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Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Metallothionein (MT) Expression and SEM-EDX Mapping on Cymodocea serrulata Seagrass

https://doi.org/10.20473/jipk.v14i2.32531
Dwi Rosalina
Marine Engineering Program, Politeknik Kelautan dan Perikanan Bone, South Sulawesi. Indonesia
https://orcid.org/0000-0002-3402-5295
Dini Sofarini
Management of Aquatic Resources, University of Lambung Mangkurat, South Kalimantan. Indonesia
Firman Farid Muhsoni
Marine Science Program, Faculty of Fisheries and Marine, University of Trunojoyo, East Java. Indonesia
Misbakhul Munir
Marine Science Program, Faculty of Science and Technology, State Islamic University of Sunan Ampel, Surabaya, East Java. Indonesia
Ruly Isfatul Khasanah
UIN Sunan Ampel of Surabaya
https://orcid.org/0000-0002-0921-465X

Corresponding Author(s) : Dwi Rosalina

myrafirifky@gmail.com

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Highlight Research


1. Metallothionein is a protein found in seagrass and functions to bind to heavy metals so as not to damage the seagrass body tissue


 


Abstract


Lead is a very dangerous heavy metal for organisms because it is carcinogenic, can cause mutations, takes a long time to decompose and has unchanging toxicity. This study on the seagrass Cymodocea serrulata aimed to determine the metallothionein expression quantitatively using the Elisa (Enzyme-Linked Immunosorbent Assay) method and describe the surface structure of C. serrulata roots, rhizomes, and leaves that were exposed to lead using SEM and EDX Mapping methods. The results showed that metallothionein was abundant in C. serrulata tissues (leaves>roots>rhizomes) in both South Bangka and Ketawai Island. SEM showed changes in the morphology and cell size of C. serrulata exposed to lead. EDX and Mapping showed the substance elements found in seagrass tissue. Pb was detected in C. serrulata roots (0.22%) and leaves (0.6%) in South Bangka. This indicates that the seagrass plants are able to absorb heavy metals into their body tissues.

Keywords

Cymodocea serrulata elisa metallothionein SEM-EDX mapping
Dwi Rosalina, Sofarini , D. ., Muhsoni, F. F. ., Munir, M. ., & Khasanah, R. I. (2022). Metallothionein (MT) Expression and SEM-EDX Mapping on <I>Cymodocea serrulata</I> Seagrass. Jurnal Ilmiah Perikanan Dan Kelautan, 14(2), 313–326. https://doi.org/10.20473/jipk.v14i2.32531
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Author Biographies

Dwi Rosalina, Marine Engineering Program, Politeknik Kelautan dan Perikanan Bone, South Sulawesi. Indonesia

Marine Engineering Program, Politeknik Kelautan dan Perikanan Bone, South Sulawesi. Indonesia

Dini Sofarini , Management of Aquatic Resources, University of Lambung Mangkurat, South Kalimantan. Indonesia

Management of Aquatic Resources, University of Lambung Mangkurat, South Kalimantan. Indonesia

Firman Farid Muhsoni, Marine Science Program, Faculty of Fisheries and Marine, University of Trunojoyo, East Java. Indonesia

Marine Science Program, Faculty of Fisheries and Marine, University of Trunojoyo, East Java. Indonesia

Misbakhul Munir, Marine Science Program, Faculty of Science and Technology, State Islamic University of Sunan Ampel, Surabaya, East Java. Indonesia

Marine Science Program, Faculty of Science and Technology, State Islamic University of Sunan Ampel, Surabaya, East Java. Indonesia

Ruly Isfatul Khasanah, UIN Sunan Ampel of Surabaya
ilmu kelautan
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