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The Gonadal Maturity and Gene Expressions of Female Giant Freshwater Prawn (Macrobrachium rosenbergii) after Dietary Administration of Medroxyprogesterone Acetate
Corresponding Author(s) : Fajar Anggraeni
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
One of the problems in giant prawn cultivation is female giant prawns laying eggs during rearing. They will incubate their eggs for three weeks so that the energy from the expected feed for growth is used for egg development and other reproductive activities. Giving MPA hormone to giant prawns either by injection or oral could inhibit gonadal maturation and increase growth rate. The maturation process of the prawn ovary consists of the activation of some complex cellular mechanisms involving genes that regulate the stages of oocyte development. This study aimed to evaluate the response of gonadal maturity and the expression of the MrvWD-Kazal gene in giant prawns fed with a diet containing MPA hormone. The design used in this study was a completely randomized design with four treatments and five replications. The treatments were feeding a diet added with MPA with a concentration of 0 mg.kg-1 feed as control (K), 50 mg.kg-1 feed (P1), 100 mg.kg-1 feed (P2), and 150 mg.kg-1 feed (P3). MPA hormone at a concentration of 50-150 mg/kg feed could inhibit the gonadal maturation of female prawns. In 100 mg/kg of feed concentration showed the lowest gene expression level, indicating an inhibition of gonadal maturation molecularly. The administration of MPA hormone through the feed is a recommended method of aquaculture because it is more applicable and effective than by injection, even though it has a non-uniform impact on each individual.
Highlight Research
- Administration of MPA hormone through the feed to female giant freshwater prawns can inhibit the level of gonad maturity.
- Expression of von Willebrand factor D (vWD) – Kazal gene were analyzed in different levels of prawn maturity
- Gonadosomatic index and gonadal histology confirmed that different levels of administration of MPA hormone through the feed could affect the gonad development.
- The administration of MPA hormone through the feed is a recommended method for aquaculture because it is more applicable and effective to inhibit early maturation of prawns.
Keywords
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- Anggraeni, F., Malini, D. M., & Imron. (2021). Performa reproduksi dan pertumbuhan udang galah betina Macrobrachium rosenbergii setelah pemberian hormon medroxy progesterone acetat melalui pakan. Jurnal Riset Akuakultur, 16(2):83–91.
- Aprajita, K., Pandey, A. K.., Singh R., & Chauhan, U. K (2014). Reproductive endocrinal regulation in decapod crustaceans: A review. Journal of Ecophysiology and Occupational Health, 14(1–2):55–65.
- Baker, M. E. (1988). Invertebrate vitellogenin is homologous to human von Willebrand factor. Biochemical Journal, 256(3):1059–1061.
- Cavallo, R. O., Lavens, P., & Sorgeloos, P. (2001). Reproductive performance of Macrobrachium rosenbergii females in capacity. Journal of the World Aquaculture Society, 32(1):60–67.
- Chang, C. F., & Shih, T. W. (1995). Reproductive cycle of ovarian development and vitellogenin profiles in the freshwater prawns, Macrobrachium rosenbergii. Invertebrate Reproduction & Development, 27(1):11–20.
- Dall, W., Hill, B. J., Rothlisberg, P. C., & Staples, D. J., (1990). Biology of the Penaeidae. In J. H. S. Blaxter, A. J. Southward (Ed.), Advances in marine biology. (pp. 489). London: Academic Press.
- da Silva, G. M. F., Ferreira, M. A. P., von Ledebur, E. I. C. F., & da Rocha, R. M. (2009). Gonadal structure analysis of Macrobrachium amazonicum (Heller, 1862) from a wild population: A new insight into the morphotype characterization. Aquaculture Research, 40(7):798–803.
- Effendie, M. I. (1979). Metode biologi perikanan. Bogor: Yayasan Dewi Sri.
- Faiz, Z. M., Mardhiyyah, M. P., Mohamad, A., Hidir, A., Nurul-Hidayah, A., Wong, L., Jasmani, S., & Ikhwanuddin, M. (2019). Identification and relative abundances of mRNA for a gene encoding the vWD domain and three Kazal-type domains in the ovary of giant freshwater prawns, Macrobrachium rosenbergii. Animal Reproduction Science, 209:1–9.
- Fatihah, S. N., Hassan, A. R., Fadhilah, N., Harman, M. F., & Ikhwanudin, M. (2022). Gonad maturation of giant freshwater prawn, Macrobrahium rosenbergii at the bay of Padas River, Beaufort, Sabah, Malaysia. Transactions on Science and Technology, 9(4):177–184.
- Finn, R. N. (2007). Vertebrate yolk complexes and the functional implications of Phosvitins and other subdomains in Vitellogenins1. Biology of Reproduction, 76(6):926–935.
- Khasani, I., Krettiawan, H., Sopian, A., & Anggraeni, F. (2018). Selection response and heritability of growth traits of giant freshwater prawn (Macrobrachium rosenbergii) in Indonesia. AACL Bioflux, 11(6):1688–1695.
- Laining, A., Lante, S., & Usman, U. (2015). Induksi pematangan gonad dan peningkatan tingkat pembuahan telur induk udang windu, Penaeus monodon melalui rangsangan hormonal tanpa ablasi mata. Jurnal Riset Akuakultur, 10(1):61.
- Livak, K. J., & Schmittgen, T. D., (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆cT method. Methods, 25(4):402–408.
- Merlin, J., Mohanlal, D. L., Balasubramanian, C. P., Sherly, T., Subramoniam, T., Syamadayal, J., Ravichandran, P., Ponniah, A. G., Gopal, C., & Vijayan, K. K. (2015). Induction of vitellogenesis and reproductive maturation in tiger shrimp, Penaeus monodon by 17β-estradiol and 17α- hydroxyprogesterone: in vivo and in vitro studies. Invertebrate Reproduction & Development, 59(3):166–175.
- Mohamad, A., Arshad, A., Sung, Y. Y., & Jasmani, S. (2017). Effect of thermal stress on Hsp70 gene expression and female reproductive performance of giant freshwater prawn, Macrobrachium rosenbergii. Aquaculture Research, 49(1):135–150.
- Nagaraju, G. P. C. (2011). Reproductive regulators in decapods crustacean: An overview. The Journal of Experimental Biology, 214(1):3–16.
- Okumura, T. (2004). Perspectives on hormonal manipulation of shrimp reproduction. Japan Agricultural Research Quarterly, 38(1):49–54.
- Qiu, G. F., & Yamano, K. (2005). Three forms of cyclin B transcripts in the ovary of the kuruma prawn Marsupenaeus japonicus: Their molecular characterizations and expression profiles during oogenesis. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 141(2):186–195.
- Parawangsa, I. N. Y., Artha, G. A. K., & Tampubolon, P. A. (2022). Morphoregression and reproduction aspect of bonylip barb (Osteochilus vitattus Valenciennes, 1842) in Tamblingan Lake, Bali Island. Jurnal Ilmiah Perikanan dan Kelautan, 14(2):272–284.
- Reddy, C. S., Reddy, D. C., & Kalarani, V. (2013). Changes in the ovarian vitellogenin levels during ovarian cycle in the giant freshwater prawn, Macrobrachium rosenbergii. Weekly Science International Research Journal, 1:1–15.
- Tata, J. R., & Smith, D. F. (1979). A Versatile Model for Hormonal Regulation of Gene Expression. Proceeding of the 1978 Laurentian Hormone Conference, 35:47–95.
- Tave, D. (1995). Selective breeding programmes for medium-sized fish farms. Rome: FAO.
- Wijaya, M., Sudrajat, A. O., & Imron. (2020). Reproductive and growth performances in female giant freshwater prawn following inhibition of gonadal maturation using dopamine and medroxyprogesterone hormone. Jurnal Akuakultur Indonesia, 19(1):10–18.
- Zhang, F., Chen, L., Wu, P., Zhao, W., Li, E., & Qin, J. (2010). cDNA cloning and expression of Ubc9 in the developing embryo and ovary of oriental river prawn, Macrobrachium nipponense. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 155(3):288–293.
- Zhang, F., Chen, L. Zhao, W., & Wu, P. (2011a). A novel gene with a vWD domain and three Kazal-type domains: molecular cloning and expression in the ovary of the oriental river prawn, Macrobrachium nipponense. Genetika, 47(9):1190–1195.
- Zhang, F., Chen, L., Qin, J., Zhao, W., Wu, P., Yu, N., & Ma, L. (2011b). cDNA cloning and expression analysis of Gustavus gene in the oriental river prawn Macrobrachium nipponense. PLoS ONE, 6(2)1–7.
- Zhang, F. Chen, L., Zhao, W., Wu, P., Li, E., Yu, N., Qin, J., & Ma, L. (2016). Two genes with fertile attributes from Macrobrachium nipponense (De Haan, 1849) (Natantia: Palaemonidae): Evidence from expression analysis of Mago nashi and Tsunagi proteins during oocyte maturation and embryonic development. Journal of Crustacean Biology, 36(2):229–237.
- Zhao, Y., Castiglioni, S., & Fent, K. (2015). synthetic progestins medroxyprogesterone acetate and dydrogesterone and their binary mixtures adversely affect reproduction and lead to histological and transcriptional alterations in zebra fish (Danio rerio). Environmental Science & Technology, 49(7):4636–4645.
References
Anggraeni, F., Malini, D. M., & Imron. (2021). Performa reproduksi dan pertumbuhan udang galah betina Macrobrachium rosenbergii setelah pemberian hormon medroxy progesterone acetat melalui pakan. Jurnal Riset Akuakultur, 16(2):83–91.
Aprajita, K., Pandey, A. K.., Singh R., & Chauhan, U. K (2014). Reproductive endocrinal regulation in decapod crustaceans: A review. Journal of Ecophysiology and Occupational Health, 14(1–2):55–65.
Baker, M. E. (1988). Invertebrate vitellogenin is homologous to human von Willebrand factor. Biochemical Journal, 256(3):1059–1061.
Cavallo, R. O., Lavens, P., & Sorgeloos, P. (2001). Reproductive performance of Macrobrachium rosenbergii females in capacity. Journal of the World Aquaculture Society, 32(1):60–67.
Chang, C. F., & Shih, T. W. (1995). Reproductive cycle of ovarian development and vitellogenin profiles in the freshwater prawns, Macrobrachium rosenbergii. Invertebrate Reproduction & Development, 27(1):11–20.
Dall, W., Hill, B. J., Rothlisberg, P. C., & Staples, D. J., (1990). Biology of the Penaeidae. In J. H. S. Blaxter, A. J. Southward (Ed.), Advances in marine biology. (pp. 489). London: Academic Press.
da Silva, G. M. F., Ferreira, M. A. P., von Ledebur, E. I. C. F., & da Rocha, R. M. (2009). Gonadal structure analysis of Macrobrachium amazonicum (Heller, 1862) from a wild population: A new insight into the morphotype characterization. Aquaculture Research, 40(7):798–803.
Effendie, M. I. (1979). Metode biologi perikanan. Bogor: Yayasan Dewi Sri.
Faiz, Z. M., Mardhiyyah, M. P., Mohamad, A., Hidir, A., Nurul-Hidayah, A., Wong, L., Jasmani, S., & Ikhwanuddin, M. (2019). Identification and relative abundances of mRNA for a gene encoding the vWD domain and three Kazal-type domains in the ovary of giant freshwater prawns, Macrobrachium rosenbergii. Animal Reproduction Science, 209:1–9.
Fatihah, S. N., Hassan, A. R., Fadhilah, N., Harman, M. F., & Ikhwanudin, M. (2022). Gonad maturation of giant freshwater prawn, Macrobrahium rosenbergii at the bay of Padas River, Beaufort, Sabah, Malaysia. Transactions on Science and Technology, 9(4):177–184.
Finn, R. N. (2007). Vertebrate yolk complexes and the functional implications of Phosvitins and other subdomains in Vitellogenins1. Biology of Reproduction, 76(6):926–935.
Khasani, I., Krettiawan, H., Sopian, A., & Anggraeni, F. (2018). Selection response and heritability of growth traits of giant freshwater prawn (Macrobrachium rosenbergii) in Indonesia. AACL Bioflux, 11(6):1688–1695.
Laining, A., Lante, S., & Usman, U. (2015). Induksi pematangan gonad dan peningkatan tingkat pembuahan telur induk udang windu, Penaeus monodon melalui rangsangan hormonal tanpa ablasi mata. Jurnal Riset Akuakultur, 10(1):61.
Livak, K. J., & Schmittgen, T. D., (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2-∆∆cT method. Methods, 25(4):402–408.
Merlin, J., Mohanlal, D. L., Balasubramanian, C. P., Sherly, T., Subramoniam, T., Syamadayal, J., Ravichandran, P., Ponniah, A. G., Gopal, C., & Vijayan, K. K. (2015). Induction of vitellogenesis and reproductive maturation in tiger shrimp, Penaeus monodon by 17β-estradiol and 17α- hydroxyprogesterone: in vivo and in vitro studies. Invertebrate Reproduction & Development, 59(3):166–175.
Mohamad, A., Arshad, A., Sung, Y. Y., & Jasmani, S. (2017). Effect of thermal stress on Hsp70 gene expression and female reproductive performance of giant freshwater prawn, Macrobrachium rosenbergii. Aquaculture Research, 49(1):135–150.
Nagaraju, G. P. C. (2011). Reproductive regulators in decapods crustacean: An overview. The Journal of Experimental Biology, 214(1):3–16.
Okumura, T. (2004). Perspectives on hormonal manipulation of shrimp reproduction. Japan Agricultural Research Quarterly, 38(1):49–54.
Qiu, G. F., & Yamano, K. (2005). Three forms of cyclin B transcripts in the ovary of the kuruma prawn Marsupenaeus japonicus: Their molecular characterizations and expression profiles during oogenesis. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 141(2):186–195.
Parawangsa, I. N. Y., Artha, G. A. K., & Tampubolon, P. A. (2022). Morphoregression and reproduction aspect of bonylip barb (Osteochilus vitattus Valenciennes, 1842) in Tamblingan Lake, Bali Island. Jurnal Ilmiah Perikanan dan Kelautan, 14(2):272–284.
Reddy, C. S., Reddy, D. C., & Kalarani, V. (2013). Changes in the ovarian vitellogenin levels during ovarian cycle in the giant freshwater prawn, Macrobrachium rosenbergii. Weekly Science International Research Journal, 1:1–15.
Tata, J. R., & Smith, D. F. (1979). A Versatile Model for Hormonal Regulation of Gene Expression. Proceeding of the 1978 Laurentian Hormone Conference, 35:47–95.
Tave, D. (1995). Selective breeding programmes for medium-sized fish farms. Rome: FAO.
Wijaya, M., Sudrajat, A. O., & Imron. (2020). Reproductive and growth performances in female giant freshwater prawn following inhibition of gonadal maturation using dopamine and medroxyprogesterone hormone. Jurnal Akuakultur Indonesia, 19(1):10–18.
Zhang, F., Chen, L., Wu, P., Zhao, W., Li, E., & Qin, J. (2010). cDNA cloning and expression of Ubc9 in the developing embryo and ovary of oriental river prawn, Macrobrachium nipponense. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 155(3):288–293.
Zhang, F., Chen, L. Zhao, W., & Wu, P. (2011a). A novel gene with a vWD domain and three Kazal-type domains: molecular cloning and expression in the ovary of the oriental river prawn, Macrobrachium nipponense. Genetika, 47(9):1190–1195.
Zhang, F., Chen, L., Qin, J., Zhao, W., Wu, P., Yu, N., & Ma, L. (2011b). cDNA cloning and expression analysis of Gustavus gene in the oriental river prawn Macrobrachium nipponense. PLoS ONE, 6(2)1–7.
Zhang, F. Chen, L., Zhao, W., Wu, P., Li, E., Yu, N., Qin, J., & Ma, L. (2016). Two genes with fertile attributes from Macrobrachium nipponense (De Haan, 1849) (Natantia: Palaemonidae): Evidence from expression analysis of Mago nashi and Tsunagi proteins during oocyte maturation and embryonic development. Journal of Crustacean Biology, 36(2):229–237.
Zhao, Y., Castiglioni, S., & Fent, K. (2015). synthetic progestins medroxyprogesterone acetate and dydrogesterone and their binary mixtures adversely affect reproduction and lead to histological and transcriptional alterations in zebra fish (Danio rerio). Environmental Science & Technology, 49(7):4636–4645.