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Isolasi dan Identifikasi Vibrio sp. pada Produk Seafood Tradisional Area Timur Kota Surabaya
[Isolation and Identification of Vibrio sp. from Traditional Seafood Products of Eastern Surabaya City Area]
Corresponding Author(s) : Heru Pramono
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 7 No. 1 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstrak
Vibrio sp. adalah patogen oportunis yang terdapat pada ekosistem muara dan lingkungan laut. Vibrio sp. Dapat menyebabkan gastroenteritis dan septikemia sedangkan V. alginolyticus memiliki peran penting dalam pembusukan produk seafood. Keberadaan Vibrio sp. dalam produk olahan seafood perlu diperhatikan karena salah satu agen kausatif penyebab food-borne illness. Tujuan penelitian ini adalah untuk mengisolasi dan mengidentifikasi Vibrio spp. Pada produk olahan seafood tradisional daerah Surabaya Timur. Total 24 sampel telah dibeli dari pasar ikan tradisional di seluruh wilayah Surabaya Timur, yang diolah dengan menggunakan sepuluh teknik yang berbeda. Dua belas isolat dengan ukuran koloni yang berbeda dan warna diisolasi dan didasarkan pada identifikasi sebelumnya, terdapat sembilan Vibrio sp. Hasil uji biokimia menunjukkan bahwa terdapat delapan isolat Vibrio alginolyticus dan satu V. parahaemolyticus. Keberadaan Vibrio sp. dalam produk olahan seafood tradisional harus menjadi perhatian sebelum karena produk tersebut siap untuk makan dan dapat sumber wabah penyakit yang disebabkan oleh makanan.
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- Alam, M.J., Tomochika, K.I., Miyoshi, S.I., Shinoda, S., 2002. Environmental investigationof potentially pathogenic
- Vibrio parahaemolyticus in the SetoInland Sea, Japan.FEMS. Microbiol. Lett. 208, 83–87
- Austin, B., Austin, D.A., Blanch, A.R., Cerda, M., Grimont, F., Grimont, P.A.D., Jofre, J.,Koblavi, S., Larsen, J.L., Pedersen, K., Tiainen, T., Verdonck, L., Swings, J., 1997. Acomparison of methods for the typing of ï¬shpathogenic Vibrio spp. Systematicand Applied Microbiology 20, 89–101
- Chen, W., Y.Xie, J.Xu, Q. Wang, M.Gu, J.Yang, M.Zhou, D.Wang, C.Shi, X.Shi. 2012. Molecular typing of Vibrio parahaemolyticus isolated from the middle-east coastline of China. International Journal of Food Microbiology 153:402-412
- DePaola, A., Ulaszek, J., Kaysner, C.A., Tenge, B.J., Nordstrom, J.L.,Well, J., Puhr, N., Gendel,S.M., 2003b. Molecular, serological, and virulence characteristics of Vibrio parahaemolyticus isolated from environmental, food, and clinical sources in NorthAmerica and Asia. Appl. Environ. Microbiol. 69, 3999– 4005
- Farmer III, J.J., Janda, M., Brenner, F.W., Cameron, D.N., Birkhead, K.M.,2005. Genus I. Vibrio Pacini 1854, 411AL. In: Brenner, D.J., Krieg,N.R., Staley, J.T. (Eds.), Bergey'sManual of Systematic Bacteriology. The Proteobacteria. Part B. The Gammaproteobacteria, 2nd edn., vol.2. Springer, New York, pp. 494–546
- Hara-Kudo, Y., S. Saito, K. Ohtsuka, S. Yamasaki, S. Yahito, T. Nishio, Y. Iwade, Y. Otomo, H. Konuma, H. Tanaka, H. Nakagawa, K. Sugiyama, Y. Sugita-Kamishi, S. Kumagai. 2012. Characteristics of a sharp decrease in Vibrio parahaemolyticus infection and seafood contamination in Japan. International Journal of Food Microbiology 157; 95-101
- Horii, T., Morita, M., Muramatsu, H., Monji, A., Miyagishima, D., Kanno, T.,Maekawa,M., 2005. Antibiotic resistance in Aeromonas hydrophila and Vibrio alginolyticus from a wound infection: a case report. J. TraumaInjury Infect. Crit. Care 58, 196–200
- Lesmana, M., D. Subekti, C.H. Simanjuntak, P. Tjaniadi, J.R. Campbell, B.A. Oyofo. 2001. Vibrio parahaemolyticus associated with cholera-like diarrhea among patients in North Jakarta, Indonesia. Diagnostic Microbiology and Infectious Disease 39: 71-75
- Liu, X., Chen, Y., Wang, X., Ji, R., 2004. Foodborne disease outbreaks in China from 1992to 2001”national foodborne disease surveillance system. J. Hygiene Res. 33,725–727
- Raghhunath, P., S. Acharya, A. Bhanumathi, I. Karunasagar. 2008. Detection and molecular characterization of Vibrio parahaemolyticus isolated from seafood harvested along the southest coast of India. Food Microbiology 25: 824-830
- Robert-Pillot, A., S. Copin, C. Himbr, M. Gay, M.L. Wuilici. 2014. Occurrence of the three major Vibrio species pathogen for human in seafood products consumed in France using real-time PCR. International Journal of Food Microbiology 189: 75-81
- Massad, G and J.D. Oliver. 1987. New selection and differential medium for Vibrio cholera and Vibrio vulnificus. Appl. Environ.Microbiol: 2262-2264
- Miyamoto, Y., Kato, T., Obara, Y., Akiyama, S., Takizawa, K., Yamai, S., 1969. In vitrohemolytic characteristic of Vibrio parahaemolyticus: its close correlation with human pathogenicity. J. Bacteriol. 100, 1147–1149
- Tuyet, D.T., Thiem, V.D., Von Seidlein, L., Chowdhury, A., Park, E., Canh, D.G., Chien, B.T.,Van Tung, T., Naï¬cy, A., Rao,M.R., Ali,M., Lee, H., Sy, T.H., Nichibuchi,M., Clemens, J.,Trach, D.D., 2002. Clinical, epidemiological, and socioeconomic analysis of anoutbreak of Vibrio parahaemolyticus in Khanh Hoa Province, Vietnam. J. Infect. Dis.186, 1615–1620
- Yang, Z.Q., X.A. Jiao, X.H. Zhou, G.X. Cao, W.M. Fang, R.X. Gu.2008. Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried, and salted seafood products in two coastal areas of eastern China. International Journal of Food Microbiology 125:279-285
- Wong, H.C., Liu, S.H., Wang, T.K., Lee, C.L., Chiou, C.S., Liu, D.P., Nishibuchi, M., Lee, B.K.,2000. Characteristics of Vibrio parahaemolyticus O3:K6 from Asia. Appl. Environ.Microbiol. 66, 3981–3986
References
Alam, M.J., Tomochika, K.I., Miyoshi, S.I., Shinoda, S., 2002. Environmental investigationof potentially pathogenic
Vibrio parahaemolyticus in the SetoInland Sea, Japan.FEMS. Microbiol. Lett. 208, 83–87
Austin, B., Austin, D.A., Blanch, A.R., Cerda, M., Grimont, F., Grimont, P.A.D., Jofre, J.,Koblavi, S., Larsen, J.L., Pedersen, K., Tiainen, T., Verdonck, L., Swings, J., 1997. Acomparison of methods for the typing of ï¬shpathogenic Vibrio spp. Systematicand Applied Microbiology 20, 89–101
Chen, W., Y.Xie, J.Xu, Q. Wang, M.Gu, J.Yang, M.Zhou, D.Wang, C.Shi, X.Shi. 2012. Molecular typing of Vibrio parahaemolyticus isolated from the middle-east coastline of China. International Journal of Food Microbiology 153:402-412
DePaola, A., Ulaszek, J., Kaysner, C.A., Tenge, B.J., Nordstrom, J.L.,Well, J., Puhr, N., Gendel,S.M., 2003b. Molecular, serological, and virulence characteristics of Vibrio parahaemolyticus isolated from environmental, food, and clinical sources in NorthAmerica and Asia. Appl. Environ. Microbiol. 69, 3999– 4005
Farmer III, J.J., Janda, M., Brenner, F.W., Cameron, D.N., Birkhead, K.M.,2005. Genus I. Vibrio Pacini 1854, 411AL. In: Brenner, D.J., Krieg,N.R., Staley, J.T. (Eds.), Bergey'sManual of Systematic Bacteriology. The Proteobacteria. Part B. The Gammaproteobacteria, 2nd edn., vol.2. Springer, New York, pp. 494–546
Hara-Kudo, Y., S. Saito, K. Ohtsuka, S. Yamasaki, S. Yahito, T. Nishio, Y. Iwade, Y. Otomo, H. Konuma, H. Tanaka, H. Nakagawa, K. Sugiyama, Y. Sugita-Kamishi, S. Kumagai. 2012. Characteristics of a sharp decrease in Vibrio parahaemolyticus infection and seafood contamination in Japan. International Journal of Food Microbiology 157; 95-101
Horii, T., Morita, M., Muramatsu, H., Monji, A., Miyagishima, D., Kanno, T.,Maekawa,M., 2005. Antibiotic resistance in Aeromonas hydrophila and Vibrio alginolyticus from a wound infection: a case report. J. TraumaInjury Infect. Crit. Care 58, 196–200
Lesmana, M., D. Subekti, C.H. Simanjuntak, P. Tjaniadi, J.R. Campbell, B.A. Oyofo. 2001. Vibrio parahaemolyticus associated with cholera-like diarrhea among patients in North Jakarta, Indonesia. Diagnostic Microbiology and Infectious Disease 39: 71-75
Liu, X., Chen, Y., Wang, X., Ji, R., 2004. Foodborne disease outbreaks in China from 1992to 2001”national foodborne disease surveillance system. J. Hygiene Res. 33,725–727
Raghhunath, P., S. Acharya, A. Bhanumathi, I. Karunasagar. 2008. Detection and molecular characterization of Vibrio parahaemolyticus isolated from seafood harvested along the southest coast of India. Food Microbiology 25: 824-830
Robert-Pillot, A., S. Copin, C. Himbr, M. Gay, M.L. Wuilici. 2014. Occurrence of the three major Vibrio species pathogen for human in seafood products consumed in France using real-time PCR. International Journal of Food Microbiology 189: 75-81
Massad, G and J.D. Oliver. 1987. New selection and differential medium for Vibrio cholera and Vibrio vulnificus. Appl. Environ.Microbiol: 2262-2264
Miyamoto, Y., Kato, T., Obara, Y., Akiyama, S., Takizawa, K., Yamai, S., 1969. In vitrohemolytic characteristic of Vibrio parahaemolyticus: its close correlation with human pathogenicity. J. Bacteriol. 100, 1147–1149
Tuyet, D.T., Thiem, V.D., Von Seidlein, L., Chowdhury, A., Park, E., Canh, D.G., Chien, B.T.,Van Tung, T., Naï¬cy, A., Rao,M.R., Ali,M., Lee, H., Sy, T.H., Nichibuchi,M., Clemens, J.,Trach, D.D., 2002. Clinical, epidemiological, and socioeconomic analysis of anoutbreak of Vibrio parahaemolyticus in Khanh Hoa Province, Vietnam. J. Infect. Dis.186, 1615–1620
Yang, Z.Q., X.A. Jiao, X.H. Zhou, G.X. Cao, W.M. Fang, R.X. Gu.2008. Isolation and molecular characterization of Vibrio parahaemolyticus from fresh, low-temperature preserved, dried, and salted seafood products in two coastal areas of eastern China. International Journal of Food Microbiology 125:279-285
Wong, H.C., Liu, S.H., Wang, T.K., Lee, C.L., Chiou, C.S., Liu, D.P., Nishibuchi, M., Lee, B.K.,2000. Characteristics of Vibrio parahaemolyticus O3:K6 from Asia. Appl. Environ.Microbiol. 66, 3981–3986