Kualitas spermatozoa post-thawing pejantan sapi Friesian Holstein pada umur yang berbeda

Widya Aulia Ristiani, Muhammad Yunus, Tri Wahyu Suprayogi, Pudji Srianto, Imam Mustofa, Rimayanti Rimayanti

= http://dx.doi.org/10.20473/ovz.v9i1.2020.12-16
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Abstract


This study aimed to determine the post-thawing spermatozoa quality of Holstein-Friesian (HF) bulls at different ages. The samples were HF frozen semen of 3, 5, and 8-years -old age group produced by the Singosari National Artificial Insemination Center.  Nine samples of each group were randomly purposive sampling, thawed in 37ºC water for 15 seconds, and assessed on the percentage of viability, motility, and abnormality of spermatozoa. The obtained data were analyzed using the ANOVA method, followed by Duncan's multiple range test at a 95% confidence level. The result showed that post-thawing spermatozoa viability in 5 and 8-year-old HF bull were 67 ± 6.67%, and 78 ± 7.59, both were higher (p <0.05) compared to 62.78 ± 5.76% in HF bulls 3-yearsold group. Likewise, the motility of those bull aged 5 and 8 years (69.44 ± 6.82 and 65.00 ± 9.68%, respectively) were higher (p <0.05) compared to 55, 56 ± 5.83% in 3-year-old HF bull. Meanwhile, the percentage of post-thawing spermatozoa abnormalities in the three age groups were 4.33 ± 1.96%, 5.89 ± 1.90%, and 5.33 ± 1.41%, not significantly different (p> 0.05) respectively. It concluded that the best quality of post-thawing HF semen based on the percentage of viability, motility, and morphologic abnormality was collected from 5-8 years old bull. Nevertheless, spermatozoa from the three age goups still meet the Indonesia standard quality of frozen semen for artificial insemination.

Keywords


Holstein-Friesian bull, sperm quality, post thawing, different ages

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Azzahra FT, Setiatin ET, Samsudewa D. 2016. Evaluasi motilitas dan presentase hidup semen segar Sapi PO Kebumen pejantan muda. Fakultas Peternakan dan Pertanian Universitas Diponegoro Semarang. J Sains Peternakan Indonesia 2: 99-107. Badan Standarisasi Nasional Indonesia (BNSI) 2017. SNI (Standar Nasional Indonesia) 4869-1. Semen beku - Bagian 1: Sapi. Jakarta, Indonesia. Barth AD, Oko RJ. 1989. Abnormal morphology of bovine spermatozoa. Iowa State University Press. Iowa. Butarbutar E. 2009. Efektifitas frekuensi exercise terhadap peningkatan kualitas semen sapi Simmental. Skripsi. Fakultas Pertanian universitas Sumatra Utara. 23-50. Garner DL, Hafez ESE. 2008. Spermatozoa and Seminal Plasma. Dalam: Hafez ESE (editor). Reproduction in Farm Animmal. Edisi 7. Lea and Febiger: 96-110. Ismaya. 2014. Bioteknologi inseminasi buatan pada sapi dan kerbau. Yogyakarta: Gadjah Mada University Press. 12. Isnaini N, Wahjuningsih S, Ma’ruf A, Witayanto DA. 2019. Effects of age and breed on semen quality of beef bull sires in an Indonesian artificial insemination center. Livestock Research for Rural Development. http://www.lrrd.org/lrrd31/5/nurul31078.ht ml. Kusriningrum. 2009. Buku ajar perancangan percobaan. Dani Abadi. Surabaya. 31. Lestari S, Saleh DM, Maidaswar M. 2013. Profil kualitas semen segar sapi pejantan Limousin dengan umur yang berbeda di Balai Inseminasi Buatan Lembang Jawa Barat. J Ilmu Peternakan. 1: 1165-72. Medeiros CMO, Forell F, Oliveira ATD, Rodrigues JL. 2002. Current status of sperm cryopreservation: why isn't it better? Theriogenology 57: 327–44 Melita D, Dasrul D, Adam M. 2014. Pengaruh umur pejantan dan frekuensi ejakulasi terhadap kualitas spermatozoa sapi Aceh. J Medika Veterinaria 8: 15-9. Menon AG, Barkema HW, Wilde R, Kastelic JP, Thundathil JC. 2011. Associations between sperm abnormalities, breed, age, and scrotal circumference in beef bulls. Can J Vet Res. 75: 241‐247. Murphy EM, Kelly AK, O'Meara C, Eivers B, Lonergan P, Fair S. 2018. Influence of bull age, ejaculate number, and season of collection on semen production and sperm motility parameters in Holstein Friesian bulls in a commercial artificial insemination centre. J Anim Sci. 96:

‐18. Nyuwita AT, Susilawati T, Isnaini N. 2015. Kualitas semen segar dan produksi semen beku sapi Simmental pada umur yang berbeda. Ternak Tropika 16: 1-8 Purwantara B, Arifiantini RI, Riyadhi M. 2010. Sperm morphological assessments of FH bull semen collected from three Artificial Insemination Centers in Indonesia. J Indonesian Trop. Anim. Agric. 35: 90-4. Salmah N. 2014. Motilitas, presentase hidup dan abnormalitas spermatozoa semen beku sapi Bali pada pengenceran andromed dan tris kuning telur. Skripsi. Fakultas Peternakan Universitas Hasanuddin. Makassar. 37-8. Sankhi S, Sapkota KR, Regmi B. 2018. Effect of age and frequency of collection in quality of Jersey bulls semen at National Livestock Breeding Center, Nepal. Nepalese Vet J. 35: 9-22. Sardjito T. 2014. Identifikasi kasus infertilitas dan pola pita protein gel SDS PAGE serum sapi betina Crossbreed Baluran dan perubahan molekuler DNA pada semen pejantan baluran post thawing. Laporan Penelitian Universitas Airlangga. Susilawati T, Suyadi N, Isnaini N, Wahyuningsih S. 1993. Kualitas semen sapi Fries Holland dan sapi Bali pada berbagai umur dan berat badan. Laporan Penelitian. Fakultas Peternakan Universitas Brawijaya, Malang. Susilowati S, Hardijanto H, Suprayogi TW, Sardjito T, Hernawati T. 2010. Petunjuk praktikum inseminasi buatan. Airlangga University Press. Surabaya. 5-37. Varasofiari LN, Setiatin ET, Sutopo S. 2013. Evaluasi kualitas semen segar sapi Jawa Brebes berdasarkan lama waktu penyimpanan. Fakultas Peternakan dan Pertanian Universitas Diponegoro Semarang. Animal Agriculture 1: 201-108. Waberski D. 2018. Artificial insemination in domestic and wild animal species. Dalam: Niemann H, Wrenzycki C (editor). Animal biotechnology 1. Springer International Publishing AG part of Springer Nature. 3764.


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