EFEK PEMBERIAN L-ARGININ TERHADAP GAMBARAN HISTOLOGI JUMLAH SPERMATOSIT PRIMER PADA MENCIT (Mus musculus) SETELAH TERPAPAR SUHU PANAS

Dirga Januar Surya Utama, Suherni Susilowati, Tatik Hernawati, Erma safitri, Sri Mulyati, Tri Nurhajati

= http://dx.doi.org/10.20473/ovz.v8i1.2019.22-25
Abstract views = 252 times | downloads = 1355 times

Abstract


The purpose of this study was to know the effect of L-arginine on the histology of primary spermatocyte count in mice (Mus musculus) after high temperature exposed. The subjects of this study were 20 adult male mice, 8 weeks old with an body weight range from 20-40 grams. This research conducted by using Complete Randomized Design (RAL) with 4 treatments and 5 replications. The treatments consisted of, P0- = treatment with 1ml of aquabidest without high temperature exposed, P0 + = treatment with 1ml of aquabidest after high temperature exposed for 1hour per-day for 35 days, P1 = 1.3mg / day L-arginine dissolved in 1ml aquabidest and given orally after high temperature exposed for 1hour per-day for 35 days and P2 = 2.6mg / day L-arginine dissolved in 1ml aquabidest given orally after high temperature exposed for 1hour per-day for 35 days. Observations done by making histologic preparations of testicular organs and then calculated the total number of primary spermatocyte per treatment. The data of primary spermatocyte calculated and analyzed by using Analysis of Variance (ANOVA), followed by Duncan test. The result from data analysis showed that there was a significant difference (p<0.05) between P1 and P2 with control,between P1 and P2 the analysis did not show any significant  difference (p> 0.05).

Keywords


L-arginine, primary spermatocyte, Mus musculus

Full Text:

PDF

References


Al-Ebady, A, S., S. O. Hussain., K. I. AlBadry., A. B. Rajab. 2012. Effect of ArginineinDifferent Concentrations on Some Physical Properties of Poor Motile Bull Sperms During Different Months. Saudi Arabia and Iraq, Journal of Veterinary Medicine and Animal Health. 4(9):130-135. Appleton, J. N. 2002. Arginine: Clinical Potential of a Semi-Essential Amino Acid. Alternative Medicine Review. Dada, R., N. P. Gupta., and K. Kuncheria. 2001. Deterioration of Sperm Morphology in Men Exposed to High Temperature. J Anat. Soc. 50(2) 107111. Doshi, S. B. 2012. Role of Reactive Nitrogen Species in Male Infertility. Reproductive Biologyand Endocrinology. Ermiza. 2012. Pengaruh Paparan Suhu Terhadap Kualitas Spermatozoa Mencit Jantan (Mus musculus) strain jepang. Sainstis. 19-28. Hafez, E. S. E. 2000. Reproduksi in Farm Animals. 7th Ed. Philadelphia : Lippincott Williams and Wilkins. Hariadi, M., S. Hardjopranyoto., Wurlina., H. A. Hermadi., B. Utomo,, Rimayanti., I. N. Triana., H. Ratnani. 2011. Buku Ajar Ilmu Kemajiran pada Ternak. Surabaya: Airlangga University Press. Hermadi, H, A. 2015. Pemberantasan Kasus Kemajiran pada Ternak Menuju Kemandirian Dibidang Kesehatan Reproduksi Hewan dan Ketahanan Pangan Di Indonesia. Perpustakaan Universitas Airlangga. Husein, R, H., M. O. Ahmed., S. M. Muhammed. 2011. Effect of LArginine, Vitamin E and Their Combination on Sperms Morphology in Albino Male Mice. Journal of AlNahraim University. 14: 137-145. Ismudiono., P. Srianto., H. Anwar., S. P.Madyawati., A. Samik., E. Safitri. 2010. Buku Ajar Fisiologi Reproduksi padaTernak. Surabaya. Airlangga University Press.

Khaira, K. 2010. Menangkal Radikal Bebas dengan Anti-Oksidan. Jurnal Sainstek. II(2): 183-187. Kusriningrum, R. S. 2008. Perancangan Percobaan. Surabaya: Airlangga University Press. McCann, S. M. 1999. The Role of Nitric Oxide in Reproduction. Brazilian Journal of Medical and Biological Research. 32(11):1367-1379. Morris, J. S. 2016. Arginine : beyond protein. The American Journal of Clinical Nutrition. 1(1): 17-27. Pineda, M. H. and M. P. Dooley.2003. McDonald's Veterinary Endocrinology and Reproduction. vol. 5 thed. Iowa State Press. Sukardi, S. 2006. Effects of L-Arginine on the Reproductive System of Male Rabbits. Mal J Nutr. 12(2): 201-211.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Dirga Januar Surya Utama, Suherni Susilowati, Tatik Hernawati, Erma safitri, Sri Mulyati, Tri Nurhajati

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

View My Stats

Creative Commons License
Ovozoa: Journal of Animal Reproduction by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

      

     

    

 

This journal is supported by