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Pemeriksaan Viral Nervous Necrosis (VNN) Pada Ikan dengan Metode Polymerase Chain Reaction (PCR)
[Viral Nervous Necrosis (VNN) Examination at Fish by Polymerase Chain Reaction (PCR) Method]
Corresponding Author(s) : Abdul Manan
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
Fish production annually has increased. Trading activities of Fishery in Indonesia both exports and imports will continue to increase every year. Control and monitoring of fisheries products was transported in Indonesia aims to reduce the risk of entry and spread of pests and diseases of fish in Indonesia. The purpose of Study is to know examination techniques of virus Viral Nervous Necrosis (VNN) at fish by Polymerase Chain Reaction (PCR) method. This Study was held in the Quarantine Fish Quality Control and Safety of Fishery Products (BBKIPM-KHP) Soekarno Hatta Tangerang, Banten on January 12th to February 12th, 2015. The methods of data fetching consisted of primary and secondary data. The data fetching was performed with active participation, observation, interviews, and literature. Examination methods Viral Nervous Necrosis (VNN) by Polymerase Chain Reaction (PCR). Stages of virus examination by PCR method includes necropsy samples, RNA extraction, amplification, electrophoresis, and the diagnosis results using UV transluminator. Fish samples were examined VNN virus consisting of a live fish and frozen fish both commodity exports, imports, or domestic. The results showed that all fish samples are negative infected with the virus VNN.
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- Keputusan Menteri Kelautan Dan Perikanan Republik Indonesia Nomor 26 Tahun 2013 Tentang tentang Penetapan JenisJenis Hama Dan Penyakit Ikan Karantina, Golongan, Media Pembawa, Dan Sebarannya. Jakarta.
- Langga, I. F., M. Restu, dan T. Kuswinanti. 2012. Optimalisasi Suhu dan Lama Inkubasi dalam Ekstraksi DNA Tanaman Bitti (Vitex cofassus Reinw) serta Analisis Keragaman Genetik dengan Teknik RAPD-PCR. J. Sains & Teknologi. Vol.12 (3) : 265 – 276.
- Munday B.L., Kwang J. dan Moody N. 2002. Betanodavirus infections of teleost fish: a review. Jurnal of Fish Diseases Vol. 25: 127–142.
- Pelczar, M.J. dan E.C.S Chan. 1988. Dasar – Dasar Mikrobiologi 2. UI-Press. Jakarta. Hal 461-467
- Seow V. L and A.R. Bahaman. 2012. Discriminatory Power of Agarose Gel Electrophoresis in DNA Fragments Analysis. In: Magdeldin S. Gel Electrophoresis – Principles And Basics. In Tech. Croatia. Hal 41-56
- Sugianti, B. 2005. Pemanfaatan Tumbuhan Obat Tradisional Dalam Pengendalian Penyakit Ikan. Makalah Falsafah Sains. Institut Pertanian Bogor. Bogor. 37 hal.
- Srigede L. dan S. Zaetun. 2014. Paparan Sinar Ultra Violet (UV) dengan Pengamatan Waktu Sterilisasi terhadap Pertumbuhan Bakteri Bacillus sp. Media Bina Ilmiah Vol. 8 (6) : 75-79.
- Verkuil E.V., A. van Belkum, dan J. P. Hays. 2008. Principles and Technical Aspects of PCR Amplification. Netherlands : Springer Science + Business Media B. V.
- Yusuf, Z. K. 2010. Polymerase Chain Reaction (PCR). Saintek Vol.5 (6) : 1-6.
References
Keputusan Menteri Kelautan Dan Perikanan Republik Indonesia Nomor 26 Tahun 2013 Tentang tentang Penetapan JenisJenis Hama Dan Penyakit Ikan Karantina, Golongan, Media Pembawa, Dan Sebarannya. Jakarta.
Langga, I. F., M. Restu, dan T. Kuswinanti. 2012. Optimalisasi Suhu dan Lama Inkubasi dalam Ekstraksi DNA Tanaman Bitti (Vitex cofassus Reinw) serta Analisis Keragaman Genetik dengan Teknik RAPD-PCR. J. Sains & Teknologi. Vol.12 (3) : 265 – 276.
Munday B.L., Kwang J. dan Moody N. 2002. Betanodavirus infections of teleost fish: a review. Jurnal of Fish Diseases Vol. 25: 127–142.
Pelczar, M.J. dan E.C.S Chan. 1988. Dasar – Dasar Mikrobiologi 2. UI-Press. Jakarta. Hal 461-467
Seow V. L and A.R. Bahaman. 2012. Discriminatory Power of Agarose Gel Electrophoresis in DNA Fragments Analysis. In: Magdeldin S. Gel Electrophoresis – Principles And Basics. In Tech. Croatia. Hal 41-56
Sugianti, B. 2005. Pemanfaatan Tumbuhan Obat Tradisional Dalam Pengendalian Penyakit Ikan. Makalah Falsafah Sains. Institut Pertanian Bogor. Bogor. 37 hal.
Srigede L. dan S. Zaetun. 2014. Paparan Sinar Ultra Violet (UV) dengan Pengamatan Waktu Sterilisasi terhadap Pertumbuhan Bakteri Bacillus sp. Media Bina Ilmiah Vol. 8 (6) : 75-79.
Verkuil E.V., A. van Belkum, dan J. P. Hays. 2008. Principles and Technical Aspects of PCR Amplification. Netherlands : Springer Science + Business Media B. V.
Yusuf, Z. K. 2010. Polymerase Chain Reaction (PCR). Saintek Vol.5 (6) : 1-6.