PENGARUH PENAMBAHAN EKSTRAK TEH HIJAU (Camellia sinensis) DALAM BAHAN PENGENCER SUSU SKIM KUNING TELUR TERHADAP KUALITAS SPERMATOZOA DOMBA SAPUDI YANG DISIMPAN PADA SUHU DINGIN

Wahyu Retno Swari, Emy Koestanti Sabdoningrum, Wurlina Wurlina, Suherni Susilowati, Rochmah Kurnijasanti, Erma Safitri

= http://dx.doi.org/10.20473/ovz.v8i2.2019.122-126
Abstract views = 538 times | downloads = 1041 times

Abstract


The purpose of this study was to determinated the effect and the best concentration of greentea extract in skim milk and egg yolk diluent for quality sapudi sheep spermatozoa were measured in motility, viability and intact plasma membrane that was stored on cold temperature. The semen was devided into four groups; skim milk and egg yolk diluent, 0,5% green tea extract in skim milk and egg yolk diluent, 0,1% green tea extract in skim milk and egg yolk diluent, and 0,15% green tea extract in skim milk and egg yolk diluent. Spermatozoa quality was observed day 1, day 2, day 3, day 4 and day 5 after being diluted. The data obtained was analyzed with the Analysis of Variant (ANOVA), followed by multiple range test duncan. The results showed that the highest percentage of motility, viability and intact plasma membrane derived from green tea extract 0,15% on skim milk and egg yolk diluent  for 1 day storage is 86.66b ± 2.58, 92.00b ± 1.78, and 74.16b ± 3.25. The lowest percentage of motility, viability and intact plasma membranes was obtained from the skim milk and egg yolk diluent for 5 days storage is 43.33a ± 6.05, 56.50a ± 3.08 and 28.33a ± 2.80. The conclusion of this study is the addition of 0.15% green tea extract in a skim milk and egg yolk diluent can maintain the quality of sperm for up to 5 days of storage at cold temperatures.

Keywords


Skim milk and egg yolk, green tea extract, sperm quality, Sapudi sheep

Full Text:

PDF

References


Agarwal, A., S. A. Prabakaran dan T. M. Said. 2005. Prevention of Oxydative Stress Minireview: Injury to Sperm. Journal of Andrology. 6 (26): 54-69. Aslam, H. A., Dasrul dan Rosmaidar. 2014. Pengaruh Penambahan Vitamin C dalam Pengencer Andromed terhadap Persentase Motilitas dan Membran Plasma Utuh Spermatozoa Sapi Aceh Setelah Pembekuan. Jurnal Medika Veterina. 8 (1): 20-26. Futino, D., M. Mendes., W. Matos., R. Mondadori and C. Lucci. 2010. Glycerol, Methyl-Formamide and Dimethyl-Formamide in Canine Semen Cryopre-servation. Reprod. Domest. Anim. 45: 214-220. Hardijanto, T., Sarjito., T. Hernawati dan T. W. Suprayogi. 2010. Buku Ajar Fisiologi dan Teknologi Reproduksi (IB). Airlangga University Press. Surabaya. Imanulkhan, 2006. Karakterisasi “Edibel Film” Beraktivitas dari Pati Ganyong (Canna edulis kerr) dan Ekstrak Teh Hijau (Camellia sinensis). Skripsi Mahasiswa Jurusan Teknologi Hasil Pertanian. Fakultas Teknologi Pertanian, Universitas Brawijaya. Malang. Kartasudjana, 2001. Teknik Inseminasi Buatan pada Ternak. Departemen Pendidikan Nasional. Lubis, T. M., Dasrul., C. L. Thasmi dan T. Akbar. 2013. Efektivitas Penambahan Vitamin C dalam Pengencer Susu Skim Kuning Telur terhadap Kualitas Spermatozoa Kambing Boer Setelah Penyimpanan Dingin. Jurnal S. Pertanian. 3(1): 347-361. Mumu, M. I. 2009. Viabilitas Semen Sapi Simmental yang Dibekukan Menggunakan Krioprotektan Gliserol. Journal Agroland. 16(2): 172-179. Naghma, K and H. Mukhtar. 2007. Tea Polyphenols for Health Promotions. Life Science. 81(7): 519-533. Risal, M dan Herdis. 2010. Peranan Antioksidan dalam Meningkatkan Kualitas Semen Beku. Wartazoa. 20(3). Said, S., M. Gunawan., E. M. Kaiin and B. Tappa. 2005. Daya Tahan Sperma Cair Sapi Simmental yang Disimpan dalam Straw dalam Temperatur 5°C. Artikel Ilmiah. Cibinang. Pusat Penelitian Bioteknologi. LIPI. Saleh, R. A dan A. Agarwal. 2002. Oxidative Stress and Male Infertility: from research bench to clinical practice. Journal Andrology. 23(6): 37-52. Sanoeka, D and M. Kurpisz. 2004. Reactive Oxygen Species and Sperm Cells. Reprod. Biol. Endoc. 2(12): 112-117. Sikka, S. C. 2004. Role of Oxydative Stress and Antioxidants in Andrology and Assisted Reproductive Technology. Journal Andrology. 25(1): 5-18. Sulistyo, J., Nurdiana dan H. Elizar. 2003. Pengembangan Kerja Sama Riset. Teknologi, Produksi dan Pemasaran Produk Hilir Teh. Prosiding “Simposium Teh Nasional 2003”. Pusat Penelitian Teh dan Kina Gambung. Bandung. Widjaya, N. 2011. Pengaruh Pemberian Susu Skim dengan Pengencer Tris Kuning Telur terhadap Daya Tahan Hidup Spematozoa Sapi pada Suhu Penyimpanan 5°C. Fakultas Pertanian Universitas Bandung Raya.


Refbacks

  • There are currently no refbacks.


Copyright (c) 2020 Wahyu Retno Swari, Emy Koestanti Sabdoningrum, Wurlina Wurlina, Suherni Susilowati, Rochmah Kurnijasanti, Erma Safitri

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

View My Stats

Creative Commons License
Ovozoa: Journal of Animal Reproduction by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

      

     

    

 

This journal is supported by