PENGARUH PEMBERIAN INSULIN-LIKE GROWTH FACTOR-I (IGF-I) DARI SERUM KUDA CROSSBREED BUNTING TERHADAP KETEBALAN ENDOMETRIUM MENCIT (Mus musculus)

Dyah Ayu Roro Risna Y, Sri Mulyati, Roesno Darsono, Imam Mustofa, Arimbi Arimbi, Widjiati Widjiati

= http://dx.doi.org/10.20473/ovz.v7i2.2018.120-125
Abstract views = 230 times | downloads = 272 times

Abstract


The purpose of the research was to know  the effect of Insulin-Like Growth Factor-I (IGFI) derived from pregnant crossbreed mare serum (PMS) on endometrium thickness of mice (Mus musculus). The subject of this research were 35 female mice. The research was arranged by Completely Randomized Design (CRD) with seven treatment and five replications. The treatment were P0 = 10 ng/ml of physiological NaCl, P11 = 10 ng/ml of IGF-I PMS, P12 = 20 ng/ml of IGF-I PMS, P13 = 40 ng/ml of IGF-I PMS, P21 = 10 ng/ml of IGF-I recombinant mouse, P22 = 20 ng/ml of IGF-I recombinant mouse, and P23 = 40 ng/ml of IGF-I recombinant mouse. Observed variables include histopatological endometrium thickness of mice. The data were analyzed by Analysis of Variance (ANOVA), followed by HSD (Honestly Significant Difference) test. The data was also be analyzed using General Linear Model Univarieted to see the comparison between IGF-I PMS and recombinant mouse. The result showed that the addition of IGF-I PMS did not significantly affect (p>0,05) on endometrium thickness of mice . It showed that did not significantly difference (p>0,05) between the effect of IGF-I PMS and IGF-I recombinant mouse against the endometrium thickness of mice.

Keywords


IGF-I, IGF-I pregnant crossbreed mare serum, endometrium thickness, Mus musculus.

Full Text:

PDF

References


Acahe, H., A. Revel. 2006. Endometrial Receptivity Markers, The Journey To Succesfull Embryo Implantation. Tachtech Edical Journey. Bearden, H., Joe., J.W. Fuquay, and S.T. Willard. 2004. Applied Animal Reproduction. Upper Saddle River. New Jersey. Brzozowski, AM., E.J Dodson., GG Dosdon. 2002. Structural Origin Of The Functional Divergence Of Human Insulin-Like Growth Factor-I And Insulin. Biochemistry. Douglas W. Cromey, M.S. 2004. Formaldehyde fixtative. SWEHSC Cellu-lar Imaging Core. Ganiswara. 1995. Farmakologi dan Terapi Edisi 4. Gaya Baru: Jakarta. Inoue, Ayako., Takeuchi, Sakae, and T. Sumio. 2005. Insulin-Like Growth Factor-I Stimulated DNA Replication in Mouse Endometrial Stroma Cells. Journal of Reproduction and Development. 51: 3. Ismudiono, Srianto, P., Anwar, H., Madyawati, S.P., Samik, A., dan Safitri E. 2010. Buku Ajar Fisiologi Reproduksi Pada Ternak. Airlangga University Press. Surabaya. Klotz D.M., S.C. Hewitt, P. Ciana, M. Raviscioni, J. K. Lindezy, J. Foley, A. Maggi, R.P. Diagustine, and K.S. Kroach. 2001. Requirement of Estrogen Receptor-α In Insulin-like Growth Factor-I (IGF-I)-indunced Uterine Responses and in Vivo Evidence for IGF-I/Estrogen Receptor Cross-talk. J. Bio. Chem. 277: 8531-8537. Muchsin, R. 2009. Pengaruh Pemberian Monosodium Glutamate terhadap Histologi Endometrium Mencit (Mus musculus l) [Tesis]. Sekolah Pascasarjana. Universitas Sumatera Utara Medan. Murphy, L.J., Ghahary A. 1990. Uterine Insulin-Like Growth Factor-1: Regulation of Expression and Its Role in Estrogen-Induced Uterine Proliferation. Endocrinology Rev. 11(3): 443-453. Oner, J., Oner, H. 2007. Immunolocalization of Insuline-Like Growth Fac-tor I (IGF-I) During Preimplatation in Rat Uterus. Growth Hormone and IGF-1 research. 271-278.

Peters, A.R. 1985. Hormonal Control of Bovine Oestrus Cycle I. The Natural Cycle. Br. Vet. J. Reprod. Fertil. 47: 1-114 Roche, J.F. 1998. Controlls Of Foliculogenesis. J Theriology. 49: 457-467. Restiadi, T.I. 2013. Potensi Biologis Bahan Bioaktif Insulin-Like Growth Factor-I Asal Serum Kuda Crossbreed G3-G4 Bunting pada Fertilisasi In Vitro Pada Sapi [Desertasi]. Fakul-tas Kedokteran Hewan. Universitas Airlangga. Surabaya. Sulisdiani, P. 2016. Pengaruh Pemberian Insuline-Like Growth Factor-1 dari Serum Kuda Crossbreed Bunting terhadap Siklus Estrus Mencit (Mus musculus) [Skripsi]. Fakultas Kedokteran Hewan. Universitas Airlangga. Surabaya.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Dyah Ayu Roro Risna Y, Sri Mulyati, Roesno Darsono, Imam Mustofa, Arimbi Arimbi, Widjiati Widjiati

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

View My Stats

Creative Commons License
Ovozoa: Journal of Animal Reproduction by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

      

     

    

 

This journal is supported by