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Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Aquaculture

Feminization of Maroon Clownfish (Amphiprion biaculeatus, Bloch 1790) with 17β-Estradiol Hormonal Induction

https://doi.org/10.20473/jipk.v15i2.42765
Helena Sahusilawane
Post Graduate Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia; Aquaculture Study Program, Tual State Polytechnique of Fisheries, Tual, Maluku. Indonesia
https://orcid.org/0000-0002-7863-317X
Agus Oman Sudrajat
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0002-5125-3812
Muhmmad Agus Suprayudi
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia
https://orcid.org/0000-0003-1164-9502
Dinar Tri Soelistyowati
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia
Ligaya I. T. A. Tumbelaka
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia
Irzal Effendi
Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

Corresponding Author(s) : Agus Oman Sudrajat

agusom@apps.ipb.ac.id

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Highlight Research



  1. The 17β-estradiol hormone induction reduces the percentage of R values in skin and fin color by as much as 50-60%.

  2. The 17β-estradiol hormone induction increases total length and body weight, estradiol content, GIS and HIS, and also glucose content.

  3. The 17β-estradiol hormone induction shows mature development of the gonadal profile from functional male to functional female.


Abstract


 


As maroon clownfish (Amphiprion biaculeatus) is a protandrous hermaphroditic fish, feminization process with 17β-estradiol hormone can be applied to accelerate the female broodstock candidate supply for further spawning effort. This study aimed to evaluate the feminization of A. biaculeatus with 17β-estradiol hormonal induction. This study used a completely randomized design with several hormone dosage, namely P0 (without 17β-estradiol hormone induction), P1 (0.5 μg 17β-estradiol/g body), P2 (1.0 μg 17β-estradiol/g body), and P3 (1.5 μg 17β-estradiol/g body). These treatments were applied with three replications. Five fish composed of α-fish, β-fish, and three γ-fish were reared in each aquarium for 90 days with a flowing water system. The α- and β-fish were then removed, while the γ-fish was injected with hormone. Otohime pellet feed was fed three times a day until apparent satiation. The results showed that the 17β-estradiol hormone could induce 100% of the feminization process of male A. biaculeatus. The dosage of P3 obtained the lowest value percentage of red, green, blue (RGB), but showing the highest total of length and body weight (6.67±0.42 cm and 6.40±0.78 g, respectively), estradiol content (149.73±4.24 Ïg/mL), GSI and HSI (0.38±0.07% and 3.59±0.49%), and glucose content (4.67±0.64 mg/dL), followed by more mature gonad profile than other treatments. This condition indicates that fish in P3 treatment has been reversed as functional female. The average survival rate for the treatment was as high as 60%. Therefore, the application of 17β-estradiol hormonal induction is effective for the feminization process in A. biaculeatus as a protandrous hermaphroditic fish.

Keywords

Feminization 17β-estradiol A. biaculeatus Protandrous Hermaphroditic
Sahusilawane, H., Sudrajat, A. O., Suprayudi, M. A. ., Soelistyowati , D. T. ., Tumbelaka, L. I. T. A. ., & Effendi, I. (2023). Feminization of Maroon Clownfish (<I>Amphiprion biaculeatus<I/>, Bloch 1790) with 17β-Estradiol Hormonal Induction. Jurnal Ilmiah Perikanan Dan Kelautan, 15(2), 346–360. https://doi.org/10.20473/jipk.v15i2.42765
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Author Biographies

Helena Sahusilawane, Post Graduate Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia; Aquaculture Study Program, Tual State Polytechnique of Fisheries, Tual, Maluku. Indonesia

Post Graduate Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

Aquaculture Study Program, Tual State Polytechnique of Fisheries, Tual, Maluku. Indonesia

Agus Oman Sudrajat, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

Muhmmad Agus Suprayudi, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

Dinar Tri Soelistyowati , Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

Ligaya I. T. A. Tumbelaka, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

Irzal Effendi, Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

Department of Aquaculture, Faculty of Fisheries and Marine Sciences, IPB University, Bogor, West Java. Indonesia

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