Intracervical Artificial Insemination using Frozen Semen of Boer buck on Pote Does at Different Times of Estrus
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Pote goats are kept by the rural community in Bangkalan Regency, Madura Island, Indonesia. Estrus symptoms in goats are usually unclear, causing low fertility. This study aimed to determine the intracervical artificial insemination at early and mid-estrus on the Pote does. The 24 the does were randomly divided into two groups and estrous synchronization with PGF2α of 200 µg intramuscularly, twice injections with 11 days intervals. The T1 groups of estrus the Pote does were inseminated at early estrus (0-12 hours). Meanwhile, T2 groups of estrus the Pote does were inseminated at mid-estrus (12-24 hours after signs of estrus appeared. The insemination conducted artificially using post-thawed Boer semen. The observed variables were non-return rate at day 21 post-insemination (NRR21), and pregnancy rate (PR) based on abdominal palpation diagnosis. The result showed that post-thawed Boer buck was qualified (sperm motility more than 40%) for artificial insemination. All of the Pote does were estrus after twice PGF2α intramuscular injection with 11 days intervals. The onset of estrus was 48-72 hours after the second injection PGF2α. The NRR21 and PR ranged from 66.66-100% and 37.50-66.66%, respectively. The NRR21 and PR of the T2 group were higher than those of the T1 group. It could be concluded that intracervical artificial insemination on Pote the does at mid-estrus were more effective, resulting in higher NRR21 and PR than those at early estrus.
Adisiswanto W. 2020. Bangkalan District in Numbers. Central Agency of Bangkalan Statistics. http://bappeda.bangkalankab.go.id/uploads/kabupaten_bangkalan_dalam_angka_2020.pdf
Al Yacoub, Gauly AN, Sohnrey M and Holtz B. 2011. Fixed-time deep uterine insemination in PGF2α-synchronized goats. Theriogenology.76:1730-1735.
Al'A'raaf QS, Sumaryadi MY, Prasetiyo Nugroho AP. 2020. Early Pregnancy DetectionIn Etawah Crossbreed Goat (Capra aegagrus hircus) Based On Non-Return Rate Method And Cubboni Reaction. ANGON: J Anim Sci Technol. 2: 147-155.
Arrebola FA, Torres-Martell R, González-Casquet O, Meza-Herrera CA, Pérez-Marín CC. 2022. Periovulatory Hormonal Profiles after Estrus Induction and Conception Rate by Fixed-Time AI in Payoya Goats during the Anestrous Season. Animals 12:2853.
Bazer FW, Thatcher WW. 2017. Chronicling the discovery of interferon tau. Reproduction. 154:F11-F20.
Budiyanto A, Savitri FK, Fibrianto YH. 2020. Study of Estrous Synchronization using PGF2α in Local Goats on Estrous Quality, Progesterone Concentration, and Pregnancy Rate. Jurnal Sain Veteriner 38: 272-279.
Carlos AF, Zimri CV, Pedro HB, Gilberto FF, Fabiola RB, Eduardo RF. 2019. Review: Function and regression of the corpus luteum during the estrous cycle. Abanico Vet. 9:1-21
Chanda T, Paul SR, Fakruzzaman M, Sultana M and Debnath GK. 2020. Productive and Reproductive Performance of Goat Breeds Under Semi- Intensive System in Bangladesh. IJSR. 26(2) : 253-255.
Dávila FS, González AS del B, Barragán HB. 2018. Reproduction in Goats. Goat Science.
Gervasi MG, Visconti PE. 2016. Chang's meaning of capacitation: A molecular perspective. Mol Reprod Dev. 83:860-874.
Goodling RC, Shook GE, Weigel KA, Zwald NR. 2005. The effect of synchronization on genetic parameters of reproductive traits in dairy cattle. J. Dairy Sci. 88: 2217-2225.
Hansen TR, Sinedino LDP, Spencer TE. 2017. Paracrine and endocrine actions of interferon tau (IFNT). Reproduction 154:F45-F59.
Holtz W, Sohnrey B, Gerland M and driancourt MA. 2008. Ovsynch synchronization andfixed- time insemination in goats. Theriogenology 69: 785-792.
INSA (Indonesian National Standard Agency); 2017. Frozen semen – Part 1: Bovine bull. Jakarta, Indonesia: Indonesian National Standardized Agency. https://bibit.ditjenpkh.pertanian.go.id/sites/default/files/SNI%204869-1-2017. [Accessed 30 January 2022].
Isabel OC, Mario FA, Felipe SC, Ana BC, Paula RC, Eduardo KNA and Felipe ZB. 2018. Accuracy of assessment of luteal morphology and luteal blood flow for prediction of early pregnancy in goats. Theriogenology 121: 104-111.
Islam MM, Kizaki K, Takahashi T, Khanom JS, Debnath S, Khandoker MAMY. 2014. Pregnancy diagnosis in Black Bengal goat by progesterone assay. Bangladesh J. Anim. Sci. 43: 180–184.
Karadaev M. 2015. Pregnancy diagnosis techniques in goats – a review. Bulg J Vet Med.18: 183–193.
Leigh OO, Lukman M, Chinyere AL, Ezenwanyi IA. 2018. Timed Artificial Insemination: Pregnancy Rates in Sokoto Gudali Cattle Treated with Prostaglandin F2 Alpha at a Private Dairy Farm in Nigeria. Anim Prod. 20:1-6.
Luo J, Wang W, Sun S. 2019. Research advances in reproduction for dairy goats. Asian-Australas J Anim Sci. 32:1284-1295.
Martemucci G and D'Alessandro, OG. 2011. Induction / Synchronization of oestrus and ovulation in dairy goats with different shortterm treatments and fixed time intrauterine of exocervical insemination system. Anim Reprod Sci. 126: 187-194.
Memon MA, Ott RS. 1980. Methods of pregnancy diagnosis in sheep and goats. Cornell Vet. 70:226-31.
Nogueira, D.M; Cavalieri, J; Gummow, B and Parker, A.J. 2015. Comparison of follicular dynamics and hormone profiles in Boer goats examined during the breeding and non breeding seasons in the tropics of Queensland, Auatralia. Small Rumin.Res. 125:93- 100.
Omontese BO. 2018. Estrus Synchronization and Artificial Insemination in Goats. Goat Science.
Pangestuningrum J, Madyawati SP, Eliyani H, Damayanti R, Rochmi SE. 2021.Etawa Goat Estrus Quality with Estrus Synchronization. JAVEST 2: 15-21
Rahmah UIL, Imanudin O, Permadi D. 2018. Analisis faktor-faktor yang berhubungan dengan tingkat keberhasilan Inseminasi Buatan pada kambing Kacang (Capra hircus). Jurnal Ilmu Pertanian dan Peternakan. 6: 180-189.
Raymundo RM, Santiago ZG, Gerardo AR, Fernando AR, Leticia GA, Viridiana CV. 2021. Effect of a short-term prostaglandin protocol upon synchronization and reproductive outcomes in cyclic goats. Abanico Veterinario 11:1-10.
Riaz U, Hassan M, Husnain A, Naveed MI, Singh J, Ahmad N. 2018. Effect of timing of artificial insemination in relation to onset of standing estrus on pregnancy per AI in Nili-Ravi buffalo. Anim Reprod. 15:1231-1235.
Salim MA, Ihsan MN, Isnaini N, Susilawati T. 2020. Kidding rate of artificial insemination with Boer goat liquid semen during chilled preservation using coconut water-based diluent. Jurnal Ilmu-Ilmu Peternakan 30: 184 – 189.
Salleh SM, Basri AMH, Yaakub H. 2021. Study of sexual behaviours with different types of estrus synchronization protocols in Boer goats. Anim Reprod. 18:e20200038.
Simoes J. 2016. Recent advances on syncronizhation of ovulation of goats, out of season, for a more sustainable production. Asian Pac J Reprod. 4: 157-165.
Susilowati S, Sardjito T, Mustofa I, Widodo S, Kurnijasanti R. 2021. Effect of green tea extract in extender of Simmental bull semen on pregnancy rate of recipients. Anim Biosci. 34: 198- 204..
Susilowati S, Triana IN, Sardjito T, Suprayogi TW, Wurlina W, Mustofa I. 2020. Effect of Simmental bull seminal plasma protein in egg yolk-citrate extender on Kacang buck semen fertility. Cryobiology 97:20-27.
Triagil AL, Ismudiono I, Setiawan B, Lestari TD, Samik A, Utomo B. 2020. Relationship between the value of the estrous detector measurement result and serum progesterone level in Etawa crossbred goats after estrous synchronization. Ovozoa 9: 82-85.
Yanagimachi R. 2022. Mysteries and unsolved problems of mammalian fertilization and related topics. Biol Reprod. 106:644-675.
Yotov AS, Desislava V, Velislavova and Lora DR. 2016. Pregnancy rate in Bulgarian White goats with natural and synchronized estrus after artificial insemination by frozen semen during breeding season. Asian Pac J Reprod. 5(2) : 144-147.
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