Isolasi dan Identifikasi Vibrio sp. pada Produk Seafood Tradisional Area Timur Kota Surabaya
[Isolation and Identification of Vibrio sp. from Traditional Seafood Products of Eastern Surabaya City Area]

Heru Pramono, Hafizh M. Noor, Siti Sahatul Fatimah, Nur Aida Harahap, Alief Ayu Selia

= http://dx.doi.org/10.20473/jipk.v7i1.11223
Abstract views = 573 times | downloads = 450 times

Abstract


Abstrak
Vibrio sp. adalah patogen oportunis yang terdapat pada ekosistem muara dan lingkungan laut. Vibrio sp. Dapat menyebabkan gastroenteritis dan septikemia sedangkan V. alginolyticus memiliki peran penting dalam pembusukan produk seafood. Keberadaan Vibrio sp. dalam produk olahan seafood perlu diperhatikan karena salah satu agen kausatif penyebab food-borne illness. Tujuan penelitian ini adalah untuk mengisolasi dan mengidentifikasi Vibrio spp. Pada produk olahan seafood tradisional daerah Surabaya Timur. Total 24 sampel telah dibeli dari pasar ikan tradisional di seluruh wilayah Surabaya Timur, yang diolah dengan menggunakan sepuluh teknik yang berbeda. Dua belas isolat dengan ukuran koloni yang berbeda dan warna diisolasi dan didasarkan pada identifikasi sebelumnya, terdapat sembilan Vibrio sp. Hasil uji biokimia menunjukkan bahwa terdapat delapan isolat Vibrio alginolyticus dan satu V. parahaemolyticus. Keberadaan Vibrio sp. dalam produk olahan seafood tradisional harus menjadi perhatian sebelum karena produk tersebut siap untuk makan dan dapat sumber wabah penyakit yang disebabkan oleh makanan.


Keywords


Vibrio sp., produk olahan seafood tradisional, food-borne disease, Surabaya

Full Text:

PDF

References


Alam, M.J., Tomochika, K.I., Miyoshi, S.I., Shinoda, S., 2002. Environmental investigationof potentially pathogenic

Vibrio parahaemolyticus in the SetoInland Sea, Japan.FEMS. Microbiol. Lett. 208, 83–87

Austin, B., Austin, D.A., Blanch, A.R., Cerda, M., Grimont, F., Grimont, P.A.D., Jofre, J.,Koblavi, S., Larsen, J.L., Pedersen, K., Tiainen, T., Verdonck, L., Swings, J., 1997. Acomparison of methods for the typing of fishpathogenic Vibrio spp. Systematicand Applied Microbiology 20, 89–101

Chen, W., Y.Xie, J.Xu, Q. Wang, M.Gu, J.Yang, M.Zhou, D.Wang, C.Shi, X.Shi. 2012. Molecular typing of Vibrio parahaemolyticus isolated from the middle-east coastline of China. International Journal of Food Microbiology 153:402-412

DePaola, A., Ulaszek, J., Kaysner, C.A., Tenge, B.J., Nordstrom, J.L.,Well, J., Puhr, N., Gendel,S.M., 2003b. Molecular, serological, and virulence characteristics of Vibrio parahaemolyticus isolated from environmental, food, and clinical sources in NorthAmerica and Asia. Appl. Environ. Microbiol. 69, 3999– 4005

Farmer III, J.J., Janda, M., Brenner, F.W., Cameron, D.N., Birkhead, K.M.,2005. Genus I. Vibrio Pacini 1854, 411AL. In: Brenner, D.J., Krieg,N.R., Staley, J.T. (Eds.), Bergey’sManual of Systematic Bacteriology. The Proteobacteria. Part B. The Gammaproteobacteria, 2nd edn., vol.2. Springer, New York, pp. 494–546

Hara-Kudo, Y., S. Saito, K. Ohtsuka, S. Yamasaki, S. Yahito, T. Nishio, Y. Iwade, Y. Otomo, H. Konuma, H. Tanaka, H. Nakagawa, K. Sugiyama, Y. Sugita-Kamishi, S. Kumagai. 2012. Characteristics of a sharp decrease in Vibrio parahaemolyticus infection and seafood contamination in Japan. International Journal of Food Microbiology 157; 95-101

Horii, T., Morita, M., Muramatsu, H., Monji, A., Miyagishima, D., Kanno, T.,Maekawa,M., 2005. Antibiotic resistance in Aeromonas hydrophila and Vibrio alginolyticus from a wound infection: a case report. J. TraumaInjury Infect. Crit. Care 58, 196–200

Lesmana, M., D. Subekti, C.H. Simanjuntak, P. Tjaniadi, J.R. Campbell, B.A. Oyofo. 2001. Vibrio parahaemolyticus associated with cholera-like diarrhea among patients in North Jakarta, Indonesia. Diagnostic Microbiology and Infectious Disease 39: 71-75

Liu, X., Chen, Y., Wang, X., Ji, R., 2004. Foodborne disease outbreaks in China from 1992to 2001—national foodborne disease surveillance system. J. Hygiene Res. 33,725–727

Raghhunath, P., S. Acharya, A. Bhanumathi, I. Karunasagar. 2008. Detection and molecular characterization of Vibrio parahaemolyticus isolated from seafood harvested along the southest coast of India. Food Microbiology 25: 824-830

Robert-Pillot, A., S. Copin, C. Himbr, M. Gay, M.L. Wuilici. 2014. Occurrence of the three major Vibrio species pathogen for human in seafood products consumed in France using real-time PCR. International Journal of Food Microbiology 189: 75-81

Massad, G and J.D. Oliver. 1987. New selection and differential medium for Vibrio cholera and Vibrio vulnificus. Appl. Environ.Microbiol: 2262-2264

Miyamoto, Y., Kato, T., Obara, Y., Akiyama, S., Takizawa, K., Yamai, S., 1969. In vitrohemolytic characteristic of Vibrio parahaemolyticus: its close correlation with human pathogenicity. J. Bacteriol. 100, 1147–1149

Tuyet, D.T., Thiem, V.D., Von Seidlein, L., Chowdhury, A., Park, E., Canh, D.G., Chien, B.T.,Van Tung, T., Naficy, A., Rao,M.R., Ali,M., Lee, H., Sy, T.H., Nichibuchi,M., Clemens, J.,Trach, D.D., 2002. Clinical, epidemiological, and socioeconomic analysis of anoutbreak of Vibrio parahaemolyticus in Khanh Hoa Province, Vietnam. J. Infect. Dis.186, 1615–1620

Yang, Z.Q., X.A. Jiao, X.H. Zhou, G.X. Cao, W.M. Fang, R.X. Gu.2008. Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried, and salted seafood products in two coastal areas of eastern China. International Journal of Food Microbiology 125:279-285

Wong, H.C., Liu, S.H., Wang, T.K., Lee, C.L., Chiou, C.S., Liu, D.P., Nishibuchi, M., Lee, B.K.,2000. Characteristics of Vibrio parahaemolyticus O3:K6 from Asia. Appl. Environ.Microbiol. 66, 3981–3986


Refbacks

  • There are currently no refbacks.


Copyright (c) 2015 Jurnal Ilmiah Perikanan dan Kelautan

JIPK IS INDEXED BY :

                  

       

     

                      

           

 

             

          

     

                  

      

      

       

     

     

       

                         

  

View JIPK Stats

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

 

EDITORIAL  OFFICE

JURNAL ILMIAH PERIKANAN DAN KELAUTAN (JIPK) /SCIENTIFIC JOURNAL OF FISHERIES AND MARINE

FAKULTAS PERIKANAN DAN KELAUTAN, UNIVERSITAS AIRLANGGA
Kampus C UNAIR, Jl. Dharmahusada Permai No.330, Mulyorejo,
Kota Surabaya, Provinsi Jawa Timur. Indonesia. 60115
Telepon: (031) 5911451
Fax. (031) 5965741
Email : jipk@fpk.unair.ac.id

 

 

 This Journal is Supported by