Morphological Identification of Bolbosoma turbinella (Acanthocephala) in Sei Whale (Balaenoptera borealis) from Straits of Malacca, Malaysia

Mohd Ihwan Zakariah, Mohd Tamimi Ali Ahmad, Mohd Shafiq Razak, Norainy Mohd Husin, Wahidah Wahab, Rabi-Atun Adawiah Abdullah, Nor Asma Husna Yusoff, Surzanne Agos, Marina Hassan

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  1. Identification of Morphological characteristic of the Bolbosoma sp.
  2. Genetic confirmation of the specific species of Bolbosoma turbinella.
  3. The first report in Straits of Malacca.
  4. Health status of Sei Whale (Balaenoptera borealis).


The study of ectoparasite and endoparasites of marine mammals are not habitually done because some species are endangered and protected by law. A stranded Sei Whale, Balaenoptera borealis (Anderson, 1878) from the East Coast of Peninsular Malaysia was examined for endoparasites. The objective of this study is to identify the species of acanthocephalan in the intestine of the Sei Whale found in the straits of Malacca. A total of ten parasite specimens were collected from the fresh intestine, and were fixed in 10% buffered formalin for further histological procedures. The morphological features of this parasite viewed under Scanning Electron Microscope (SEM) are referred to as the proboscis armature and variations in the spination of the area between the anterior and posterior cephalic bulb. Genomic DNA extracted by using QIAGEN DNeasy Blood & Tissue Kit DNA and sequenced with First Base Sequencer showed that this species belonged to Bolbosoma turbinella. This was the first record of a sei whale carrying the endoparasites Bolbosoma turbinella, in Malaysian waters.


Sei Whale, Balaenoptera borealis, Endoparasites, Bolbosoma turbinella, Genetic Identification, Morphological study

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Bass, D., Stentiford, G. D., Littlewood, D. T. J., & Hartikainen, H. (2015). Diverse applications of environmental DNA method in parasitology. Trends in Parasitology, 31(10):499-513.

Baylis, H. A. (1932). A list of worms parasitic in Cetacea. Discovery Reports, 6:26.

Costa, G., Chubb, J., & Veltkamp, C. (2000). Cystacanths of Bolbosoma vasculosum in the black scabbard fish Aphanopus carbo, oceanic horse mackerel Trachurus picturatus and common dolphin Delphinus delphis from Madeira, Portugal. Journal of Helminthology, 74(2):113-120.

Delyamure, S. L. (1955). Helminthofauna of marine mammals (Ecology and Phylogeny). Moscow: Izdatel’stvo Akiademii Nauk SSSR.

Diaz-Delgado, J., Fernandez, A., Sierra, E., Sacchini, S., Andrada, M., Vela, A. I., Quesada-Canales, O., Paz, Y., Zucca, D., Groch, K., & Arbelo, M. (2018). Pathologic findings and causes of death of stranded cetaceans in the Canary Islands (2006-2012). Plos One, 13(10):1-33.

Diesing, K. M. (1851). Systema Helminthum. Vol. 2. Berlin: Vindobonae Apud Wilhelmum Braumuller. pp. 558.

Golvan, Y. J. (1959). Acanthocephala of the genus Corynosoma Luhe 1904, mammalian parasites from Alaska & Midway. Annales Parasitology Human Comparison, 34:288-321.

Gregori, M., Aznar F. J., Abollo E., Roura Á., González Á. F., Pascual S. (2012). Nyctiphanes couchii as intermediate host for the acanthocephalan Bolbosoma balaenae in temperate waters of the NE Atlantic. Diseases of Aquatic Organisms, 99:37-47.

Gubanov, N. M. (1951). Giant nematode from the placenta of Cetacea; Placentonema gigantissima nov. gen., nov. sp. Doklady Akademii Nauk SSSR, 77(6):1123-1125.

Hasegawa, M., Kishino, H. & Yano, Taka-aki. (1985). Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. Journal of Molecular Evolution, 22:160–174.

Hermosilla, C., Silva, L. M., Prieto, R., Kleinertz, S., Taubert, A., & Silva, M. A. (2015). Endo- and ectoparasites of large whales (Cetartiodactyla: Balaenopteridae, Physeteridae): Overcoming difficulties in obtaining appropriate samples by non- and minimally-invasive methods. International Journal for Parasitology: Parasites and wildlife, 4(3):414–420.

Ionita, M. M. G., Varela E. T., Lysons. T. R., Spraker. S. C., & Tolliver, S.C. (2008). Hookworms (Uncinaria lucari) and Acanthocephalans (Corynosoma spp. and Bolbosoma spp.) found in dead northern fur seals (Callorhinus ursinus) on St. Paul Island, Alaska in 2007. Parasitology Research, 103(5):1025-1029.

Jafferson, T. A., Webber, M. A. & Pitman, R. L. (2015). Marine mammals of the world: a comprehensive guide to their identification. (2nd Ed.). San Diego: Academic Press.

Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution, 35(6):1547-1549.

Margolis, L., & Pike, G. C. (1955). Some helminth parasites of Canadian Pacific whales. Journal of the Fisheries Board of Canada, 12:97-120.

Measures, M. L. (1992). Bolbosoma turbinella (Acanthocephala) in a Blue Whale, Balaenoptera musculus, stranded in the St. Lawrence Estuary, Quebec. Journal of the Helminthological Society of Washington, 59(2):206-221.

Norina, L., Norsharina, A., Nurnadiah, A. H., Sarol, K., Nurliyana, M. T., Fahmi, Aida, M., Noordin, M. M., Nor-Yasmin, A. R., Hassan, M. D., Kamaruddin, I., Munir, M. N., Syed Abdullah, S. A. K., & Tamimi, M. A. A. (2017). First report of decompression sickness (DCS) in a Sei Whale (Balaenoptera borealis) stranded in Southern Peninsular Malaysia. Malaysian Journal of Veterinary Research, 8:57-64.

Omar, M. A., & Leo, M. (1998). Redescription of Bolbosoma capitatum (Acanthocephala: Polymophidae) from False Killer Whale off Vancouver Island, with taxonomic reconsideration of the species and synonymy of B. physeteris. Journal of the Helminthological Society of Washington, 65(2):179-188.

Shuko, K., Mizuki S., Kentaro G., Ryo M., Hiroshi K., Minoru N., & Hideo, H. (2019). A case small bowel obstruction due to infection with Bolbosoma sp. (Acathocephala: Polymorphidae). Journal of Parasitology International, 68:14-16.

Tada, I., Otsuji, Y., Kmaiya, H., Mimori, T., Sakaguchi, Y., & Makazumi, S. (1983). The first case of a human infected with an Acanthocephalan parasite, Bolbosoma sp. Journal of Parasitology, 69:205-208.

Vongpakorn, M., Pongsopawijit, P., Manawatthana, S., Soison, O., Adulyanukosol, K., Kittiwattanawong, K., and Boontong, P. (2012, May). Identification of helminths in dolphins stranded along the Andaman coast of Thailand during 1992 – 2006. Paper presented at Conference: Thailand-Japan Joint Conference on Animal Health 2012: The 25th Year Anniversary of National Institute of Animal Health, Bangkok, Thailand.

Yamaguti, S. (1963). Systema helminthum. Volume 5, Acenthocephala. New York: Interscience Publishers.


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