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Kandungan Gizi dan Karakterisasi Senyawa Bioaktif Lintah Laut (Discodoris sp.)
[Nutritional Content and Characterization Bioactive Compounds of Sea Sluge (Discodoris sp.) ]
Corresponding Author(s) : Hafiluddin Hafiluddin
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
One of the commodities produced from the ocean that have a bioactive compounds is a sea slug (Discodoris sp.). That is very interesting to study mainly deals with the nature of both chemical and biochemical characteristics and their use for the field of food and health. The aims of this research was to determine the nutrient content of sea slug, determaine chemical content and determine antioxidant activity. The experiment was conducted with several stages: sample preparation, analysis of nutrient content, extraction bioactive compound, analysis of chemical content and antioxidant activity. Sea slug from the island of Madura Pamekasan potential as a source of protein, fat, and minerals. Sea slug has esensial amino acids amount to 5.57% was dominated by leucin of 1.42%. Nonesensial amino acids amount to 6.54% wich was dominated by glutamic acid 2.19%. Saturated fatty acids amounted to 27.53% of sea slug was dominated by palmitic (C16: 0) which was 13.36%. Unsaturated fatty acids amounted to 34.66%, which was dominated by the essential fatty acids linolenic (C18: 3, n-3) 20.91%. The highest yield of the crude extract was ethanol and contained alkaloid compounds, steroids, phenols, carbohydrates and reducing sugar compound. The meat of sea slug with ethanol solvent has an IC best 50 antioxidant activity at 441, 12 ppm.
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References
American Association of Cereal Chemist [AACC]. 1983. Approved Methods of The American Association of Cereal Chemist. Ed ke-8. Maret. USA :American Association of Cereal Chemist.
American Association of Cereal Chemist [AACC]. 1994. Approved Methods of The American Association of Cereal Chemist. Ed ke-9. Vol 1. USA :American Association of Cereal Chemist.
Association of Official Analytical Chemist [AOAC]. 2005. Official Methods of Analysis (18 Edn). Association of Official Analytical Chemist Inc. Mayland. USA.
Dewan Standarisasi Nasional [SNI]. 1998. Cara Uji Cemaran Logam dalam Makanan. SNI 01- 2896 - 1998 . Jakarta : Departemen Perindustrian RI.
Dewan Standarisasi Nasional [SNI]. 2009. Batas Maksimum Cemaran Logam Berat dalam Bahan Pangan. SNI 7387-2009. Jakarta: Badan Standarisasi Nasional.
Departemen Kesehatan Republik Indonesia. 1995. Materia Medika Indonesia. Jakarta: Departemen Kesehatan Republik Indonesia.
Hanani E, Mun'im A, Sekarini R. 2005. Identifikasi Senyawa Antioksidan dalam Spons Callyspongia sp. dari Kepulauan Seribu. Majalah Ilmu Kefarmasian, 2(3):127 –133.
Houghton PJ, Raman A. 1998. Laboratory Handbook for the Fractionation of Natutal Extracts. London: Chapman and Hall.
Ibrahim M. 2001. Isolasi dan uji aktivitas biologi senyawa steroid dari lintah laut, Discodoris sp. [Tesis]. Program Pascasarjana. Institut Pertanian Bogor. Bogor.
Molyneux P. 2004. The use of the stable free radicals diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. Technol. 26(2):211-219.
Naiu AS. 2011. Formulasi minuman fungsional berbahan baku lintah laut. [Tesis]. Sekolah Pascasarjana IPB. Bogor.
Nurjanah, Hardjito L, Monintja DR, Bintang M, Agungpriyono DR. 2010. Karakterisasi Lintah Laut (Discodoris sp.) sebagai antioksidan dan antikolesterol.[Disertasi]. Sekolah Pascasarjana IPB. Bogor.
Rohman A, Riyanto R, Diah U. 2006. Aktivitas antioksidan, kandungan fenolik total dan kandungan flavonoid total ekstrak etil asetat buah Mengkudu serta fraksi-fraksinya. Majalah Farmasi Indonesia . 17(3):136–142.
Seidel V. 2006. Initial and bulk ekxtraction. Methods in Biotechnology. Natural Products Isolation. Second edition. 20:27-46.
Sherif S Ebada, Ru Angelie Edrada, Wenhan Lin dan Peter Procksch. 2008. Methods for isolation, purification and structural elucidation of bioactive secondary metabolites from marine invertebrata. Nature Protocols.3(12):1820-1831.