Effects of Honey Goat Milk Kefir on Pancreatic Histopathology and Renal Function in Diabetic Rats
Efek Pemberian Kefir Susu Kambing Madu pada Gambaran Histopatologi Pankreas dan Fungsi Ginjal pada Tikus Diabetes
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Background: The prevalence of diabetes is rising. Chronic metabolic disease type 2 diabetes may damage renal function and harm the pancreas. One of the initiatives to prevent and manage type 2 diabetes through functional foods. The antidiabetic and antioxidant properties of goat milk kefir with honey are thought to help heal kidney and pancreatic damage.
Objectives: Evaluating the effects of feading fermented goat milk with honey on pancreatic histopathology and renal function in diabetic rats.
Methods: A true experimental study conducted on 42 male Sprague-Dawley rats aged 6-8 weeks were divided into Six groups: Healthy Rats (KS); DM Rats (KN); quercetin (K1); metformin (K2); Kefir (P1); Preventive (P2). Diabetic rats type 2 were conditioned by HFD and injecting streptozotocin. Oral kefir was given 1.8 ml/200g BW/day for 21 days to groups KK and KP. Renal function was analyzed with pre-post tests through measurement of serum levels urea and creatinine, while the histopathological features were evaluated at the end of the study. The Kruskal-Wallis and post hoc Mann-Whitney test are used to analyze the data.
Results: P1 rats had Creatinine level of 0.57 ± 0.07 mg/dL; BUN 27.56 ± 6.22 mg/dL; Beta Cell Count 104.2; Island Area of Langerhans 170.26. The levels of BUN (p-value=0.083), creatinine (p-value=0.016), and Langerhans island area (p-value=0.026). Creatinine of rat P1 were significantly different from KN (p-value<0.05).
Conclusions: Goat milk kefir with honey in type 2 diabetic rats show beneficial effects on serum BUN, creatinine, and histopathological features of the pancreas as treated with metformin.
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