Kayu Mesoyi (Cryptocaria Massoia) Terhadap Bakteri Patogen Dan Pembusuk Pangan. Skripsi. Fakultas Teknologi Pertanian Institut Pertanian Bogor. Bogor : 30 – 32
- Ridawati, Betty S. L.,J., Ita D. dan Wellyzar S. 2011. Aktivitas Antifungal Minyak Atsiri Jinten Putih Terhadap Candida Parapsilosis SS25, C. Orthopsilosis NN14, C. Metapsilosis MP27, Dan C. Etchellsii MP18.
- Rostiana, O., Rosita SMD., Mono R. dan Taryono. 2005. Budidaya Tanaman Kencur. Balai Penelitian Tanaman Obat dan Aromatika. Sirkuler: 11.
- Setiyani, A. 2010. Uji Aktivitas Antijamur Α-Mangostin Hasil Isolasi Kulit Buah Manggis (Garcinia Mangostana L) Terhadap Malassezia Sp. Skripsi Fakultas Farmasi. Universitas Muhammadiyah Surakarta. Surakarta. Hal. 12
- Sirikan, P. 1981. Parasites Found in Some Indonesian Foshes. Thai Fish Gazette 34. Hal. 345 – 352
- Stoskopf M.K. 1993. Fish Medicine. WB Saunders Company. Philadelphia. Hal 8
- Subandi. 2010. Mikrobiologi. PT Remaja Rosdakarya. Bandung. Hal 91.
- Sulistyawati, D. dan Mulyati, S. 2009. Uji Aktivitas Antijamur Infusa Daun Jambu Mete (Ana ca rdium o ccidentale, L.) terhadap Candida albicans. Biomedika. 2(1): 47-51.
- Yuasa K., Novita P.,Meliya P., dan Edy B.K. 2003. Teknik Diagnosis Penyakit Ikan Budidaya Air Tawar di Indonesia. Japan International Cooperation Agency dan Panduan Diagnosis Penyakit Ikan. Balai Budidaya Air Tawar Jambi. hal. 52-53.
References
Agoes, A. 2010. Tanaman Obat Indonesia. Salemba Medika. Jakarta. Hal. 57-58
Akbar, J. 2008. Pemanfaatan Ekstrak Bunga Kecombrang (Nicolaia speciosa Horan) Terhadap Penyembuhan Infeksi Jamur Saprolegnia sp. Pada Ikan Nila Merah. Fakultas Perikanan Universitas Lambung Mangkurat. 26 : 71.
Assidqi, K. 2012. Potensi Ekstrak Daun Patikan Kebo (Euphorbia hirta) Sebagai Antibakteri Terhadap Aeromonas hydrophila Secara In Vitro. Skripsi. Universitas Airlangga. Surabaya. Hal. 12
Barus, R. 2009. Amidasi Etil P-Metoksisinamat yang diisolasi Dari Kencur (Kaempferia galangal Linn). Tesis. Universitas Sumatera Utara. Medan. Hal. 23
Bruno D. W and Woo, P. T. K.. 1999. Fish Disease and Disorders vol. 3 (Viral, Bacterial and Fungal Infection). CAB International Publishing. Wallingford, UK. p : 23-36, 366-432.
Gandahusada S., D.H. Llahude dan W. Pribadi. 2002. Parasitologi Kedokteran Edisi III. Fakultas Kedokteran Universitas Indonesia. Hal. 10 - 12
Gholib D, 2009. Daya Hambat Ekstrak Kencur (Kaempferia Galanga L.) Terhadap Trichophyton Mentagrophytes Dan Cryptococcus Neoformans Jamur Penyebab Penyakit Kurap Pada Kulit Dan Penyakit Paru. Balai Besar Penelitian Veteriner. Buletin Littro. Vol. 20 No. 1 : 59 – 67
Hezmela, R. 2006. Daya Antijamur Ekstrak Lengkuas Merah (Alpinia purpurata K. Schum) Dalam Sediaan Salep. Skripsi. Fakultas Teknologi Pertanian Institut Pertanian Bogor. Bogor. Hal. 40-41
Kamaludin, I. 2011. Efektivitas Ekstrak Lidah Buaya Aloe Vera Untuk Pengobatan Infeksi Aeromonas Hydrophila Pada Ikan Lele Dumbo Clarias sp. melalui Pakan. Fakultas Perikanan Dan Ilmu Kelautan Institut Pertanian Bogor. Bogor. Hal 25.
Kusriningrum. 2008. Dasar Perancangan Percobaan dan Rancangan Acak Lengkap. Fakultas Kedokteran Hewan. Universitas Airlangga. Surabaya. Hal 53-92.
Markham K.R. 1988. Cara Mengindentifikasi Flavonoid. Terjemahan Kosasih Padmawinata. ITB: Bandung
Mayer, K. 2005. Saprolegnia: There's a fungus among us. http://www.tnfish.org/disease/Saprolegnia.pdf. [ 3 September 2012 ]
Ogbulie, J. N, C. C. Ogueke, I. C. Okoli and B. N Anyanwu. 2007. Antibacterial Activities and Toxicological Potentials of Crude Ethanolic Extracts of Euphorbia hirta. African Journal of Biotechnology. Vol. 6 (13). pp. 1544-1548.
Osman, H,M., W.E. Solman, A.E. Noor E.D., and Laila A. 2008. Mohamed. Induction of Saprolegniasis in Oreochromis Niloticus with Special Reference to Its Biology Control. Hydrobiology department, National Research Center, Dokky, Gizza. Global Veterenaria. Egypt. 2(1): 32-37.
Panda, K., S.S. Brahma and K. Dutta, S. 2010. Selective Antifungal Action of Crude Extracts of Cassia fistula L.A Preltminary Study on Candida and Aspergillus spesies. Journal of Microbiology. Malaysia. 6(1):62- 68.
Reapina M.E. 2007. Kajian Aktivitas Antimikroba Ekstrak Kulit Kayu Mesoyi (Cryptocaria Massoia) Terhadap Bakteri Patogen Dan Pembusuk Pangan. Skripsi. Fakultas Teknologi Pertanian Institut Pertanian Bogor. Bogor : 30 – 32
Ridawati, Betty S. L.,J., Ita D. dan Wellyzar S. 2011. Aktivitas Antifungal Minyak Atsiri Jinten Putih Terhadap Candida Parapsilosis SS25, C. Orthopsilosis NN14, C. Metapsilosis MP27, Dan C. Etchellsii MP18.
Rostiana, O., Rosita SMD., Mono R. dan Taryono. 2005. Budidaya Tanaman Kencur. Balai Penelitian Tanaman Obat dan Aromatika. Sirkuler: 11.
Setiyani, A. 2010. Uji Aktivitas Antijamur Α-Mangostin Hasil Isolasi Kulit Buah Manggis (Garcinia Mangostana L) Terhadap Malassezia Sp. Skripsi Fakultas Farmasi. Universitas Muhammadiyah Surakarta. Surakarta. Hal. 12
Sirikan, P. 1981. Parasites Found in Some Indonesian Foshes. Thai Fish Gazette 34. Hal. 345 – 352
Stoskopf M.K. 1993. Fish Medicine. WB Saunders Company. Philadelphia. Hal 8
Subandi. 2010. Mikrobiologi. PT Remaja Rosdakarya. Bandung. Hal 91.
Sulistyawati, D. dan Mulyati, S. 2009. Uji Aktivitas Antijamur Infusa Daun Jambu Mete (Ana ca rdium o ccidentale, L.) terhadap Candida albicans. Biomedika. 2(1): 47-51.
Yuasa K., Novita P.,Meliya P., dan Edy B.K. 2003. Teknik Diagnosis Penyakit Ikan Budidaya Air Tawar di Indonesia. Japan International Cooperation Agency dan Panduan Diagnosis Penyakit Ikan. Balai Budidaya Air Tawar Jambi. hal. 52-53.