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Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Fisheries Biotechnology Antioxidant, Medicinal Plants, and Antibacterial

The Potency of Water Clover (Marsilea crenata C. Presl.) Leaves as Anticholesterolemic Functional Foods Through In Silico Study

https://doi.org/10.20473/jipk.v15i1.35338
Hardoko Hardoko
Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia
https://orcid.org/0000-0002-0395-9589
Syahrani Nurul Mutmainannah
Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia

Corresponding Author(s) : Hardoko Hardoko

hardoko@ub.ac.id

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Highlight Research


1. There were 26 active compounds from water clover leaf extract that were identified using GC-MS


2. In silico approach was used to screen potential anticholesterolemic compounds from water clover leaf extract


3. Anticholesterolemic compounds from water clover leaf extract were interacted with HMG-CoA reductase receptors and compared to native ligand and medicine


4. There were 6 potential anticholesterolemic compounds identified from water clover leaf extract


 


Abstract


 


Water clover (Marsilea crenata C. Presl.) is a widely available plant in Indonesia and often utilized as a traditional food ingredient. This plant is also traditionally believed to contain compounds that can decrease blood cholesterol. This study aimed to determine the compounds in water clover which have the potential to decrease blood cholesterol through inhibition of the HMG-CoA enzyme using in silico approach. This research was done in several steps, i.e., extraction using ethyl acetate solvent, identification of chemical compounds using GC-MS, and screening of compounds with potential to be  anticholesterolemic agent through in silico using PyRx 0.8 (AutoDockVina and  Open Babel GUI version 2.4.1), Discovery Studio Visualizer 2021, and PyMOLâ„¢ 1.7.4.5 software. Results showed that ethyl acetate extract of water clover contained 26 compounds, 6 of which were potential to be anticholesterolemic agent, i.e., phytol, 1,2-benzenedicarboxylic acid, 2,4-di- tert-butylphenol, diethyl phthalate, 1,2,3,4-tetramethylbenzene, and dipentene. Binding affinity values of those six compounds were lower than the native ligand of the HMG-CoA reductase, although still higher compared to pravastatin. The binding affinity value of pravastatin was -7.13 kcal/mol and the binding affinity value of 3-methyl glutaric acid as a native ligand was -5.33 kcal/mol, meanwhile, the lowest binding affinity value of compounds in water clover was phytol (-6.37 kcal/mol) and the highest was dipentene (-5.40 kcal/mol). Through in silico study, there were six compounds from water clover leaf's ethyl acetate extract that could inhibit the HMG-CoA reductase. Therefore, water clover leaf has the potential to become an anticholesterolemic functional food ingredient.

Keywords

Binding AffinityExtractHMG-CoA ReductaseInhibition
Hardoko, H., & Syahrani Nurul Mutmainannah. (2023). The Potency of Water Clover (<I>Marsilea crenata</I> C. Presl.) Leaves as Anticholesterolemic Functional Foods Through <I>In Silico</I> Study. Jurnal Ilmiah Perikanan Dan Kelautan, 15(1), 131–141. https://doi.org/10.20473/jipk.v15i1.35338
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References
  1. Afriastini, J. J. (2003). Marsilea crenata C Presl. In W. P. de Winter and V. B. Amoroso (Ed.), Cryptogams: Ferns and fern allies. (pp. 133-135). Leiden: Backhuys Publishers.
  2. Al Hageh, C., Rahy, R., Khazen, G., Brial, F., Khnayzer, R. S., Gauguier, D., & Zalloua, P. A. (2020). Plasma and urine metabolomic analysis in aortic valve stenosis reveal shared and biofluid-specific changes in metabolite levels. Plos One, 15(11):1-18.
  3. Alimar, M. F., Nura, M. H., Mayonva, H., Yursal, M. D., Asnur, L., & Kamal, F. K. (2022). (Boring sen) cherry leaf dried sponge is beneficial in maintaining cholesterol levels and high blood pressure. Spectrum, 1(1):36-47.
  4. Alvi, S. S., Iqbal, D., Ahmad, S., & Khan, M. S. (2015). Molecular rationale delineating the role of lycopene as a potent HMG-CoA reductase inhibitor: in vitro and in silico study. Natural Product Research, 30(18):2111-2114.
  5. Anggraini, D. I. & Ali, M. M. (2017). Uji aktivitas antikolesterol ekstrak etanol daun binahong (Anredera cordifolia (Ten) Steenis) secara in vitro. Jurnal Ilmiah Kesehatan, 9(1):1-6.
  6. Anggraini, D. I., & Nabillah, L. F. (2018). Activity test of suji leaf extract (Dracaena angustifolia Roxb.) on in vitro cholesterol lowering. Jurnal Kimia Sains dan Aplikasi, 21(2):54-58.
  7. Arfi, A. S., Lestari, R. D., & Damayanti, D. S. (2020). Studi in silico senyawa aktif rimpang kunyit (Curcuma domestica) terhadap penghambatan acetylcholinesterase, Microtubulin (Beta tubulin), dan aktivasi calcium channel sebagai terapi antelmintik. Jurnal Kedokteran Komunitas, 8(2):36-47.
  8. Arwansyah, A. L., & Hasrianti. (2014). Simulasi molecular docking senyawa kurkumin dan analognya sebagai Selective Androgen Receptor Modulators (SARMs) pada kanker prostat. Jurnal Dinamika, 05(2):60-75.
  9. Bare, Y., Rophi, A. H., Tiring, S. S. N. N. D., Rachmad, Y. T., Nugraha, F. A. D., & Sari, D. R. T. (2019). Prediction potential chlorogenic acid as inhibitor ACE (in silico study). Bioscience, 3(2):197-203.
  10. Borges, R. S., Keita, H., Ortiz, B. L. S., Sampaio, T. I. d. S., Ferreira, I. M., Lima, E. S., da Silva, M. d. J. A., Fernandes, C. P., Oliveira, A. E. M. d. F. M., da Conceiçí£o, E. C., Rodrigues, A. B. L., Filho, A. C. M. P., Castro, A. N., & Carvalho, J. C. T. (2018). Anti-inflammatory activity of nanoemulsions of essential oil from Rosmarinus officinalis L.: in vitro and in zebrafish studies. Inflammopharmacology, 26(2018):1057-1080.
  11. Burg, J. S., & Espenshade, P. J. (2011). Regulation of HMG-CoA reductase in mammals and yeast. Progress in Lipid Research, 50(4):403-410.
  12. Choudhury, S., & Sinha, M. P. (2015). Effect of aqueous extract of Murraya koenigii on haematological, hormonal and lipid profile of albino rats. Journal of Coastal Life Medicine, 3(11):901-905.
  13. Demitri, A., Wirjatmadi, B., & Adriani, M. (2018). Effect of melinjo peel extract on triglycerides level of rats feed high cholesterol diet. International Journal of Public Health and Clinical Sciences, 5(6):301-306.
  14. Djamil, R., Zaidan, S., Butar, V. B., & Pratami, D. K. (2020). Formulasi nanoemulsi ekstrak etanol buah Okra (Abelmoschus esculentus L. Moench.) dan uji aktifitas antikolesterol secara in-vitro. Jurnal Ilmu Kefarmasian Indonesia, 18(1):75-80.
  15. dos Santos, D. C., Lima, A. L., De Sousa, T. L., Santos, N. H., Ochoa, J. Z., Silva, F. G., & Egea, M. B. (2020). Quality parameters and health impact of clarified "Cerrado” cashew juice (Anacardium othonianum Rizz.). Current Developments in Nutrition, 4(2):385.
  16. Eff, A. R. Y., Rahayu, S. T., & Syachfitri, R. D. (2016). Uji aktivitas penghambatan xantin oksidase secara in-vitro oleh isolat 6,4'-Dihidroksi-4-Metoksibenzofenon-2-O-β-D-Glukopiranosida (C20H22O10) yang diisolasi dari mahkota dewa (Phaleria macrocarpa (Scheff.) Boerl). Pharmaceutical Sciences and Research (PSR), 3(1):1-11.
  17. Hafidz, K. A., Puspitasari, N., Azminah, Yanuar, A., Artha, Y., & Mun'im, A. (2017). HMG-CoA reductase inhibitory activity of Gnetum gnemon seed extract and identification of potential inhibitors for lowering cholesterol level. Journal of Young Pharmacists, 9(4):559-565.
  18. Hardoko, Gunawan, W. L., & Handayani, R. (2019). Aktivitas inhibisi ekstrak daun semanggi air (Marsilea crenata) terhadap enzim HMG-KoA reduktase. FaST - Jurnal Sains dan Teknologi, 3(1):45-57.
  19. Hernandez, M. A., & Appu, R. (2006). Basic pharmacology: Understanding drug action and reaction. Boca Raton: CRC Press.
  20. Hartanti, L., Yonas, S. M. K., Mustamu, J. J., Wijaya, S., Setiawan, H. K., & Soegianto, L. (2019). Influence of extraction methods of Bay leaves (Syzygium polyanthum) on antioxidant and HMG-CoA reductase inhibitory activity. Heliyon, 5(4):e01485.
  21. Ilyas, A. N., Rahmawati, & Widiastuti, H. (2020). Uji aktivitas antikolesterol ekstrak etanol daun gedi (Abelmoschus manihot (L.) medik secara in vitro. Jurnal Kesehatan, 3(1):057-064.
  22. Ismail, M., Al-Naqeep, G., & Chan, K. W. (2010). Nigella sativa thymoquinone-rich fraction greatly improves plasma antioxidant capacity and expression of antioxidant genes in hypercholesterolemic rats. Free Radical Biology and Medicine, 48(5):664-672.
  23. Jacoeb, A. M., Nurjanah, Arifin, M., Sulistiono, W., & Kristiono, S. S. (2010). Deskripsi histologis dan perubahan komposisi kimia daun dan tangkai semanggi (Marsilea crenata Presl., Marsileaceae) akibat perebusan. Jurnal Pengolahan Hasil Perikanan Indonesia, 13(2):81-95.
  24. Jain, P. G., Patil, S. D., Haswani, N. G., Girase, M. V., & Surana, S. J. (2010). Hypolipidemic activity of Moringa oleifera Lam., Moringaceae, on high fat diet induced hyperlipidemia in albino rats. Revista Brasileira de Farmacognosia Brazilian Journal of Pharmacognosy, 20(6):969-973.
  25. Kurniawan, M., Izzati, M., & Nurchayati, Y. (2010). Kandungan klorofil, karotenoid, dan vitamin C pada beberapa spesies tumbuhan akuatik. Buletin Anatomi dan Fisiologi, 18(1):28-40.
  26. Lim, C. Y., Junit, S. M., Abdulla, M. A., & Aziz, A. A. (2013). In vivo biochemical and gene expression analyses of the antioxidant activities and hypocholesterolaemic properties of Tamarindus indica fruit pulp extract. Plos One, 8(7):e70058.
  27. Lutfiyati, I., Waznah, U., Slamet, S., & Wirasti, W. (2021). Uji Aktivitas antikolesterol partisi n-heksana, metanol dan ekstrak etanol kulit Jeruk Nipis (Citrus Aurantiifolia) secara in vitro. Paper presented at the Prosiding Seminar Nasional Kesehatan, Lembaga Penelitian dan Pengabdian Masyarakat, Universitas Muhammadiyah Pekajangan Pekalongan, Indonesia.
  28. Ma'arif, B., Agil, M., & Laswati, H. (2016). Phytochemical assessment on n-hexane extract and fractions of Marsilea crenata Presl. Leaves Through GC-MS. Traditional Medicine Journal, 21(2):77-85.
  29. Makatita, F. A., Wardhani, R., & Nuraini. (2020). Riset in silico dalam pengembangan sains di bidang pendidikan. Studi kasus: Analisis potensi cendana sebagai agen anti-aging. Jurnal ABDI (Sosial, Budaya dan Sains), 2(1):59-67.
  30. Masula, A. F., Puspitasari, D., Supriatin, S. W. E., Ummah, K., Rokhmatin, D., Mubarrok, M. M., Hariza, A. T., Isnawati, I., & Purnama, E. R. (2018). Docking molekuler senyawa metabolit sekunder Lantana camara sebagai antiinflamasi terhadap enzim Cox-1. Jurnal Biota, 4(2):79-83.
  31. Mirghaed, A. T., Hoseini S. M., Ghelichpour, M. (2018). Effects of dietary 1,8-cineole supplementation on physiological, immunological and antioxidant responses to crowding stress in rainbow trout (Oncorhynchus mykiss). Fish & Shellfish Immunology, 81:182-188.
  32. Muslim, T., Mentari, D. W., & Farhazakia, N. (2020). Daya dukung perairan rawa Mesangat sebagai habitat buaya Siam. Jurnal Ilmu Lingkungan, 18(3):436-445.
  33. Nwamarah, J. U., Otitoju, O., & Otitoju, G. T. O. (2015). Effects of Moringa oleifera Lam. aqueous leaf extracts on follicle stimulating hormone and serum cholesterol in Wistar rats. African Journal of Biotechnology, 14(3):181-186.
  34. Nurjanah, Azka, A., & Abdullah, A. (2012). Aktivitas antioksidan dan komponen bioaktif semanggi air (Marsilea crenata). Jurnal Inovasi dan Kewirausahaan, 1(3):152-158.
  35. Nursamsiar, Mangande, M. M., Awaluddin, A., Nur, S., & Asnawi, A. (2020). In silico study of aglycon curculigoside A and its derivatives as α-amilase inhibitors. IJPST (Indonesian Journal of Pharmaceutical Science and Technology), 7(1):29-37.
  36. Olorundare, O., Adeneye, A., Akinsola, A., Kolo, P., Agede, O., Soyemi, S., Mgbehoma, A., Okoye, I., Albrecht, R., & Mukhtar, H. (2020). Irvingia gabonensis seed extract: An effective attenuator of doxorubicin-mediated cardiotoxicity in Wistar rats. Oxidative Medicine and Cellular Longevity, 2020:1-14.
  37. Ouertani, A., Neifar, M., Ouertani, R., Masmoudi, A. S., & Cherif, A. (2019). Effectiveness of enzyme inhibitors in biomedicine and pharmacotherapy. Advances in Tissue Engineering and Regenerative Medicine, 5(3):85-90.
  38. Phatak, R. S., Khanwelkar, C. C., Matule, S. M., Datkhile, K. D., & Hendre, A. S. (2019). Antihyperlipidemic activity of Murraya koenigii leaves methanolic and aqueous extracts on serum lipid profile of high fat-fructose fed rats. Pharmacognosy Journal, 11(4):836-841.
  39. Pratiwi, D., Insanu, M., & Damayanti, S. (2016). Studi in silico senyawa antioksidan alami golongan steroid pada reseptor sistem reproduksi. Indonesia Natural Research Pharmaceutical Journal, 1(1):22-35.
  40. Rachmania, R. A., Supandi, & Larasati, O. A. (2015). Analisis in silico senyawa diterpenoid lakton herba Sambiloto (Andrographis paniculata Nees) pada reseptor alpha-glucosidase sebagai antidiabetes tipe II. Pharmacy, 12(02):210-222.
  41. Rahayuningsih, N., Sukmawan, Y. P., & Nurfatwa, M. (2018). Okra (Abelmoschus esculentus L. Moench) as anti-cholesterol, anti-diabetic and anti-obesity in white male rats. Paper presented at the International Conference on Pharmaceutical Research and Practice, Universitas Islam Indonesia, Sleman, Indonesia.
  42. Rahmayanti, A. N., Febriyanti, R. M., & Diantini, A. (2021). Review article: Antihyperlipidemic activity study of plants utilized by West Java society based on indigenous knowledge. Indonesian Journal of Biological Pharmacy, 1(1):33-39.
  43. Reddy, V. P., Urooj, A., Sairam, S., Ahmed, F., & Prasad, N. N. (2017). Hypocholesterolemic effect of Moringa oleifera polyphenols in rats fed high fat-cholesterol diet. Malaysian Journal of Nutrition, 23(2):473-478.
  44. Rinto, R., Dewanti, R., Yasni, S., & Suhartono, M. T. (2015). Isolasi dan identifikasi bakteri asam laktat penghasil inhibitor enzim HMG-KoA reduktase dari bekasam sebagai agen pereduksi kolesterol. Agritech, 35(3):309-314.
  45. Ruslin, Yana, N. R. A., & Leorita, M. (2020). Desain turunan senyawa leonurine sebagai kandidat obat anti inflamasi. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy), 6(1):181-191.
  46. Sahara, F. U., Slamet, S., Waznah, U., & Wirasti, W. (2021). Uji aktivitas antikolesterol ekstrak daun Puring (Codiaeum variegatum (L.) Rumph. Ex. A.Juss) secara in vitro. Paper presented at the Prosiding Seminar Nasional Kesehatan, Lembaga Penelitian dan Pengabdian Masyarakat, Universitas Muhammadiyah Pekajangan Pekalongan, Indonesia.
  47. Saleem, A., & Naureen, I. (2021). Effect of Moringa olifera on haematology and cholesterol level. Saudi Journal of Biomedical Research, 6(12):298-306.
  48. Saputri, K. E., Fakhmi, N., Kusumaningtyas, E., Priyatama, D., & Santoso, B. (2016). Docking molekular potensi anti diabetes melitus tipe 2 turunan Zerumbon sebagai inhibitor aldosa reduktase dengan Autodock-Vina. Chimica et Natura Acta, 4(1):16-20.
  49. Silalahi, M. (2020). Bioaktivitas asam jawa (Tamarindus indica) dan pemanfaatannya. Florea: Jurnal Biologi dan Pembelajarannya, 7(2):85-91.
  50. Suharta, S., Hunaefi, D., & Wijaya, C. H. (2021). Changes in volatile and aroma profile of Andaliman (Xanthoxylum acanthopodium DC.) upon various drying techniques. Food Chemistry, 365:130483.
  51. Sukandar, E. Y., Safitri, D., & Aini, N. N. (2016). The study of ethanolic extract of Binahong leaves (Andredera cordifolia (Ten.) Steenis) and Mulberry leaves (Morus nigra L.) in combination on hyperlipidemic induced rats. Asian Journal Pharmaceutical and Clinical Reearchs, 9(6):288-292.
  52. Sutrisna, E., Nuswantoro, Y., & Said, R. F. (2018). Hypolipidemic of ethanolic extract of Salam bark (Syzygium polyanthum (Wight) Walp.) from Indonesia (Preclinical study). Drug Invention Today, 10(1):55-58.
  53. Tibe, F., Rimpa, M., & Tandi, J. (2018). Uji efektivitas antikolesterol ekstrak etanol daun cincau hijau terhadap tikus putih jantan galur Wistar. Farmakologika Jurnal Farmasi, 15(2):134-141.
  54. Titisari, N., Fauzi, A., Adyana, A., & Trisunuwati, P. (2016). The effects of water clover (Marsilea crenata) extract against estrogen, progesterone and uterine histology on rat (Rattus norvegicus). International Journal of PharmTech Research, 9(6):165-171.
  55. Wardani, V. R., Fatimah, S., Nadia, & Cahyani, I. M. (2019). Aktivitas ekstrak etanol kulit melinjo (Gnetum gnemon L.) sebagai antikolesterol. Media Farmasi Indonesia, 14(1):1466-1470.
  56. Winthoko, E. N. V. A. P., Roosdiana, A., Pratama, D. A. O. A., Nugraha, J., Purwanta, M., Rifa'i, M. H., & Rendy, A. N. (2020). The potency of green grass jelly extract (Premna oblongifolia Merr) as antihyperlipidemia towards aorta histopathology representation of rat (Rattus norvegicus) induced with high fatty diet (HFD). Jurnal Teknologi Laboratorium, 9(1):97-102.
  57. Yang, M., Jin, Y., & Yang, L. (2018). A systematic summary of natural compounds in Radix glycyrrhizae. Traditional Medicine Research, 3(2):82-94.
  58. Yuniastuti, A. (2014). Peran pangan fungsional dalam meningkatkan derajat kesehatan. Prosiding Seminar Nasional & Internasional, 1-11.
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Author Biographies

Hardoko Hardoko, Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia

Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia

Syahrani Nurul Mutmainannah, Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia

Fisheries Products Technology Study Program, Department of Fishery Resources Management, Faculty of Fisheries and Marine Sciences, Brawijaya University, Malang, East Java, 65145. Indonesia

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