The effect of addition of melon (Cucumis melo L.) flesh juice into Lactated Ringer's-egg yolk extender on spermatozoa plasma membrane integrity and spermatozoa morphological abnormalities of semen of native rooster
Downloads
This study aims to determine the effect of adding melon (Cucumis melo L.) juice to Lactated Ringer's-egg yolk extender on the intact plasma membrane (IPM) and morphological abnormalities of native rooster spermatozoa. Five ejaculate each from two native roosters (Gallus gallus domesticus) were divided into four equal volumes. Ejaculate was extended 1:10 (v/v) in Lactated Ringer's-egg yolk without or with the addition of of 2, 4, and 8% (v/v) melon flesh juice, respectively for groups T0, T1, T2, and T3. Extended semen was stored in a refrigerator (5°C) and evaluated every two hours. The results showed that IPM of all groups decreased (p <0.05) consistently, lasting more than 40% for six hours in the T0 and T1 groups and four hours in the T2 and T3 groups. The percentage of spermatozoa morphological abnormalities in all groups stored for six hours was higher (p <0.05) than before storage. However, it was not significantly different (p >0.05) from that which had been stored for two and four hours. It was concluded that native rooster semen extended in Lactated Ringer's-egg yolk without (group T0) or with 2% melon flesh juice (group T1) and stored at 5°C retained spermatozoa plasma membrane integrity of more than 40% and morphological abnormalities of less than 20% for six hours. While the addition of 4 % (T2) and 8% (T3) melon flesh juice maintained the percentage of IPM and spermatozoa morphological abnormalities for only four hours.
Agarwal A, Virk G, Ong C, du Plessis SS. 2014. Effect of oxidative stress on male reproduction. World J Mens Health 32: 1-17.
Akbari A, Jelodar G, Nazifi S, Sajedianfard J. 2016. An overview of the characteristics and function of vitamin C in various tissues: Relying on its antioxidant function. Zahedan J Res Med Sci. 18: e4037.
Ardhani F, Manullang JR, Boangmanalu BM. 2019. Abnormalitas ayam Nunukan asal ejakulat. Jurnal Pertanian Terpadu 7: 122-31.
Asmawarati W, Ismaya I, Yuwanta T. 2010. The Effect of adding vitamin C and E in native chicken semen extender storaged at temperature 4°C on Semen quality and egg fertility. The 5th International Seminar on Tropical Animal Production Community Empowerment and Tropical Animal Industry. October 19-22, 2010, Yogyakarta, Indonesia.
Azzam AH, Raharjo D, Hernawati T, Madyawati SP, Utomo B. 2022. Combination of 5% Dextrose Ringer's solution and egg yolk extender maintained the motility and viability of kampung rooster spermatozoa in chilled temperature. Ovozoa : J Anim Reprod. 11: 109-14.
Danang DR, Isnaini N, Trisunuwati P. 2012. Pengaruh lama simpan semen terhadap kualitas spermatozoa ayam kampung dalam pengencer Ringer's pada suhu 4 ºC. Jurnal Ternak Tropika 13: 47-57.
Evana E, Barek M. 2021. Determination of vitamin C (Ascorbic Acid) contents in two varieties of Melon fruits (Cucumis melo L.) by Iodometric titration. Fullerene J Chem. 6: 143-7.
Feyisa SG, Park YH, Kim YM, Lee BR, Jung KM, Choi SB, Cho CY, Han JY. 2017. Morphological defects of sperm and their association with motility, fertility, and hatchability in four Korean native chicken breeds. Asian-Australas J Anim Sci. 31: 1160-8.
Getachew T. 2016. A review article of artificial insemination in poultry. World's Veterinary Journal 6: 26-35.
Hermiz HN, Shaker AS, Hasafa BMA, Al-Khatib TR, Sardary SY, Toma JS. 2016. Evaluation semen characterization of roosters resulted from different local lines and their crosses with ISA brown. Int J Agric Sci. 1: 7-14.
Junaedi J, Husnaeni H. 2019. Comparative study on the quality of fresh semen of four genetic local chicken in Indonesia. Jurnal Veteriner 20: 397-402.
Kharayat NS, Chaudhary GR, Katiyar R, Balmurugan B, Patel M, Uniyal S, Raza M, Mishra GK. 2016. Significance of artificial insemination in poultry. Res Rev J Vet Sci Technol. 5: 1-5.
Kowalczyk A. 2021. The Role of the natural antioxidant mechanism in sperm cells. Reprod Sci. 29: 1387-94.
Kusumawati ED, Naga BH, Susanto WE. 2019. Kualitas spermatozoa ayam kampung pada suhu 5°C dengan lama simpan yang berbeda. Jurnal Sains Peternakan 7: 141-7.
Kusumawati ED, Krinaningsih ATN, Walangara AUK. 2020. Kualitas spermatozoa ayam kampung dan ayam Arab dengan lama simpan yang berbeda pada suhu ruang. Jurnal Sains Peternakan 8: 41-56.
Malathi G, Vadivelu J, Rajeshkumar S. 2021. Antioxidant revival of Cucumis melo L. modulates the oxidant-antioxidant status on Chromium VI toxicity induced male albino rats. Indian J Sci Technol. 14: 2815-25.
Malik A, Shahdan M, Zakir MI, Sasongko N, Sakiman S. 2018. The addition of fish oil into skim milk-egg yolk extender on the sperm motility and abnormalities post thawed of native chicken. Jurnal Ilmiah Ilmu-Ilmu Peternakan 21: 55-69.
Mallek-Ayadi S, Bahloul N, Baklouti S, Kechaou N. 2022. Bioactive compounds from Cucumis melo L. fruits as potential nutraceutical food ingredients and juice processing using membrane technology. Food Sci Nutr. 10: 2922-34.
Martemucci G, Costagliola C, Mariano M, D'andrea L, Napolitano P, D'Alessandro AG. 2022. Free radical properties, source and targets, antioxidant consumption and health. Oxygen 2: 48-78.
McGary S, Estevez I, Bakst MR. 2003. Potential relationships between physical traits and male broiler breeder fertility. Poult Sci. 82: 328-37.
MeÅ¡Äić Macan A, Gazivoda Kraljević T, Raić-Malić S. 2019. Therapeutic perspective of vitamin C and its derivatives. Antioxidants (Basel) 8: 247.
Mishra AK, Kumar A, Swain DK, Yadav S, Nigam R. 2018. Insights into pH regulatory mechanisms in mediating spermatozoa functions. Vet World. 11: 852-8.
Mussa NJ, Boonkum W, Chankitisakul V. 2023. Semen quality traits of two Thai native chickens producing a high and a low of semen volumes. Vet Sci. 10: 73.
Najafi A, Daghigh-Kia H, Mehdipour M, Mohammadi H, Hamishehkar H. 2021. Comparing the effect of rooster semen extender supplemented with gamma-oryzanol and its nano form on post-thaw sperm quality and fertility. Poult Sci. 101: 101637.
Partyka A, Niżański W. 2021. Supplementation of avian semen extenders with antioxidants to improve semen quality-Is it an effective strategy? Antioxidants (Basel) 10: 1927.
Putranto HD, Nurmeiliasari N, Harferry KT. 2020. Studi kualitas semen ayam Burgo. Buletin Peternakan Tropis 1: 10-5.
Silvestre MA, Yániz JL, Peña FJ, Santolaria P, Castelló-Ruiz M. 2021. Role of Antioxidants in cooled liquid storage of mammal spermatozoa. Antioxidants (Basel) 10: 1096.
Sotler R, Poljšak B, Dahmane R, Jukić T, Pavan Jukić D, Rotim C, Trebše P, Starc A. 2019. Prooxidant activities of antioxidants and their impact on health. Acta Clin Croat. 58: 726-36.
Shanmugam M, Vinoth A, Rajaravindra KS, Rajkumar U. 2014. Evaluation of semen quality in roosters of different age during hot climatic condition. Anim Reprod Sci. 145: 81-5.
Tabatabaei S. 2012. Effect of ascorbic acid on chicken semen quality during liquid storage. Comp Clin Pathol. 21: 621-6.
VaÅ¡íÄek J, Chrenek P. 2013. Effect of storage temperature on the motility characteristics of rooster spermatozoa. J Microbiol Biotechnol Food Sci. 2: 1685-91.
Woli SL, Kusumawati ED, Krisnaningsih ATN. 2017. Motilitas dan viabilitas spermatozoa ayam kampung pada suhu 5°C menggunakan pengencer dan lama simpan yang berbeda. Jurnal Sains Peternakan 5: 138-44.
Yaman MA, Reza MA, Abdullah AN, Usman Y, Hendra Koesmara H. 2022. Sperm quality of hybrid chicken affected by propolis, honey, or royal jelly as organic diluent materials and storage periods during sperm preservation. Adv Biol Sci Res. 20: 206-12.
Zong Y, Li Y, Sun Y, Mehaisen GMK, Ma T, Chen J. 2023. Chicken sperm cryopreservation: Review of techniques, freezing damage, and freezability mechanisms. Agriculture 13: 445.
Copyright (c) 2023 Dinda Galuh Pitaloka, Mas'ud Hariadi, Suherni Susilowati, Budi Utomo, Tatik Hernawati, Eka Pramyrtha Hestianah, Samuel Inioluwa Akeju
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Ovozoa by Unair is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1. The journal allows the author to hold the copyright of the article without restrictions.
2. The journal allows the author(s) to retain publishing rights without restrictions
3. The legal formal aspect of journal publication accessibility refers to Creative Commons Attribution Share-Alike (CC BY-SA).
4. The Creative Commons Attribution Share-Alike (CC BY-SA) license allows re-distribution and re-use of a licensed work on the conditions that the creator is appropriately credited and that any derivative work is made available under "the same, similar or a compatible license”. Other than the conditions mentioned above, the editorial board is not responsible for copyright violation.
