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Teknik Dasar Histologi pada Ikan Gurami (Osphronemus gouramy)
[ The Basic Histology Technique of Gouramy Fish (Osphronemus gourami)]
Corresponding Author(s) : Abdul Manan
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
Histology is science that learns about cell,organ, and body tissues in a microscopic condition. Whereas science that learns about morbidity or patology of a tissue that's called as histopatology. Both of normal tissue's structure and abnormal tissue's stucturecan be learned by microscopic in a tissue preparation. This preparation made through processing of tissue until the preparation coloured. Then histology's structure can be watched clearly so that make it easy to read. Field Work Practice purpose (PKL) this is to know the basic histoloy technique in fish. This Field Work Practice was held in Balai Uji Standar Karantina Ikan Pengendalian Mutu dan Keamanan Hasil Perikanan (BUSKIPM) Jl. Harapan I No. 01A Cilangkap, East Jakarta on 14th January 2013 until 14th February 2013. The method of this Field Work Practice is descriptive method by collecting data through primary data and secondary data. The histology technique in fish are tissue fixation, trimming (tissue selection), tissue dehydration, the tissue block making, tissue cutting, tissue colouring, and tissue observation. From the result got from the gourami's tissue, there are edema and infestation of metacercaria parasites and myxosporidia in gill normal intestine tissue, normal liver tissue, and normal spleen tissue
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- Genten, F., Terwinghe, E and Danguy, A., 2009. Atlas of Fish Histology. Science Publishers, Enfield, NH, USA. Departemen of Histology and Biopathology of Fish Fauna Laboratory of Functional Morphology, Universite Libre de Bruxelles (U.L.B), Brussel.
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References
Genten, F., Terwinghe, E and Danguy, A., 2009. Atlas of Fish Histology. Science Publishers, Enfield, NH, USA. Departemen of Histology and Biopathology of Fish Fauna Laboratory of Functional Morphology, Universite Libre de Bruxelles (U.L.B), Brussel.
Junqueira, C.L and Carneiro, J. 2005. Basic Histology text and atlas. Eleventh edition. United States of America. New York.
Jusuf, A.A. 2009. Histoteknik Dasar. Bagian Histologi Fakultas Kedokteran. Universitas Indonesia. Jakarta. Kurniasih. 2008. Histopatologi Ikan. Apresiasi Balai Uji Standard Karantina Ikan. Pusat Karantina Ikan. Jakarta.
Nuryadin, 2010. Pola Larik Indukan Gurami yang resisten Terhadap Bakteri Aeromonas hydrophila dengan pola larik DNA sampel. UPI : Bandung. http://repository.upi.edu. Diakses pada: 1 Juni 2013.
Panigoro, N., E. Astuti I., Bahnan, M., Prayudha, DC., Salfira dan Wakita, K. 2007. Teknik Dasar Histology dan Atlas Dasar-dasar Histopatologi Ikan. Balai Budidaya Air Tawar Jambi dan Japan International Cooperation Agenci. Jambi.
Perceka, M. L. 2011. Analisis Deskriptif Kemunduran Mutu Kulit Ikan Bandeng (Chanos chanos) Selama Penyimpanan Suhu Chilling Melalui Pengamatan Histologis. Skripsi. Institut Pertanian Bogor. Bogor. 52hal.
Purnomo, R., Hartono, P., dan Nirasari. 2002. Pengelolaan Kesehatan Ikan Budidaya Laut. Balai Budidaya Laut Lampung.
Rahmana, P., Uun, Y dan Asus, M., 2013. Perubahan Struktur Mata Dan Otak Pada Larva Ikan Kerapu Tikus (Cromileptes altivelis) Yang Terinfeksi Viral Nervous Necrosis (VNN) Dengan Pemeriksaan Scanning Microscope (SEM). Student Journal. Vol.I No.1 pp1-10. Universitas Brawijaya.