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The Ability of Spirulina sp. Microalgae as A Phytoremediation Agents in Liquid Waste of Handling Fish from Cemara Market, Medan
Corresponding Author(s) : Astrid Fauzia Dewinta
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 12 No. 2 (2020): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Highlight
- Microalgae Spirullina sp. can remediate BOD levels reach on 75.59% with the experiment was 30% liquid waste and 70% water.
- Microalgae Spirullina sp. can remediate COD levels reach on 79.19% with the experiment was 30% liquid waste and 70% water.
- Microalgae Spirullina sp. can remediate TSS levels reach on 83.54% with the experiment was 30% liquid waste and 70% water.
- Microalgae Spirullina sp. can remediate ammonia levels reach on 89.15% with the experiment was 30% liquid waste and 70% water.
Contamination material from fish market activities, namely fish handling liquid waste, is a problem facing the city of Medan today. Reduce the level of liquid waste pollutants can be done biologically by using microalgae organisms. One of them is like microalgae Spirulina sp. This study aims to determine the ability of Spirulina sp. for reducing the levels of pollutants in liquid waste of handling fish.There search method was use a Completely Randomized Design (CRD) with five treatments and three replications. Addition of 100 mL of inoculant Spirulina sp. with a density of 1x105 ind/mL of liquid waste mixed with fresh water with to reach a volume of 1000 mL. Each liquid waste concentration of 30%, 60%, 80%, 100%, and control (0%) added 1 mL Walne fertilizer. The results of initial measurements of liquid waste for parameters BOD, COD, TSS, ammonia, and phosphate, respectively are 26.50mg/L, 4400mg/L, 894mg/L, 1.10mg/L, and 16.7 mg/L. Based on the results, the best reduction in BOD level occured in the treatment of 30% (pA) of liquid waste is 17.64 mg/L with phytoremediation efficiency of 75.59%. The best reduction in COD levels occurred in the treatment of 30% (pA) of liquid waste is 1301.12 mg/L with phytoremediation efficiency of 79.19%. The best reduction in TSS levels occurred in the treatment of 60% (pB) of liquid waste is 411 mg/L with phytoremediation efficiency of 83.54%. The best reduction in ammonia levels was at 30% (pA) treatment, which was 0.38 mg/L with phytoremediation efficiency of 89.15%. While the best decrease in phosphate levels occurred in the control treatment (pK), which was 4.45 mg/L with phytoremediation efficiency of 97.35%.
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- Abdel-Raouf, N.,Al-Homaidan, A.A., &Ibraheem, I.B.M. (2012). Microalgae and Wastewater Treatment. Saudi Journal of Biological Sciences. 19 (3): 257–275.
- Abdel-Shafy, H. I., & Mansour, M. S. M. (2018). Phytoremediation for the Elimination of Metals, Pesticides, PAHs, and Other Pollutants from Wastewater and Soil. In: Kumar V., Kumar M., Prasad R. (eds) Phytobiont and Ecosystem Restitution. Springer, Singapore.
- Ahammad, S. Z., Graham, D. W., & Dolfing, J. (2013). Wastewater Treatment: Biological. In book: Encyclopedia of Environmental Management. Pp. 2645-2655. Taylor and Francis Publisher.
- Asmara, A. (2005). Hubungan Struktur Komunitas Plankton dengan Kondisi Fisika-Kimia Perairan Pulau Pramuka dan Pulau Panggang, Kepulauan Seribu. Institut Pertanian Bogor, Bogor.
- Astiani, F., I. Dewiyanti & S. Mellisa. (2016). Pengaruh Media Kultur yang Berbeda Terhadap Laju Pertumbuhan dan Biomassa Spirulina sp. Jurnal Ilmiah Mahasiswa Kelautan dan Perikanan Unsyiah. 1 (3): 441-447. ISSN. 2527-6395.
- Baharsyah, A. M. (2014). Pelepasan Fosfor dari Keramba Jaring Apung Ikan Mas (Cyprinus Carpio) di Waduk Cirata. Institut Pertanian Bogor, Bogor.
- Cahyanto, T., T. Sudjarwo, S. P. Larasati, & A. Fadillah. (2018). Fitoremediasi Air Limbah Pencelupan Batik parakannyasag Tasikmalaya Menggunakan Ki Apu(Pistia stratiotes L.). Scripta Biologica. 5 (2) :83–89.
- Dejsungkranont, M., Phoopat, N., & Sirisansaneeyakul, S. (2012). Optimization of the Biomass Production of Arthrospira (Spirulina) Using Taguchi Method. The Open Conference Proceedings Journal. 3:70-81.
- Delgadillo-Mirquez, L., Lopes, F.,Taidi B., & Pareau, D. (2016). Nitrogen and phosphate removal from wastewater with a mixed microalgae and bacteria culture. Biotechnology Reports. 11: 18-26.
- Effendi, H. (2003). Telaah Kualitas Air. Penerbit Kanisius. Yogyakarta.
- Ekantari, N., Y. Marsono, Y. Pranoto, & E. Harmayani. (2017). Pengaruh Media Budidaya Menggunakan Air Laut dan Air Tawar terhadap Sifat Kimia dan Fungsional Biomassa Kering Spirulina platensis. Agritech. 37(2):173-182.
- Elystia, S., S. R. Muria, & S. I. P. Pertiwi. (2019). Pemanfaatan Mikroalga Chlorella Sp. untuk Produksi Lipid dalam Media Limbah Cair Hotel dengan Variasi Rasio C:N dan Panjang Gelombang Cahaya. Jurnal Sains dan Teknologi Lingkungan, 11(1): 25-43.
- Fagiri, Y.M.A., Salleh, A., & El-Nagerabi, S. A. F. (2013). Impact of Physico-Chemical Parameters On The Physiological growth of Arthrospira (Spirulina platensis) Sexogenous Strain UTEXLB2340. African Journal of Biotechnology. 12 (35): 5458-5665.
- Hadiyanto & M. Christwardanaa. (2012). Aplikasi Fitoremediasi Limbah Jamu dan Pemanfaatannya untuk Produksi Protein. JurnalIlmuLingkungan, 10(1): 129-134.
- Hadiyanto,Christwardana, M., & Soetrisnanto, D. (2013). Phytoremediations of palm oil mill effluent (POME) using aquatic plants and microalgae for biomass production. Journal of Environmental Science and Technology. 6(2): 79–90.
- Handajani, H. (2006). Pemanfaatan Limbah Cair Tahu sebagai Pupuk Alternatif pada Kultur Mikroalga Spirullina sp. Jurnal Protein. 13(2).
- Ismaiel, M. M. S., El-Ayouty, Y. M., & Normorea, M. P. (2016). Role of pH on antioxidants production by Spirulina (Arthrospira) platensis. Brazilian Journal of Microbiology. 47(2): 298–304.
- Khalila, H. S., Fayed. W. M., Mansour, A. T., Srour, T. M., Omar, E. A., Darwish, S. I., & Nour, A. A. M. (2018). Dietary Supplementation of Spirulina, Arthrospira platensis, With Plant Protein Sources and their Effects on Growth, Feed Utilization and Histological Changes in Nile Tilapia, Oreochromis niloticus. Journal of Aquaculture Research & Development. 9(10):1-9.
- Loera-Quezada, M.M., Leyva-González, M.A., López-Arredondo, D., & Herrera-Estrella, L. (2015). Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae. Plant Science. 231: 124–130.
- Lutzu, G. A. (2011). Analysis of the growth of microalgae in batch and semi batch photobioreactors. [Disertasi]. Universita Degli Studi di Cagliari, Cagliari.
- Megalina, Y. (2016). Menganalisis Pencemaran Daerah Aliran Sungai (DAS) Akibat Limbah Domestik di kota Medan. Jurnal Ikatan Alumni Fisika Universitas Negeri Medan. 2 (2). ISSN : 2461-124759.
- Muliani, Ayuzar E., & Amri, M. C. (2018). Pengaruh Pemberian Pupuk Kascing (Bekas Cacing) yang di Fermentasi dengan Dosis yang Berbeda dalam Kultur Spirulina sp. Acta Aquatica: Aquatic Sciences Journal, (5)1: 30-35.
- Olguín, E.J., Galicia, S., Angulo-Guerrero, O., & Hernández, E. (2001). The Effect of Low Light Flux and Nitrogen Deficiency on the Chemical Composition of Spirulina sp. (Arthrospira) Grown on Digested Pig Waste. Bioresource Technology. 77(1): 19–24.
- Pacheco, M.M., Hoeltz, M., Moraes, M.S., & Schneider, R.C. (2015). Microalgae: Cultivation Techniques and Wastewater Phycoremediation. Journal of Environmental Science and Health. 50(6): 585–601.
- Pamungkas, M. T.O.A. (2016). Studi Pencemaran Limbah Cair dengan Parameter BOD5 Dan pH di Pasar Ikan Tradisional dan Pasar Modern di Kota Semarang. Jurnal Kesehatan Masyarakat. 4 (2): 166-175.
- Prasadi, O. (2018). Pertumbuhan dan Biomasa Spirulina sp. dalam Media Pupuk sebagai Bahan Pangan Fungsional. Jurnal Ilmu Perikanan Kelautan. (10) 2: 119-123.
- Pratama, E. (2017). Fitoremediasi Limbah Budidaya Pendederan Kerapu Bebek (Cromileptesaltivelis) Menggunakan Spirulina sp. Universitas Lampung, Bandar Lampung.
- Qurbani, N. P. (2015). Deteksi Kualitas Air Menggunakan Bentos di Sungai Way Sekampung, Metro Kibang Lampung Timur. Universitas Lampung, Bandar Lampung.
- Rajasekaran, C., Ajeesh, C. P. M., Balaji S., Shalini, M., Siva, B., Das, R., Fulzele, D. P., & Kalaivani, T. (2016). Effect of Modified Zarrouk's Medium on Growth of Different Spirulina Strains. Walailak Journal of Science & Technology. 13(1): 67-75.
- Rinawati, D., Hidayat, R. Suprianto, & P. S.Dewi. (2016). Penentuan Kandungan Zat Padat (Total Dissolve Solid dan Total Suspended Solid) di Perairan Teluk Lampung. Analit: Analytical and Environmental Chemistry.1(1).
- Sigiro, P.S., & Rokaya, E. (2016). Efektifitas Penyerapan Timbal (Pb) oleh Bunga Matahari (Helianthus annuus LINN) Menggunakan Penambahan Mikoriza dan Edta. UAJY. Universitas Dipenogoro, Semarang.
- Simamora, L. A., Sudarno, & T. Istirokhatun. (2017). Kultivasi Mikroalga Sebagai Metode Pengolahan dalam Menyisihkan Kadar COD dan Amonium pada Limbah Cair Tahu. Jurnal Teknik Lingkungan. 6(1):1-14.
- Siregar, A., D. Jubaedah, & M. Wijayanti. (2017). Penggunaan Hydrilla verticillata Sebagai Fitoremediator dalam Pemeliharaan Ikan Patin (Pangasius sp.). Jurnal Akuakultur Rawa Indonesia, 5(1) :70-82
- Sudjarwo T, N. Nisyawati, Rossiana, & W. Mangunwardoyo. (2014). The growth of water hyacinth (Eichhorniacrassipes (Mart.) Solms) and water lettuce (Pistia stratiotes L.) in domestic wastewater in wastewater treatment plant (WWTP) Bojongsoang, Bandung, Indonesia. Journal of Biodiversity and Environmental Sciences. 5(4):393–401.
- Uebel, L. S., Costa, J. A. V., Olson, A. C., & Morais, M. G. (2019). Industrial Plant For Production Of Spirulina sp. LEB 18. Brazilian Journal of Chemical Engineering. 36(1).
- Wahyuni, N., E. D. Masithah, W. Soemarjati, Suciyono, & M. F. Ulkhaq. (2018). Pola Pertumbuhan Mikroalga Spirulina sp. Skala Laboratorium yang Dikultur Menggunakan Wadah yang Berbeda. Majalah Ilmiah Bahari Jogja, 16(2): 89-97.
- Wijaya, H. K. (2009). Komunitas Perifiton dan Fitoplankton Serta Parameter Fisika Kimia Perairan Sebagai Penentu Kualitas Air di Bagian Hulu Sungai Cisadane, Jawa Barat. Institut Pertanian Bogor, Bogor.
References
Abdel-Raouf, N.,Al-Homaidan, A.A., &Ibraheem, I.B.M. (2012). Microalgae and Wastewater Treatment. Saudi Journal of Biological Sciences. 19 (3): 257–275.
Abdel-Shafy, H. I., & Mansour, M. S. M. (2018). Phytoremediation for the Elimination of Metals, Pesticides, PAHs, and Other Pollutants from Wastewater and Soil. In: Kumar V., Kumar M., Prasad R. (eds) Phytobiont and Ecosystem Restitution. Springer, Singapore.
Ahammad, S. Z., Graham, D. W., & Dolfing, J. (2013). Wastewater Treatment: Biological. In book: Encyclopedia of Environmental Management. Pp. 2645-2655. Taylor and Francis Publisher.
Asmara, A. (2005). Hubungan Struktur Komunitas Plankton dengan Kondisi Fisika-Kimia Perairan Pulau Pramuka dan Pulau Panggang, Kepulauan Seribu. Institut Pertanian Bogor, Bogor.
Astiani, F., I. Dewiyanti & S. Mellisa. (2016). Pengaruh Media Kultur yang Berbeda Terhadap Laju Pertumbuhan dan Biomassa Spirulina sp. Jurnal Ilmiah Mahasiswa Kelautan dan Perikanan Unsyiah. 1 (3): 441-447. ISSN. 2527-6395.
Baharsyah, A. M. (2014). Pelepasan Fosfor dari Keramba Jaring Apung Ikan Mas (Cyprinus Carpio) di Waduk Cirata. Institut Pertanian Bogor, Bogor.
Cahyanto, T., T. Sudjarwo, S. P. Larasati, & A. Fadillah. (2018). Fitoremediasi Air Limbah Pencelupan Batik parakannyasag Tasikmalaya Menggunakan Ki Apu(Pistia stratiotes L.). Scripta Biologica. 5 (2) :83–89.
Dejsungkranont, M., Phoopat, N., & Sirisansaneeyakul, S. (2012). Optimization of the Biomass Production of Arthrospira (Spirulina) Using Taguchi Method. The Open Conference Proceedings Journal. 3:70-81.
Delgadillo-Mirquez, L., Lopes, F.,Taidi B., & Pareau, D. (2016). Nitrogen and phosphate removal from wastewater with a mixed microalgae and bacteria culture. Biotechnology Reports. 11: 18-26.
Effendi, H. (2003). Telaah Kualitas Air. Penerbit Kanisius. Yogyakarta.
Ekantari, N., Y. Marsono, Y. Pranoto, & E. Harmayani. (2017). Pengaruh Media Budidaya Menggunakan Air Laut dan Air Tawar terhadap Sifat Kimia dan Fungsional Biomassa Kering Spirulina platensis. Agritech. 37(2):173-182.
Elystia, S., S. R. Muria, & S. I. P. Pertiwi. (2019). Pemanfaatan Mikroalga Chlorella Sp. untuk Produksi Lipid dalam Media Limbah Cair Hotel dengan Variasi Rasio C:N dan Panjang Gelombang Cahaya. Jurnal Sains dan Teknologi Lingkungan, 11(1): 25-43.
Fagiri, Y.M.A., Salleh, A., & El-Nagerabi, S. A. F. (2013). Impact of Physico-Chemical Parameters On The Physiological growth of Arthrospira (Spirulina platensis) Sexogenous Strain UTEXLB2340. African Journal of Biotechnology. 12 (35): 5458-5665.
Hadiyanto & M. Christwardanaa. (2012). Aplikasi Fitoremediasi Limbah Jamu dan Pemanfaatannya untuk Produksi Protein. JurnalIlmuLingkungan, 10(1): 129-134.
Hadiyanto,Christwardana, M., & Soetrisnanto, D. (2013). Phytoremediations of palm oil mill effluent (POME) using aquatic plants and microalgae for biomass production. Journal of Environmental Science and Technology. 6(2): 79–90.
Handajani, H. (2006). Pemanfaatan Limbah Cair Tahu sebagai Pupuk Alternatif pada Kultur Mikroalga Spirullina sp. Jurnal Protein. 13(2).
Ismaiel, M. M. S., El-Ayouty, Y. M., & Normorea, M. P. (2016). Role of pH on antioxidants production by Spirulina (Arthrospira) platensis. Brazilian Journal of Microbiology. 47(2): 298–304.
Khalila, H. S., Fayed. W. M., Mansour, A. T., Srour, T. M., Omar, E. A., Darwish, S. I., & Nour, A. A. M. (2018). Dietary Supplementation of Spirulina, Arthrospira platensis, With Plant Protein Sources and their Effects on Growth, Feed Utilization and Histological Changes in Nile Tilapia, Oreochromis niloticus. Journal of Aquaculture Research & Development. 9(10):1-9.
Loera-Quezada, M.M., Leyva-González, M.A., López-Arredondo, D., & Herrera-Estrella, L. (2015). Phosphite cannot be used as a phosphorus source but is non-toxic for microalgae. Plant Science. 231: 124–130.
Lutzu, G. A. (2011). Analysis of the growth of microalgae in batch and semi batch photobioreactors. [Disertasi]. Universita Degli Studi di Cagliari, Cagliari.
Megalina, Y. (2016). Menganalisis Pencemaran Daerah Aliran Sungai (DAS) Akibat Limbah Domestik di kota Medan. Jurnal Ikatan Alumni Fisika Universitas Negeri Medan. 2 (2). ISSN : 2461-124759.
Muliani, Ayuzar E., & Amri, M. C. (2018). Pengaruh Pemberian Pupuk Kascing (Bekas Cacing) yang di Fermentasi dengan Dosis yang Berbeda dalam Kultur Spirulina sp. Acta Aquatica: Aquatic Sciences Journal, (5)1: 30-35.
Olguín, E.J., Galicia, S., Angulo-Guerrero, O., & Hernández, E. (2001). The Effect of Low Light Flux and Nitrogen Deficiency on the Chemical Composition of Spirulina sp. (Arthrospira) Grown on Digested Pig Waste. Bioresource Technology. 77(1): 19–24.
Pacheco, M.M., Hoeltz, M., Moraes, M.S., & Schneider, R.C. (2015). Microalgae: Cultivation Techniques and Wastewater Phycoremediation. Journal of Environmental Science and Health. 50(6): 585–601.
Pamungkas, M. T.O.A. (2016). Studi Pencemaran Limbah Cair dengan Parameter BOD5 Dan pH di Pasar Ikan Tradisional dan Pasar Modern di Kota Semarang. Jurnal Kesehatan Masyarakat. 4 (2): 166-175.
Prasadi, O. (2018). Pertumbuhan dan Biomasa Spirulina sp. dalam Media Pupuk sebagai Bahan Pangan Fungsional. Jurnal Ilmu Perikanan Kelautan. (10) 2: 119-123.
Pratama, E. (2017). Fitoremediasi Limbah Budidaya Pendederan Kerapu Bebek (Cromileptesaltivelis) Menggunakan Spirulina sp. Universitas Lampung, Bandar Lampung.
Qurbani, N. P. (2015). Deteksi Kualitas Air Menggunakan Bentos di Sungai Way Sekampung, Metro Kibang Lampung Timur. Universitas Lampung, Bandar Lampung.
Rajasekaran, C., Ajeesh, C. P. M., Balaji S., Shalini, M., Siva, B., Das, R., Fulzele, D. P., & Kalaivani, T. (2016). Effect of Modified Zarrouk's Medium on Growth of Different Spirulina Strains. Walailak Journal of Science & Technology. 13(1): 67-75.
Rinawati, D., Hidayat, R. Suprianto, & P. S.Dewi. (2016). Penentuan Kandungan Zat Padat (Total Dissolve Solid dan Total Suspended Solid) di Perairan Teluk Lampung. Analit: Analytical and Environmental Chemistry.1(1).
Sigiro, P.S., & Rokaya, E. (2016). Efektifitas Penyerapan Timbal (Pb) oleh Bunga Matahari (Helianthus annuus LINN) Menggunakan Penambahan Mikoriza dan Edta. UAJY. Universitas Dipenogoro, Semarang.
Simamora, L. A., Sudarno, & T. Istirokhatun. (2017). Kultivasi Mikroalga Sebagai Metode Pengolahan dalam Menyisihkan Kadar COD dan Amonium pada Limbah Cair Tahu. Jurnal Teknik Lingkungan. 6(1):1-14.
Siregar, A., D. Jubaedah, & M. Wijayanti. (2017). Penggunaan Hydrilla verticillata Sebagai Fitoremediator dalam Pemeliharaan Ikan Patin (Pangasius sp.). Jurnal Akuakultur Rawa Indonesia, 5(1) :70-82
Sudjarwo T, N. Nisyawati, Rossiana, & W. Mangunwardoyo. (2014). The growth of water hyacinth (Eichhorniacrassipes (Mart.) Solms) and water lettuce (Pistia stratiotes L.) in domestic wastewater in wastewater treatment plant (WWTP) Bojongsoang, Bandung, Indonesia. Journal of Biodiversity and Environmental Sciences. 5(4):393–401.
Uebel, L. S., Costa, J. A. V., Olson, A. C., & Morais, M. G. (2019). Industrial Plant For Production Of Spirulina sp. LEB 18. Brazilian Journal of Chemical Engineering. 36(1).
Wahyuni, N., E. D. Masithah, W. Soemarjati, Suciyono, & M. F. Ulkhaq. (2018). Pola Pertumbuhan Mikroalga Spirulina sp. Skala Laboratorium yang Dikultur Menggunakan Wadah yang Berbeda. Majalah Ilmiah Bahari Jogja, 16(2): 89-97.
Wijaya, H. K. (2009). Komunitas Perifiton dan Fitoplankton Serta Parameter Fisika Kimia Perairan Sebagai Penentu Kualitas Air di Bagian Hulu Sungai Cisadane, Jawa Barat. Institut Pertanian Bogor, Bogor.