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Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Submitted
January 12, 2019
Accepted
January 12, 2019
Published
November 1, 2016

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Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik
[Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Probiotics]

https://doi.org/10.20473/jipk.v8i2.11182
Wahju Tjahjaningsih
Manajemen Kesehatan Ikan dan Budidaya Perikanan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.
Endang Dewi Masithah
Manajemen Kesehatan Ikan dan Budidaya Perikanan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.
Heru Pramono
Manajemen Kesehatan Ikan dan Budidaya Perikanan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.
Pipin Suciati
Program Studi Akuakultur, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.

Corresponding Author(s) : Wahju Tjahjaningsih

wahju.tjahjaningsih@fpk.unair.ac.id

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Abstrak
Probiotik dapat didefinisikan sebagai mikroba hidup yang ditambahkan dalam jumlah tertentu yang mampu bertahan hidup dalam ekosistem saluran pencernaan. Enzim yang dihasilkan oleh mikroba yang diisolasi dari saluran pencernaan ikan dapat digunakan sebagai probiotik. Enzim proteolitik ekstraseluler secara alami diproduksi oleh mikroba untuk menghidrolisis polipeptida dalam media menjadi peptida dan asam amino. Bakteri asam laktat dapat menghasil enzim seperti protease, α-amilase, fitase, kitinase, lipase. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.) yang mempunyai aktivitas proteolitik. Penelitian ini menggunakan metode deskriptif berupa aktivitas enzimatis dan karakterisasi uji biokimia isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.). Jumlah kepiting bakau yang digunakan adalah 10 ekor. Hasil penelitian didapatkan tiga isolat bakteri asam laktat yang memiliki aktivitas proteolitik kuat, yaitu WK 28, WK 33, dan WK 53. Hasil uji biokimia isolat WK 28 termasuk ke dalam genus Pediococcus sp., isolat WK 33 termasuk ke dalam Lactobacillus sp., dan isolat WK 53 termasuk ke dalam genus Streptococcus sp. WK 28 (Pediococcus sp.) dan WK 33 (Lactobacillus sp.) mempunyai aktivitas proteolitik dan aktivitas lipolitik. Isolat WK 53 (Streptococcus sp.) mempunyai aktivitas enzimatis yaitu aktivitas proteolitik, amilolitik, dan lipolitik.

Abstract
Probiotics are defined as live microbes are added in a certain amount that is able to survive in the digestive tract ecosystem. Enzymes produced by microbes isolated from the digestive tract of fish can be used as probiotics. Extracellular proteolytic enzymes naturally produced by the microbes to hydrolyze a polypeptide in a media into peptides and amino acids. Lactic acid bacteria can produce enzymes such as proteases, α-amylase, phytase, chitinase, lipase. This study aims to get the lactic acid bacteria isolates from the gastrointestinal tract of mangrove crab (Scylla spp.) That have proteolytic activity. This research uses descriptive method such as enzymatic activity and biochemical characterization of isolates of lactic acid bacteria from the digestive tract of mangrove crab (Scylla spp.). Amount of mud crab used is 10 fish. The result showed three isolates of lactic acid bacteria that have a strong proteolytic activity, namely WK 28 WK 33 and WK 53. The results of biochemical tests WK 28 isolates belonging to the genus Pediococcus sp., Isolate WK 33 belonging to the Lactobacillus sp., And WK 53 isolates belonging to the genus Streptococcus sp. WK 28 (Pediococcus sp.) And WK 33 (Lactobacillus sp.) Have proteolytic activity and lipolytic activity. Isolates WK 53 (Streptococcus sp.) Have enzymatic activity is proteolytic activity, amylolytic, and lipolytic.

Keywords

Mud crab lactic proteolytic amylolytic lipolytic
Tjahjaningsih, W., Masithah, E. D., Pramono, H., & Suciati, P. (2016). Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik <br><i>[Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Probiotics]<i>. Jurnal Ilmiah Perikanan Dan Kelautan, 8(2), 94–108. https://doi.org/10.20473/jipk.v8i2.11182
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