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Pengaruh Perbedaan Konsentrasi Gliserol dalam Susu Skim Kuning Telur untuk Proses Penyimpanan Sperma Beku terhadap Motilitas dan Viabilitas Spermatozoa Ikan Patin (Pangasius pangasius)
[Effect Of Different Glycerol Concentration In Skim Milk And Egg Yolk Diluter For Storage Process Of Frozen Semen On Sperm Motility And Viability Of Catfish (Pangasius pangasius)]
Corresponding Author(s) : Sudarno Sudarno
Jurnal Ilmiah Perikanan dan Kelautan,
Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
Needs of catfish seed always increase every years, especially for farming activities. Fulfillment demand of seed in large quntities and continuing are the main obstacle in the production process of catfish. This can be overcome by the process of cryopreservation. This process requires diluents and cryoprotectants to maintain the fertility of spermatozoa. Skim milk and egg yolks diluents has been used on fish because it was applied to carp and showing a good result of sperm motility and fertilization rate. Glycerol as cryoprotectant was reported effective to be used for European Catfish than the other kind cryoprotectant. This study aimed to determine the effect of different concentrations of glycerol in the diluent skim milk and egg yolk on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. This research method is the experiment with completely randomized design (CRD) as the experimental design. The treatment used is different glycerol concentrations, P1 (11%), P2 (13%), P3 (15%), and P4 (17%) and each of the treatment was repeated 5 times. The main parameters measured were motility (%) and viability (%) of spermatozoa. Supporting parameters include odor, color, pH, viscosity, sperm concentration and catfish (Pangasius pangasius). Analysis of the data using Analysis Of Variance (ANOVA) and to determine the best treatment performed Duncan's Multiple Range Test with 95% confidance interval. The results showed that the effect of the concentration of glycerol in the diluent skim milk and egg yolk was not significantly (P> 0.05) on the motility and viability of catfish (Pangasius pangasius) spermatozoa after freezing. Further testing needs to be done on the level of fertility of frozen sperm, as well as the growth rate of seeds produced from sperm frozen catfish (Pangasius pangasius) products
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- Adipu, Y., H. Sinjal, dan J. Watung. 2011. Ratio Pengenceran Sperma Terhadap Motilitas Spermatozoa, Fertilitas dan Daya Tetas Ikan Lele (Clarias sp.). Jurnal Perikanan dan Kelautan Tropis, 8 (1) : 48-55.
- Arifiantini, R. I., I. Supriatna, dan Samsurizal. 2007. Penentuan Waktu Equilibrasi pada Pembekuan Semen Kuda Menggunakan Bahan Pengencer Susu Skim. Animal Reproduction, 9 (3) : 145-152.
- Chew, P.C., Z.A. Rashid and R. Hassan. 2010. Application of Innovative Biotechnologies Regarding Aquaculture and Fisheries Sector in Malaysia: Cryopreservation Programme. Freshwater Fisheries Research Center. Malaysia. www.fao.org. 3 Juni 2013. 17 hal.
- Diwan, A.D., S. Ayyapan, K.K. Lal, and W.S. Lakra. 2010. Cryopreservation of Fish Gametes and Embryos. Indian Journal of Animal Sciences, 80 (4) : 109-124.
- Francis, T. C.A Devi, and M. Selvamagheswaran. 2013. Cryopreservaation of Carp Spermatozoa. Indian Journal of Science and Technology, 6 (5) : 4524-4530
- Fujaya, Y. 2002. Fisiologi ikan : Dasar Pengembangan Teknologi Perikanan. PT Rineka Cipta. Jakarta Gazali, M dan S. N. Tambing. 2002. Kriopreservasi Sel Spermatozoa. Hayati, 9 (1) : 27-32.
- Hardijanto, S. Susilowati, T. Hernawati, T. Sardjito, dan T. W. Suprayogi. 2010, Buku Ajar Inseminasi Buatan. Universitas Airlangga. Surabaya. Hal 40.
- Japet, N. 2011. Karakteristik Semen Ikan Ekonomis Budidaya : Mas (Cyprinus carpio), dan Patin (Pangasius hypothalamus). Skripsi. Institut Pertanian Bogor. Bogor. Hal : 20.
- Junior, M.Z., S. Handayani dan I. Supriatna. 2005. Kualitas Sperma Ikan Batak (Tor soro) Hasil Kriopreservasi Semen Menggunakan Dimetilsulfoksida (DMSO) dan Gliserol 5, 10 dan 15%. Jurnal Akuakultur Indonesia, 4 (2) : 145-151.
- Kementerian Kelautan dan Perikanan. 2010. Laporan Akhir Kegiatan Penelitian : Teknologi Pembenihan Ikan Patin (Pangasius sp.) yang Dipelihara Secara Outdoor di Kolam yang Dipupuk. Badan Penelitian dan Pengembangan Kelautan dan Perikanan.
- Sukamandi. Mar'ati, K. 2007. Pengaruh Dosis dan Lama Penyimpanan Pengencer Susu Skim Kuning Telur Terhadap Kualitas Semen Ikan Mas (Cyprinus carpio L.). Skripsi. Jurusan Biologi Fakultas Sains dan Teknologi. Universitas Islam Negeri Malang.
- Muchlisin, Z.A., R. Hashim, and A.S.C. Chong. 2004. Preliminary Study on The Cryopreservation of Tropical Bagrid Catfish (Mystus nemurus) Spermatozoa; The Effect of Extender and Cryoprotectant on The Motility After Short-Term Storage. Theriogenology, 62 (2004) : 25-34.
- Saparinto, C. 2010. Usaha Ikan Konsumsi di Lahan 100 m2. Penebar Swadaya. Jakarta. Setyono, B. 2009. Pengaruh Perbedaan Konsentrasi Bahan pada Pengencer Sperma Ikan "Skim Kuning Telur” terhadap Laju Fertilisasi, Laju Penetasan dan Sintasan Ikan Mas (Cyprinus carpio L). GAMMA, V (1) : 01-12.
- Slembrouck, J., O. Komarudin, Maskur. dan M. Legendre. 2005. Petunjuk Teknis Pembenihan Ikan Patin Indonesia, Pangasius djambal. Terjemahan: Subandi, A dan Khan, Z. IRD dan Pusat Riset Perikanan Budidaya, Badan Riset Kelautan dan Perikanan. Jakarta.
- Sulmartiwi, L., E. Ainurrohmah, dan A. S. Mubarak. 2011. Pengaruh Konsentrasi Air Kelapa Muda dan Madu dalam NaCl Fisiologis Terhadap Motilitas dan Lama Hidup Spermatozoa Ikan Patin (Pangasius pangasius). Jurnal Ilmiah Perikanan dan Kelautan, 3 (1) : 67-71.
- Susilowati, S. Hardijanto, T. W. Suprayogi, T. Sardjito, dan T. Hernawati. 2010. Penuntun Praktikum Inseminasi Buatan. Airlangga University Press. Surabaya.
- Sutrisna. 2002. Keragaman Kualitas Sperma Ikan Patin (Pangasius hypopthalmus Sauvage) pada Bulan Oktober 2000Februari 2001. Skripsi. Jurusan Budidaya Perairan. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor.
- Uddowla, M.H., U. Salma, M.M. Rahman, M.S. Hossain and M.F.A. Mollah. 2011. Effect of Diluents, Dilution Ratios and Cryoprotectants for The Cryopresevation of Thai Sharpunti (Barbodes gonionotus) spermatozoa. International Journal of Natural Sciences, 1 (2) : 44-51.
- Vishwanath, R. and P. Shannon. 2000. Storage of Bovine Semen in Liquid Frozen State. Animal Reproduction Science, 62 : 23-53.
- Widjaya, N. 2011. Pengaruh Pemberian Susu Skim dengan Pengencer Tris Kuning Telur terhadap Daya Tahan Hidup Spermatozoa Sapi pada Suhu Penyimpanan 5ºC. Sains Peternakan, 9 (2) : 72-76.
- Yavas, I., and Y. Bozkurt. 2011. Effect of Different Thawing Rates on Motility and Fertilizing Capacity of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm. Biotechnol & Biotechnol. 25 (1) : 2254-2257
References
Adipu, Y., H. Sinjal, dan J. Watung. 2011. Ratio Pengenceran Sperma Terhadap Motilitas Spermatozoa, Fertilitas dan Daya Tetas Ikan Lele (Clarias sp.). Jurnal Perikanan dan Kelautan Tropis, 8 (1) : 48-55.
Arifiantini, R. I., I. Supriatna, dan Samsurizal. 2007. Penentuan Waktu Equilibrasi pada Pembekuan Semen Kuda Menggunakan Bahan Pengencer Susu Skim. Animal Reproduction, 9 (3) : 145-152.
Chew, P.C., Z.A. Rashid and R. Hassan. 2010. Application of Innovative Biotechnologies Regarding Aquaculture and Fisheries Sector in Malaysia: Cryopreservation Programme. Freshwater Fisheries Research Center. Malaysia. www.fao.org. 3 Juni 2013. 17 hal.
Diwan, A.D., S. Ayyapan, K.K. Lal, and W.S. Lakra. 2010. Cryopreservation of Fish Gametes and Embryos. Indian Journal of Animal Sciences, 80 (4) : 109-124.
Francis, T. C.A Devi, and M. Selvamagheswaran. 2013. Cryopreservaation of Carp Spermatozoa. Indian Journal of Science and Technology, 6 (5) : 4524-4530
Fujaya, Y. 2002. Fisiologi ikan : Dasar Pengembangan Teknologi Perikanan. PT Rineka Cipta. Jakarta Gazali, M dan S. N. Tambing. 2002. Kriopreservasi Sel Spermatozoa. Hayati, 9 (1) : 27-32.
Hardijanto, S. Susilowati, T. Hernawati, T. Sardjito, dan T. W. Suprayogi. 2010, Buku Ajar Inseminasi Buatan. Universitas Airlangga. Surabaya. Hal 40.
Japet, N. 2011. Karakteristik Semen Ikan Ekonomis Budidaya : Mas (Cyprinus carpio), dan Patin (Pangasius hypothalamus). Skripsi. Institut Pertanian Bogor. Bogor. Hal : 20.
Junior, M.Z., S. Handayani dan I. Supriatna. 2005. Kualitas Sperma Ikan Batak (Tor soro) Hasil Kriopreservasi Semen Menggunakan Dimetilsulfoksida (DMSO) dan Gliserol 5, 10 dan 15%. Jurnal Akuakultur Indonesia, 4 (2) : 145-151.
Kementerian Kelautan dan Perikanan. 2010. Laporan Akhir Kegiatan Penelitian : Teknologi Pembenihan Ikan Patin (Pangasius sp.) yang Dipelihara Secara Outdoor di Kolam yang Dipupuk. Badan Penelitian dan Pengembangan Kelautan dan Perikanan.
Sukamandi. Mar'ati, K. 2007. Pengaruh Dosis dan Lama Penyimpanan Pengencer Susu Skim Kuning Telur Terhadap Kualitas Semen Ikan Mas (Cyprinus carpio L.). Skripsi. Jurusan Biologi Fakultas Sains dan Teknologi. Universitas Islam Negeri Malang.
Muchlisin, Z.A., R. Hashim, and A.S.C. Chong. 2004. Preliminary Study on The Cryopreservation of Tropical Bagrid Catfish (Mystus nemurus) Spermatozoa; The Effect of Extender and Cryoprotectant on The Motility After Short-Term Storage. Theriogenology, 62 (2004) : 25-34.
Saparinto, C. 2010. Usaha Ikan Konsumsi di Lahan 100 m2. Penebar Swadaya. Jakarta. Setyono, B. 2009. Pengaruh Perbedaan Konsentrasi Bahan pada Pengencer Sperma Ikan "Skim Kuning Telur” terhadap Laju Fertilisasi, Laju Penetasan dan Sintasan Ikan Mas (Cyprinus carpio L). GAMMA, V (1) : 01-12.
Slembrouck, J., O. Komarudin, Maskur. dan M. Legendre. 2005. Petunjuk Teknis Pembenihan Ikan Patin Indonesia, Pangasius djambal. Terjemahan: Subandi, A dan Khan, Z. IRD dan Pusat Riset Perikanan Budidaya, Badan Riset Kelautan dan Perikanan. Jakarta.
Sulmartiwi, L., E. Ainurrohmah, dan A. S. Mubarak. 2011. Pengaruh Konsentrasi Air Kelapa Muda dan Madu dalam NaCl Fisiologis Terhadap Motilitas dan Lama Hidup Spermatozoa Ikan Patin (Pangasius pangasius). Jurnal Ilmiah Perikanan dan Kelautan, 3 (1) : 67-71.
Susilowati, S. Hardijanto, T. W. Suprayogi, T. Sardjito, dan T. Hernawati. 2010. Penuntun Praktikum Inseminasi Buatan. Airlangga University Press. Surabaya.
Sutrisna. 2002. Keragaman Kualitas Sperma Ikan Patin (Pangasius hypopthalmus Sauvage) pada Bulan Oktober 2000Februari 2001. Skripsi. Jurusan Budidaya Perairan. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor.
Uddowla, M.H., U. Salma, M.M. Rahman, M.S. Hossain and M.F.A. Mollah. 2011. Effect of Diluents, Dilution Ratios and Cryoprotectants for The Cryopresevation of Thai Sharpunti (Barbodes gonionotus) spermatozoa. International Journal of Natural Sciences, 1 (2) : 44-51.
Vishwanath, R. and P. Shannon. 2000. Storage of Bovine Semen in Liquid Frozen State. Animal Reproduction Science, 62 : 23-53.
Widjaya, N. 2011. Pengaruh Pemberian Susu Skim dengan Pengencer Tris Kuning Telur terhadap Daya Tahan Hidup Spermatozoa Sapi pada Suhu Penyimpanan 5ºC. Sains Peternakan, 9 (2) : 72-76.
Yavas, I., and Y. Bozkurt. 2011. Effect of Different Thawing Rates on Motility and Fertilizing Capacity of Cryopreserved Grass Carp (Ctenopharyngodon idella) Sperm. Biotechnol & Biotechnol. 25 (1) : 2254-2257