Issue

Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Date Log

Submitted
January 12, 2019
Accepted
January 12, 2019
Published
November 1, 2016

1. Copyright of the article is transferred to the journal, by the knowledge of the author, whilst the moral right of the publication belongs to the author.

2. The legal formal aspect of journal publication accessibility refers to Creative Commons Atribusi-Non Commercial-Share alike (CC BY-NC-SA),  (https://creativecommons.org/licenses/by-nc-sa/4.0/)

3. The articles published in the journal are open access and can be used for non-commercial purposes. Other than the aims mentioned above, the editorial board is not responsible for copyright violation

 

The manuscript authentic and copyright statement submission can be downloaded ON THIS FORM. 

Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik
[Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Probiotics]

https://doi.org/10.20473/jipk.v8i2.11182
Wahju Tjahjaningsih
Manajemen Kesehatan Ikan dan Budidaya Perikanan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.
Endang Dewi Masithah
Manajemen Kesehatan Ikan dan Budidaya Perikanan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.
Heru Pramono
Manajemen Kesehatan Ikan dan Budidaya Perikanan, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.
Pipin Suciati
Program Studi Akuakultur, Fakultas Perikanan dan Kelautan, Universitas Airlangga, Indonesia.

Corresponding Author(s) : Wahju Tjahjaningsih

wahju.tjahjaningsih@fpk.unair.ac.id

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 8 No. 2 (2016): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Abstrak
Probiotik dapat didefinisikan sebagai mikroba hidup yang ditambahkan dalam jumlah tertentu yang mampu bertahan hidup dalam ekosistem saluran pencernaan. Enzim yang dihasilkan oleh mikroba yang diisolasi dari saluran pencernaan ikan dapat digunakan sebagai probiotik. Enzim proteolitik ekstraseluler secara alami diproduksi oleh mikroba untuk menghidrolisis polipeptida dalam media menjadi peptida dan asam amino. Bakteri asam laktat dapat menghasil enzim seperti protease, α-amilase, fitase, kitinase, lipase. Penelitian ini bertujuan untuk mendapatkan isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.) yang mempunyai aktivitas proteolitik. Penelitian ini menggunakan metode deskriptif berupa aktivitas enzimatis dan karakterisasi uji biokimia isolat bakteri asam laktat dari saluran pencernaan kepiting bakau (Scylla spp.). Jumlah kepiting bakau yang digunakan adalah 10 ekor. Hasil penelitian didapatkan tiga isolat bakteri asam laktat yang memiliki aktivitas proteolitik kuat, yaitu WK 28, WK 33, dan WK 53. Hasil uji biokimia isolat WK 28 termasuk ke dalam genus Pediococcus sp., isolat WK 33 termasuk ke dalam Lactobacillus sp., dan isolat WK 53 termasuk ke dalam genus Streptococcus sp. WK 28 (Pediococcus sp.) dan WK 33 (Lactobacillus sp.) mempunyai aktivitas proteolitik dan aktivitas lipolitik. Isolat WK 53 (Streptococcus sp.) mempunyai aktivitas enzimatis yaitu aktivitas proteolitik, amilolitik, dan lipolitik.

Abstract
Probiotics are defined as live microbes are added in a certain amount that is able to survive in the digestive tract ecosystem. Enzymes produced by microbes isolated from the digestive tract of fish can be used as probiotics. Extracellular proteolytic enzymes naturally produced by the microbes to hydrolyze a polypeptide in a media into peptides and amino acids. Lactic acid bacteria can produce enzymes such as proteases, α-amylase, phytase, chitinase, lipase. This study aims to get the lactic acid bacteria isolates from the gastrointestinal tract of mangrove crab (Scylla spp.) That have proteolytic activity. This research uses descriptive method such as enzymatic activity and biochemical characterization of isolates of lactic acid bacteria from the digestive tract of mangrove crab (Scylla spp.). Amount of mud crab used is 10 fish. The result showed three isolates of lactic acid bacteria that have a strong proteolytic activity, namely WK 28 WK 33 and WK 53. The results of biochemical tests WK 28 isolates belonging to the genus Pediococcus sp., Isolate WK 33 belonging to the Lactobacillus sp., And WK 53 isolates belonging to the genus Streptococcus sp. WK 28 (Pediococcus sp.) And WK 33 (Lactobacillus sp.) Have proteolytic activity and lipolytic activity. Isolates WK 53 (Streptococcus sp.) Have enzymatic activity is proteolytic activity, amylolytic, and lipolytic.

Keywords

Mud crab lactic proteolytic amylolytic lipolytic
Tjahjaningsih, W., Masithah, E. D., Pramono, H., & Suciati, P. (2016). Aktivitas Enzimatis Isolat Bakteri Asam Laktat dari Saluran Pencernaan Kepiting Bakau (Scylla spp.) Sebagai Kandidat Probiotik <br><i>[Activity Enzymatic of Isolate Lactic Acid Bacteria from the Digestive Tract of Mud Crab (Scylla spp.) as a Candidate Probiotics]<i>. Jurnal Ilmiah Perikanan Dan Kelautan, 8(2), 94–108. https://doi.org/10.20473/jipk.v8i2.11182
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. Bairagi, A., K.S. Ghosh, S. K. Sen and A. K. Ray. 2002. Enzyme Producing Bacterial Flora Isolated from Fish Digestive Tracts. Aquaculture International, 10 : 109-121.
  2. Benson. 2001. Microbiological Applications. Laboratory Manual in General Microbiology. Eighth Edition. McGraw-Hill Science Company : New York. pp. 72-175.
  3. Chotimah, S.C. 2009. Peranan Streptococcus thermophillus dan Lactobacillus bulgaricus dalam Proses Pembuatan Yogurt. Jurnal Ilmu Peternakan. Vol. 4 (2) : 49.
  6. Fossi, B.T., F. Tavea, and R. Ndjouenkeu. 2005. Production and Partial Characterization Thermostable Amilase from Ascomycetes Yeast Strain Isolated from Starchy Soils. African Journal of Biotechnology, 4 (1) : 14-18.
  7. Frazier, W.C. and D.C. Westhoff. 1988. Food Microbiology. Fourth Edition. McGraw-Hill : USA. pp. 11-80.
  8. Frobisher, M. 1962. Fundamental of Microbiology. Seventh Edition. W.B. Saunders : USA. pp. 99-106, 172177, 248-251.
  9. Gismondo, M.R., L.Drago and A. Lombardi. 1999. Review of Probiotics Available to Modify Gastrointestinal Flora. Internasional Journal of Antimicrobial Agents, 12 : 287-292.
  10. Hardiningsih, R., R.N.R. Napitupulu dan T. Yulinery. 2006. Isolasi dan Uji Resistensi Beberapa Isolat Lactobacillus pada pH Rendah. Biodiversitas, 7 (1) : 15-17.
  11. Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley, and S.T. Wiliams. 1994. Bergey's Manual of Determinative Bacteriology. Williams and Wilkins : USA. pp. 530-566.
  12. Jusadi, D., E. Gandara dan I. Mokoginta. 2004. Pengaruh Penambahan Probiotik Bacillus sp. pada Pakan Komersil terhadap Konversi Pakan dan Pertumbuhan. Jurnal Akuakultur Indonesia, 3 (1) : 15-18.
  13. Khunajakr, N., A. Wongwicham, D. Moonmangmee and S. Tantipaiboonvut. 2008. Screening and Identification of Lactic Acid Bacteria Producing Antimicrobial Compounds From Pig Gastrointestinal Tracts. KMITL Sci. Tech. J., 8 (1) : 8-17.
  14. Lenkey, H.A.W. dan Soeharsono. 2010. Probiotik sebagai Makanan Fungsional. Probiotik. Widya Padjadjaran: Bandung. hal. 66-106.
  15. Melliawati, R., A.C. Djohan, and Yopi. 2015. Seleksi Bakteri Asam Laktat sebagai Penghasil Enzim Protease. Pros Sem Nas Masy Biodiv Indon, 1 (2) : 184-188.
  16. Nespolo, C.R. and A. Brandelli. 2010. Production of Bacteriocin-Like Substances by Lactic Acid Bacteria Isolated from Regional Ovine Cheese. Brazilian Journal of Microbiology, 41 : 1009-1018.
  17. Pollack, R.A., L. Findlay, W. Mondschein and R.R. Modesto. 2005. Laboratory Exercises in Microbiology. Second Edition. John Wiley and Sons, Inc : New Zaeland. pp. 44-45.
  18. Rohy, G.S., B.S. Rahardja dan Agustono. 2014. Jumlah Total Bakteri dalam Saluran Pencernaan Ikan Gurami (Osphronemus gouramy) dengan Pemberian Beberapa Pakan Komersial yang Berbeda. Jurnal Ilmiah Perikanan dan Kelautan, 6 (1) : 2124.
  19. Salminen, S., A. Ouwehand, Y. Benno and Y.K. Lee. 1999. Probiotics. Trends in Food Science and Technology, 10 : 107-110.
  20. Setyati, W.A. dan Subagiyo. 2012. Isolasi dan Seleksi Bakteri Penghasil Enzim Ekstraseluler (Proteolitik, Amilolitik, Lipolitik dan Selulolitik) yang Berasal dari Sedimen Kawasan Manggrove. Ilmu Kelautan, 17 (3) : 164-168.
  21. Sjofjan, O. and T. Ardyati. 2011. Extracellular Amylase Activity of Amylolytic Bacteria Isolated from Quail's (Coturnix japonica) Intestinal Tract in Corn Flour Medium. International Journal of Poultry Science, 10 (5) : 411-415.
  22. Sumarsih, S., B. Sulistiyanto, H.S. Adi dan C.S. Utama. 2010. Pengaruh Aras Starter Lactobacillus sp. terhadap Performa Mikrobiologi Silase Ikan Dilihat dari Total Bakteri, Bakteri Asam Laktat dan Fungi. Jurnal Kesehatan, 3 (1) : 43-50.
  23. Suryani, Y., Astuti, B. Oktavia dan S. Umniyati. 2010. Isolasi dan Karakterisasi Bakteri Asam Laktat dari Limbah Kotoran Ayam sebagai Agensi Probiotikdan Enzim Kolesterol Reduktase. Prosiding Seminar Nasional Biologi. Universitas Negeri Yogyakarta. hal. 138-147.
  24. Svetlitshnyl, V., F. Rainey, and J. Wiegel. 1996. Thermosyntropha Lipolytica Grn. Nov., sp. Nov., a Lipolytic, Anaerobic, Alkalitolerant, Thermophilic Bacterium Utilizing Short and Long Chain Fatty Acidin Syntrophic Coculture with a Methanogenic Archaeum. International Journal of Systematic Bacteriology, 46: 1131-1137.
  25. Telussa, I. 2013. Isolasi Bakteri Penghasil Enzim Lipase dari Coco ButterSubtitute dan Karakterisasi Lipasenya. Prosiding FMIPA Universitas Pattimura. 10 hal.
  26. Triyanto, A. Isnansetyo, I.D. Prijambada, J. Widada dan A. Tarmiawati. 2009. Isolasi, Karakterisasi dan Uji Infeksi Bakteri Proteolitik dari Lumpur Kawasan Hutan Bakau. Jurnal Perikanan (J. Fish. Sci), 11 (1): 13-18.
  27. Wilson, P. and Z. Remigio. 2012. Production and Characterisation of Protease Enzyme Produced by a Novel Moderate Thermophilic Bacterium (EP1001) Isolated from an Alkaline Hot Spring, Zimbabwe. African Journal of Microbiology Research, 6 (27) : 5542-5551.
  28. Yelnetty, A., H. Purnomo, Purwadi, A. Mirah. 2014. Biochemical Characteristics of Lactic Acid Bacteria with Proteolytic Activity and Capability as Starter Culture Isolated from Spontaneous Fermented Local Goat Milk. Journal of Natural Sciences Research, 4 (10) : 137-146.
  29. Yusmarini, R. Indrati, T. Utami dan Y. Marsono. 2009. Isolasi dan Identifikasi Bakteri Asam Laktat Proteolitik dari Susu Kedelai yang Terfermentasi Spontan. Jurnal Natur Indonesia, 12 (1) : 28-33.
Read More
Author biographies is not available.
Download this PDF file
PDF
Altmetric Badge
Trend MD
Statistic
Read Counter : 5419 Download : 31366

Downloads

Download data is not yet available.

Most read articles by the same author(s)

1 2 3 > >>