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Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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March 29, 2022
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Effects of Urea Removal on the Stable Isotopes δ13C and δ15N in Rays from the Coastal Waters of Peninsular Malaysia

https://doi.org/10.20473/jipk.v14i2.34682
Maizah Mohd Abdullah
Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia
https://orcid.org/0000-0001-7701-6623
Wei Yien Lua
Institute of Oceanography and Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia
Roslanzairi Mostapha
Environmental Tracer Application Group, Waste and environmental Technology Division, Malaysian Nuclear Agency, Kajang, Selangor, 43000. Malaysia
Zalina Bashir
Institute of Oceanography and Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia
Mohd Naimullah
Department of Environmental Biology and Fisheries Science, National Taiwan Ocean University, Keelung, Taiwan, 20224. Republic of China
Sukree Hajisamae
Prince of Songkla University, Pattani, 94000. Thailand
Fazrul Hisam
Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia

Corresponding Author(s) : Maizah Mohd Abdullah

maizah@umt.edu.my

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Highlight Research



  1. This is the first stable isotopes study of rays from Malaysian waters

  2. Urea extraction significantly enriched the δ15N values ("°)

  3. Urea removal is obliged for stable isotope studies on rays

  4. Such methodological information is critical for marine research in the region


 


Abstract


This is the first reported record of stable isotope values for elasmobranch rays within Malaysian waters, and serves as an important baseline methodological information for future studies investigating stable isotope values in both shark and ray species in the region. This study examined the effects of urea removal on the stable isotopes values of δ13C and δ15N in muscle tissues sampled from several elasmobranch rays species, namely Pastinachus atrus, Rhinoptera javanica, Himantura gerrardi, Himantura uarnak and Aetobatus ocellatus. Individual ray species were collected in July and August of 2018, from the coastal waters of Terengganu State, Malaysia. Urea removal was performed by soaking muscle tissue samples in deionised water for at least 24 hours before being dehydrated. The resulting stable isotope values of these samples were compared to samples that did not undergo the urea removal process. Stable isotope values were individual and species-specific, however, the effect of urea removal was significant for δ15N values but did not significantly affect the δ13C values. We conclude that removing urea from elasmobranch samples before stable isotope analysis is advisable to draw correct conclusions about the animal's diets.

Keywords

elasmobranch trophic ecology Bidong Island Terengganu South China Sea
Abdullah, M. M., Yien Lua, W., Mostapha, R., Bashir, Z., Naimullah, M., Hajisamae, S., & Hisam, F. (2022). Effects of Urea Removal on the Stable Isotopes δ13C and δ15N in Rays from the Coastal Waters of Peninsular Malaysia. Jurnal Ilmiah Perikanan Dan Kelautan, 14(2), 340–348. https://doi.org/10.20473/jipk.v14i2.34682
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Author Biographies

Maizah Mohd Abdullah, Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia

Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia

Institute of Oceanography and Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia

Wei Yien Lua, Institute of Oceanography and Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia

Institute of Oceanography and Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia

Roslanzairi Mostapha, Environmental Tracer Application Group, Waste and environmental Technology Division, Malaysian Nuclear Agency, Kajang, Selangor, 43000. Malaysia

Environmental Tracer Application Group, Waste and environmental Technology Division, Malaysian Nuclear Agency, Kajang, Selangor, 43000. Malaysia

Zalina Bashir, Institute of Oceanography and Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia

Institute of Oceanography and Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia

Mohd Naimullah, Department of Environmental Biology and Fisheries Science, National Taiwan Ocean University, Keelung, Taiwan, 20224. Republic of China

Department of Environmental Biology and Fisheries Science, National Taiwan Ocean University, Keelung, Taiwan, 20224. Republic of China

Sukree Hajisamae, Prince of Songkla University, Pattani, 94000. Thailand

Prince of Songkla University, Pattani, 94000. Thailand

Fazrul Hisam, Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia

Faculty of Fisheries and Food Sciences, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300. Malaysia

Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, 21300.  Malaysia

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