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Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Aquaculture Fisheries Biotechnology

Accelerated of Sex Reversal use 17α-methyltestosterone Induced Female, Orange-Spotted Grouper Epinephelus coioides

https://doi.org/10.20473/jipk.v15i2.40624
Odang Carman
Departement of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java, 16680. Indonesia
https://orcid.org/0000-0001-9433-1318
Andri Iskandar
Study Program of Technology and Management of Applied Aquaculture Hatchery, College of Vocational Studies, IPB University, Bogor, West Java, 16680. Indonesia
https://orcid.org/0000-0002-7653-1743
Ching-Fong Chang
Department of Aquaculture, National Taiwan Ocean University, Keelung. Republic of China
Guan-Chung Wu
Center of Excellence for Marine Bioenvironmental and Biotechnology, National Taiwan Ocean University, Keelung. Republic of China
Muslim Muslim
Study Program of Aquaculture, Faculty of Agriculture, Sriwijaya University, Indralaya. Indonesia
https://orcid.org/0000-0001-5785-6369
Dian Eka Ramadhani
Study Program of Technology and Management of Applied Aquaculture Hatchery, College of Vocational Studies, IPB University, Bogor, West Java, 16680. Indonesia
https://orcid.org/0000-0001-9534-5041

Corresponding Author(s) : Odang Carman

odangca@apps.ipb.ac.id

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Highlight Research



  1. Sex reversal for orange-spotted grouper Epinephelus coioides

  2. The application of 17α-methyltestosterone induce sex change

  3. The stability of sex change need more investigation


Abstract


The occurance of hermaphrodites in grouper fish causes a scarcity of male parents, so an alternative is needed to accelerate sexchange to male at a young age. The present study was expected to scrutinize the mechanisms of sex-change in fish in the early change process, and whether the testis converted from immature ovary using 17α-methyltestosterone (MT) would recover after the termination of MT treatment. MT-induced sex-change and 5-aza-2'-deoxycytidine (5-Aza) were connected as DNA methylation inhibitors to comprehend the alternation of gonadal soma cells. The orange-spotted groupers were used  at the developmental ages and fed a diet containing MT at 50 mg/kg for three months and then a normal diet for a month. In the first week and second week fish injected with 5-Aza intraperitoneally during the MT-oral administration. Most of the fishes in the control group had immature ovaries, but all the females fed with MT, had immature spermatogenesis. However, one month after the withdrawal of MT treatment, the sex of the fish returned to female-like even though the fish have undergone MT-induced masculinization. This outcome demonstrates precocious sex-change from under yearling, orange-spotted grouper utilizing oral MT treatment is impermanent. All the females of 5-aza treatments showed no spermatogenic cells.  In this study, lower growth rates were demonstrated by the MT-treated groups. The impact of this metabolic change was clear after the end of the hormone oral administration since the decreased growth of the groups treated for three months.

Keywords

Aromatase InhibitorMethyltestosteroneOrange-Spotted GrouperSex-Change
Carman, O., Iskandar, A., Chang, C.-F., Wu, G.-C. ., Muslim, M. ., & Ramadhani, D. E. . (2023). Accelerated of Sex Reversal use 17α-methyltestosterone Induced Female, Orange-Spotted Grouper <I>Epinephelus coioides</I>. Jurnal Ilmiah Perikanan Dan Kelautan, 15(2), 264–277. https://doi.org/10.20473/jipk.v15i2.40624
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Author Biographies

Odang Carman, Departement of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java, 16680. Indonesia

Departement of Aquaculture, Faculty of Fisheries and Marine Science, IPB University, Bogor, West Java, 16680. Indonesia

Andri Iskandar, Study Program of Technology and Management of Applied Aquaculture Hatchery, College of Vocational Studies, IPB University, Bogor, West Java, 16680. Indonesia

Study Program of Technology and Management of Applied Aquaculture Hatchery, College of Vocational Studies, IPB University, Bogor, West Java, 16680. Indonesia

Ching-Fong Chang, Department of Aquaculture, National Taiwan Ocean University, Keelung. Republic of China

Department of Aquaculture, National Taiwan Ocean University, Keelung. Republic of China

Guan-Chung Wu, Center of Excellence for Marine Bioenvironmental and Biotechnology, National Taiwan Ocean University, Keelung. Republic of China

Center of Excellence for Marine Bioenvironmental and Biotechnology, National Taiwan Ocean University, Keelung. Republic of China

Muslim Muslim, Study Program of Aquaculture, Faculty of Agriculture, Sriwijaya University, Indralaya. Indonesia

Study Program of Aquaculture, Faculty of Agriculture, Sriwijaya University, Indralaya. Indonesia

Dian Eka Ramadhani, Study Program of Technology and Management of Applied Aquaculture Hatchery, College of Vocational Studies, IPB University, Bogor, West Java, 16680. Indonesia

Study Program of Technology and Management of Applied Aquaculture Hatchery, College of Vocational Studies, IPB University, Bogor, West Java, 16680. Indonesia

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