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2025: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2025)

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Fisheries Product Technology

Reduction of Raoultella ornithinolytica TN5 Biofilm using Hot Water and Nanochitosan

Khofifah Fajar Fitriani
Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
https://orcid.org/0009-0000-9216-6195
Prihati Sih Nugraheni
Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
https://orcid.org/0000-0002-6341-8397
Masagus Muhammad Prima Putra
Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
https://orcid.org/0000-0002-1866-4396
Indun Dewi Puspita
Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
https://orcid.org/0000-0003-3637-6870

Corresponding Author(s) : Indun Dewi Puspita

indun_dp@ugm.ac.id

Jurnal Ilmiah Perikanan dan Kelautan, 2025: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2025)

Abstract

Graphical Abstract



 


Highlight Research



  1. The biofilm formation of Raoultella ornithinolytica on a stainless steel surface was analyzed

  2. Longer duration of hot water immersion leading to a higher reduction of Raoultella ornithinolytica biofilm on stainless steel surface.

  3. Longer duration of nanochitosan exposure leading to a higher reduction of Raoultella ornithinolytica biofilm on stainless steel surface

  4. The combination treatment of hot water immersion and nanochitosan shows higher efficacy to reduce Raoultella ornithinolytica biofilm on stainless steel surface compare to sodium hypochlorite treatment.


 


 


Abstract


The equipment surfaces in food processing industries have the potential to contaminate products. Bacteria on a surface are able to form a biofilm. This study aimed to determine the effect of a combination treatment using hot water immersion and nanochitosan on the reduction of R. ornithinolytica’s biofilm on stainless steel surfaces. R. ornithinolytica was applied to a stainless steel surface, incubated at 30oC for 48 hours, and tested for its reduction using hot water immersion treatment with different times. The best result from this treatment was when it was used in combination. The viability of cells was determined using a swab and the total plate count method. A scanning electron microscope was used for qualitative observations of biofilm formed on stainless steel before and after sanitation. The result showed that 10 minutes of hot water immersion resulted in significant R. ornithinolytica biofilm reduction compared to 5 minutes of treatment (p<0.05). Furthermore, the combination treatment of 10 minutes of hot water with 15 minutes of nanochitosan (0.1%) immersion showed the highest percent reduction of R. ornithinolytica biofilm (p<0.05). The ability of the combination treatment to eliminate R. ornithinolytica biofilms is equivalent to or even better than sodium hypochlorite treatment.

Keywords

Hot water immersionNanochitosanBiofilmSodium hypochlorite
Fitriani, K. F. ., Nugraheni , P. S. ., Putra, M. M. P. ., & Puspita, I. D. (2025). Reduction of Raoultella ornithinolytica TN5 Biofilm using Hot Water and Nanochitosan. Jurnal Ilmiah Perikanan Dan Kelautan. Retrieved from https://e-journal.unair.ac.id/JIPK/article/view/74564
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Author Biographies

Khofifah Fajar Fitriani, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia

Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia

Prihati Sih Nugraheni , Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia

Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia

Masagus Muhammad Prima Putra, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia

Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia

Indun Dewi Puspita, Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia

Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia

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