Karakterisasi Protein Lernaea Cyprinacea Dengan Metode Elektroforesis SDS-PAGE
[Characterization Of Protein Lernaea cyprinacea By Using SDS-PAGE Electrophoresis Method]

Gunanti Mahasri, Ulia Fajriah, Sri Subekti

= http://dx.doi.org/10.20473/jipk.v2i1.11669
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Abstract


Abstract

One of the diseases that often attack are fish is parasitic disease caused by Lernaea cyprinacea, called Lernaeosis. This ectoparasites could be detected on skin, gill, eyes, fin or inside of mouth and nostril of fishes. The aim of the study was to know the character is tic of L.cyprinacea protein based on molecular weight using SDS-PAGE. The results of this study were expected as Lernaeosis vaccine candidate. The experiment used SDS-PAGE with 12% separating gel, 3% stacking gel, voltage 110 volts 28 A and gel staining using coomassie blue. The result showed that, there were eight protein bands with molecular weight 82.3, 73.3, 66.6, 60.5, 54.9, 27.5, 23.1 and 19.8 kDa. Among them, the bands with molecular weight 27.5, 23.1 and 19.8 kDa were thicker and clearer than the others.


Keywords


Lernaeosis, SDS-PAGE, protein

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References


Achjar, M dan Rismunandar. 2003. Perikanan Darat. Bandung: Sinar Baru Algensindo. Hal 89–92.

de Magalhaes, A.L.B., 2006. First Record of Lernaeosis in a Native Fish Species from a Natural Environment in Minas Gerais State, Brazil. Pan-American Aquatic Scie., 1 (1): 8– 10.

Handajani, H. dan S. Samsundari. 2005. Parasit dan Penyakit Ikan. UMM Press. Malang. Hal 83 – 90, 191 – 195.

Hardjamulia, A. 1976. Problem in Freshwater Fish Breeding and the Results of the Introduction of Improved Techniques in Indonesia. Research Institude for Inland Fisheries, Sukabumi, Indonesia. http://www.apific.org/ Accessed April, 25, 2008. pp 313 – 324.

Kabata, Z., 1985. Parasites and Diseases of Fish Cultured in the Tropics. London and Philadelphia: Taylor & Francis. Hal 227 –242.

Karantina Ikan Kupang. 2004a. Laporan Pemantauan Daerah Sebar Hama dan Penyakit Ikan Karantina Tahun Anggaran 2004 Lingkup Pos Karantina Ikan El Tari Kupang. Pos Karantina Ikan El Tari Kupang, Pusat Karantina Ikan Departemen Kelautan dan Perikanan. 26 hal.

Karantina Ikan Ngurah Rai. 2004b. Laporan Pemantauan Hama dan Penyakit Ikan Karantina di Propinsi Bali Tahun Anggaran 2004. Stasiun Karantina Ikan Kelas I Ngurah Rai-Bali. 66 hal.

Karantina Ikan Pangkalpinang. 2004c. Laporan Pema n t a u a n Po s Ka r a n t i n a I k a n Pangkalpinang Tahun 2004. Pusat Karantina Ikan Departemen Kelautan dan Perikanan. 30 hal.

Kindlmann, P.J. and R.A. Valley. 1991. High Voltage Electrophoresis Apparatus. Eastman Kodak C o m p a n y ( R o c h e s t e r , N Y ) . http://www.free.patentsononline.com. Accessed September, 2008. 5 pp.

Lastuti, N.D.R., F.J. Wibisono dan R. Sidiq. 2006. Identifikasi Profil Protein Sarcoptes scabiei pada Kambing dengan Analisis SDS-PAGE. Su r a b a y a : u n i v e r s i t a s Ai r l a n g g a .http://www.jurnal.unair.ac.id. 6 hal.

Mahasri, G. 2007. Protein Membran Imunogenik Zoothamnium penaei sebagai Bahan Pengembangan Imunostimulan pada Udang Windu (Penaeus monodon Fabricus) terhadap Zoothamniosis. Disertasi. Program Pascasarjana Universitas Airlangga. Surabaya.

Nagasawa, K., A. Inoue, Su Myat and T. Umino. 2007. New Host Records for Lernaea cyprinacea (Copepoda), a Parasite of Freshwater Fishes, with a Checklist of the Lernaeidae in Japan (1915-2007). Japan: Hiroshima University, J. Grad. Sch. Biosp. Sci. 46: 21 – 33.

Narbuko, C., dan A. Achmadi 2007. Metodologi Penelitian. Jakarta: Bumi Aksara. Hal 44. Perez-Bote, J. L., 2005. First Record of Lernaea cyprinacea (Copepoda: Cyclopoida) on the Allis Shad. Folia boilogica (Krakow) Spain, 53 (3 – 4): 197 – 198.

Rantam, F. A. 2003. Metode Imunologi. Surabaya. Airlangga University Press.hal 145 – 162.

Scopes, R. K. 1993. Protein Purification Principles and Practice, Third Edition. New York: Springervertag.

Tizard, I. R. 1987. Pengantar Imunologi Veteriner. Penerjemah oleh: M. Partodiredjo. Airlangga University Press. Universitas Airlangga. Surabaya. Hal. 237 – 255.

Waluyo, J. 2004. Purifikasi dan Karakterisasi Protein Antibakteri dari Cacing Tanah. Desertasi. Program Pascasarjana Universitas Airlangga. Surabaya.


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