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Pengaruh Pemberian Bakteri Bacillus pumilus pada kotoran Sapi Sebagai Pupuk terhadap Jumlah Kandungan Klorofil Dunaliella salina
[The Effect of Bacteria Bacillus pumilus In Cow Dung As Fertilizer to Total Chlorophyll Dunaliella salina]
Corresponding Author(s) : Endang Dewi Masithah
Jurnal Ilmiah Perikanan dan Kelautan,
Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
Dunaliella salina is one type of natural food used in the hatchery business. Production stability D. salina with an abundance of nutrients can be supported. The purpose of this study was to determine the effect and how many doses of the bacteria Bacillus pumilus best fermented in cow dung as fertilizer in increasing the population of D. salina. Research conducted at the Laboratory of Education Faculty of Fisheries and Marine Fisheries, Airlangga University Surabaya. The research design used was Completely Randomized Design (CRD) is followed by Duncan test. D. salina was cultured in 250 mL glass bottle with 5 treatments (2 treatments as a control) and four replications. Culture medium used contained 10 ppm cow dung. The concentration of cow manure are given in the study, namely A (12.5% Bacillus pumilus), B (10% Bacillus pumilus), C (7.5% Bacillus pumilus), control 1 (without fermentation), control 2 (fertilizer Walne). The results showed that the addition of Bacillus pumilus on cow dung that were cultured in culture medium D. salina can increase the amount of chlorophyll content of D. salina. Addition of Bacillus pumilus by 10% in the culture medium to produce the amount of chlorophyll-a D. salina high of 0.014055 µg/mL and chlorophyll-b of 0.009657142 µg/mL on the first day.
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- Anggadireja, J. T., A. Zatnika., H. Purwanto dan S. Istini. 2006. Rumput Laut. Penebar Swadaya. Jakarta. hal. 39-47.
- Arinardi, O.H., A.B. Sutomo, S.A. Yusuf, Trimaningsih, E. Asnaryanti dan S.H. Riyono, 1997. Kisaran Kelimpahan dan Komposisi Plankton Predominan di Perairan Kawasan Timur Indonesia. Puslitbang Oseanologi-LIPI, Jakarta.
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- Gunawan dan Sundari. 2003. Pengaruh Penggunaan Probiotik dalam Ransum terhadap P r o d u k t i v i t a s A y a m . http://peternakan.litbang.deptan.go.id/133- 2.pdf. 25/10/2009. 7 hal.
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- Kusriningrum, R. 1989. Dasar Perencanaan Percobaan dan Rancangan Acak Lengkap. Universitas Airlangga. Surabaya. hal.53–90.
- Lamid, M., S. Chuzaemi, N. Nyoman T. P. dan Kusmartono. 2006. Inokulasi Bakteri Xilanolitik Asal Rumen sebagai Upaya Peningkatan Nilai Nutrisi Jerami Padi. Fakultas Kedokteran Hewan Universitas A i r l a n g g a . S u r a b a y a . http//www.mirnilamid@yahoo.com. 11/03/2010. 7 hal.
- Nurjariah. 2005. Kelimpahan Bakteri dalam Budidaya Cacing Sutera Limnodrilus sp. Yang Dipupuk Kotoran Ayam Hasil Fermentasi. Skripsi. Institut Pertanian Bogor. Bogor. 34 hal.
- Prasojo, R. 2010. Pengaruh Penggunaan Pupuk Kotoran Ayam yang Difermentasi EM4 Terhadap Pertumbuhan Populasi Dunaliella salina. Skripsi. Fakultas Perikanan dan Kelautan Universitas Airlangga.
- Satyantini, W.H dan E. D. Masithah. 2007. Diktat Penuntun Praktikum Budidaya Pakan Alami. Program Studi Budidaya Perairan Fakultas Kedokteran Hewan Universitas Airlangga. Surabaya. hal. 2 – 28.
- Sterman, T. N. 1988. Spectrophotometric and Fluorometric Chlorophyll Analysis. In : Lobban, S. C., D.J. Chapman and B. P. Kremer. Experimental phycology, A Laboratory Manual Cambridge University Press. New York. P. 35-39
- Yurong, C., L. Yumin, W. Tianyun, H. Weihong and X. Lexun. 2007. Heterologous Gene Expression Driven by Carbonic Anhydrase Gene Promoter in Dunaliella salina. http://www.sciencedirect.com. 25/10/2009.6pp.
References
Anggadireja, J. T., A. Zatnika., H. Purwanto dan S. Istini. 2006. Rumput Laut. Penebar Swadaya. Jakarta. hal. 39-47.
Arinardi, O.H., A.B. Sutomo, S.A. Yusuf, Trimaningsih, E. Asnaryanti dan S.H. Riyono, 1997. Kisaran Kelimpahan dan Komposisi Plankton Predominan di Perairan Kawasan Timur Indonesia. Puslitbang Oseanologi-LIPI, Jakarta.
Denault, M., E. Stieve and I. valiela. 2000. Effects of Nitrogen Load and Irradiance on Photosynthetic Pigment Concentration in Cladophora vagabunda and Gracillari tikhivae in Esturies of Waquoit Bay. Biology Billetins. 199 : 223-225.
Ekawati, A. W. 2005. Diktat Kuliah Budidaya Pakan Alami. Fakultas Perikanan Universitas Brawijaya. Malang. hal. 3 – 48.
Gunawan dan Sundari. 2003. Pengaruh Penggunaan Probiotik dalam Ransum terhadap P r o d u k t i v i t a s A y a m . http://peternakan.litbang.deptan.go.id/133- 2.pdf. 25/10/2009. 7 hal.
Hanisak, 1983. The Nitrogen Relationhips of Marine Macroalgae. In : Carpenter, E. J and D. G. Capone. Nitrogen in The Marine Environment. Academic Press Inc. New York. p. 703
Kusriningrum, R. 1989. Dasar Perencanaan Percobaan dan Rancangan Acak Lengkap. Universitas Airlangga. Surabaya. hal.53–90.
Lamid, M., S. Chuzaemi, N. Nyoman T. P. dan Kusmartono. 2006. Inokulasi Bakteri Xilanolitik Asal Rumen sebagai Upaya Peningkatan Nilai Nutrisi Jerami Padi. Fakultas Kedokteran Hewan Universitas A i r l a n g g a . S u r a b a y a . http//www.mirnilamid@yahoo.com. 11/03/2010. 7 hal.
Nurjariah. 2005. Kelimpahan Bakteri dalam Budidaya Cacing Sutera Limnodrilus sp. Yang Dipupuk Kotoran Ayam Hasil Fermentasi. Skripsi. Institut Pertanian Bogor. Bogor. 34 hal.
Prasojo, R. 2010. Pengaruh Penggunaan Pupuk Kotoran Ayam yang Difermentasi EM4 Terhadap Pertumbuhan Populasi Dunaliella salina. Skripsi. Fakultas Perikanan dan Kelautan Universitas Airlangga.
Satyantini, W.H dan E. D. Masithah. 2007. Diktat Penuntun Praktikum Budidaya Pakan Alami. Program Studi Budidaya Perairan Fakultas Kedokteran Hewan Universitas Airlangga. Surabaya. hal. 2 – 28.
Sterman, T. N. 1988. Spectrophotometric and Fluorometric Chlorophyll Analysis. In : Lobban, S. C., D.J. Chapman and B. P. Kremer. Experimental phycology, A Laboratory Manual Cambridge University Press. New York. P. 35-39
Yurong, C., L. Yumin, W. Tianyun, H. Weihong and X. Lexun. 2007. Heterologous Gene Expression Driven by Carbonic Anhydrase Gene Promoter in Dunaliella salina. http://www.sciencedirect.com. 25/10/2009.6pp.