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Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Molecular Genetics

DNA Barcoding of Red Algae (Rhodophyta) in Ternate Island Sea, North Maluku, Indonesia

https://doi.org/10.20473/jipk.v16i1.44436
Muhammad Janib Achmad
Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia
https://orcid.org/0000-0001-7216-9970
Nebuchadnezzar Akbar
Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia
Firdaut Ismail
Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia
Ardan Samman
Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia
Beginer Subhan
Department of Marine Science and Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, West Java, 16680. Indonesia
Rustam E Paembonan
Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia
Dondy Arafat
Department of Marine Science and Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, West Java, 16680. Indonesia

Corresponding Author(s) : Muhammad Janib Achmad

mjachmad18@gmail.com

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract


Abstract


Ternate Island is located on the North Maluku Sea. The North Maluku Sea region includes the Wallacea area and the world's coral triangle. One of the organisms commonly found in this sea is red algae. Study aimed to determine the red algae species by phylogenetic tree analysis based on the rbcL gene as a DNA marker. The preserved red algae tissue samples were extracted with the Geneaid GP100 DNA Extraction Kit Plant. The DNA sample was amplified and then visualized by 1% agarose gel electrophoresis. The amplicon products were sequenced and then aligned with the rbcL gene database that was available at the NCBI gene bank. The phylogenetic tree was constructed using the UPGMA method. The results showed that red algae were identified into four species: namely Gibsmithia hawaiiensis (98.65%), C_rbcL sample was identical to Amansieae sp. (91.50%), D_rbcL sample was identical to Peyssonnelia sp. (95.54%), and G_rbcL sample was similar to Portieria hornemanniI (96.15%). Based on phylogenetic tree analysis, Gibsmithia hawaiiensis from North Maluku is closely related to species from Raja Ampat, West Papua, Indonesia, followed by the Philippines species. Special findings were found to carry out phylogenetic reconstructions that can answer inter-species kinship. The general finding is that the markers used can be used for phylogenetic construction. Phylogenetic construction of Peyssonnelia sp. in North Maluku is related to species from South Africa. North Maluku's Portieria hornemannii is closely related to a species from Korea.


Highlight Research



  1. This study provides initial information about the DNA barcoding of red algae.

  2. Important for management purposes and determining the status of red algae in the future.

  3. This scientific information also provides an understanding of the relationships among red algae species in the world's oceans.

  4. Global warming has changed the temperature of seawater and affected the population of marine organisms.

Keywords

Biodiversity Distribution Locus Marine Organisms Molecular
Achmad, M. J., Akbar, N. ., Ismail, F. ., Samman, A., Subhan, B. ., E Paembonan, R., & Arafat, D. (2023). DNA Barcoding of Red Algae (Rhodophyta) in Ternate Island Sea, North Maluku, Indonesia. Jurnal Ilmiah Perikanan Dan Kelautan, 16(1), 234–244. https://doi.org/10.20473/jipk.v16i1.44436
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Author Biographies

Muhammad Janib Achmad, Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia

Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia

Nebuchadnezzar Akbar, Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia

Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia

Firdaut Ismail, Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia

Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia

Ardan Samman, Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia

Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia

Beginer Subhan, Department of Marine Science and Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, West Java, 16680. Indonesia

Department of Marine Science and Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, West Java, 16680. Indonesia

Rustam E Paembonan, Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia

Faculty of Fisheries and Marine Science, Khairun University, Gambesi Campus, Ternate, North Maluku, 97716. Indonesia

Dondy Arafat, Department of Marine Science and Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, West Java, 16680. Indonesia

Department of Marine Science and Technology, Faculty of Fisheries and Marine Sciences, Bogor Agricultural University, Bogor, West Java, 16680. Indonesia

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