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Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Fisheries Biotechnology Antioxidant, Medicinal Plants, and Antibacterial

The Potency of Water Clover (Marsilea crenata C. Presl.) Leaves as Anticholesterolemic Functional Foods Through In Silico Study

https://doi.org/10.20473/jipk.v15i1.35338
Hardoko Hardoko
Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia
https://orcid.org/0000-0002-0395-9589
Syahrani Nurul Mutmainannah
Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia

Corresponding Author(s) : Hardoko Hardoko

hardoko@ub.ac.id

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Highlight Research


1. There were 26 active compounds from water clover leaf extract that were identified using GC-MS


2. In silico approach was used to screen potential anticholesterolemic compounds from water clover leaf extract


3. Anticholesterolemic compounds from water clover leaf extract were interacted with HMG-CoA reductase receptors and compared to native ligand and medicine


4. There were 6 potential anticholesterolemic compounds identified from water clover leaf extract


 


Abstract


 


Water clover (Marsilea crenata C. Presl.) is a widely available plant in Indonesia and often utilized as a traditional food ingredient. This plant is also traditionally believed to contain compounds that can decrease blood cholesterol. This study aimed to determine the compounds in water clover which have the potential to decrease blood cholesterol through inhibition of the HMG-CoA enzyme using in silico approach. This research was done in several steps, i.e., extraction using ethyl acetate solvent, identification of chemical compounds using GC-MS, and screening of compounds with potential to be  anticholesterolemic agent through in silico using PyRx 0.8 (AutoDockVina and  Open Babel GUI version 2.4.1), Discovery Studio Visualizer 2021, and PyMOLâ„¢ 1.7.4.5 software. Results showed that ethyl acetate extract of water clover contained 26 compounds, 6 of which were potential to be anticholesterolemic agent, i.e., phytol, 1,2-benzenedicarboxylic acid, 2,4-di- tert-butylphenol, diethyl phthalate, 1,2,3,4-tetramethylbenzene, and dipentene. Binding affinity values of those six compounds were lower than the native ligand of the HMG-CoA reductase, although still higher compared to pravastatin. The binding affinity value of pravastatin was -7.13 kcal/mol and the binding affinity value of 3-methyl glutaric acid as a native ligand was -5.33 kcal/mol, meanwhile, the lowest binding affinity value of compounds in water clover was phytol (-6.37 kcal/mol) and the highest was dipentene (-5.40 kcal/mol). Through in silico study, there were six compounds from water clover leaf's ethyl acetate extract that could inhibit the HMG-CoA reductase. Therefore, water clover leaf has the potential to become an anticholesterolemic functional food ingredient.

Keywords

Binding Affinity Extract HMG-CoA Reductase Inhibition
Hardoko, H., & Syahrani Nurul Mutmainannah. (2023). The Potency of Water Clover (<I>Marsilea crenata</I> C. Presl.) Leaves as Anticholesterolemic Functional Foods Through <I>In Silico</I> Study. Jurnal Ilmiah Perikanan Dan Kelautan, 15(1), 131–141. https://doi.org/10.20473/jipk.v15i1.35338
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Author Biographies

Hardoko Hardoko, Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia

Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia

Syahrani Nurul Mutmainannah, Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia

Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia

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