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Effects of Different Acetic Acid Immersion Time on the Properties of Collagen from Pangasius Skin
Corresponding Author(s) : Patmawati
Jurnal Ilmiah Perikanan dan Kelautan,
Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Highlight Research
- Experimental use of pre-treated pangasius skin as collagen raw material using different acetic acid immersion time
- Effects of immersion time on the yield of collagen from pangasius skin
- Effects of immersion time on the proximate content of collagen from pangasius skin
- Presenting SDS-Page profile of collagen from pangasius skin
Abstract
Pangasius sp. production in Indonesia has reached 384,310 tons in 2019. One of the main waste in pangasius fillet industry is the skin, which reached to 6% of body mass. Fish skins are alternative for making collagen because it has no restrictions for certain religions and ethnicities. Collagen is a protein biomaterial which acts as the main component of connective tissue. Extracting collagen using organic acids such as acetic acid is the most common extraction method. Herein, we report the effects of different immersion time of acetic acid to collagen properties from pangasius skin. In this study, pangasius skin was treated using 10% alcohol with the ratio 1:10 (w/v) for defatting and 0.1M NaOH with the ratio 1:10 (w/v) for eliminating non-collagenous protein. The immersion of 0.5M acetic acid was carried out on pangasius skin with the ratio of 1:20 (w/v), for 24, 48, and 72h at temperature less than 15oC. The sample was salted-out for 24 hours with NaCl until the concentration reached 2.5M, then the sample was freeze-dried at -40oC. This study investigated that longer immersion time affected the properties of pangasius skin collagen. Different immersion time significantly affected the yield of collagen from pangasius skin (p<0.05). Pangasius skin has a potential to be used as collagen raw material, and 72h of immersion time (P3) is suggested to produce pangasius skin collagen with the highest yield and protein content (6.15 % and 9.26 %). Production of collagen from the fish skin will contribute to increase pangasius waste valorization in pharmaceutical industry.
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- Ahmad, T., Ismail, A., Ahmad, S. A., Khalil, K. A., Kee, L. T, Awad, E. A., & Sazili, A. Q. (2019). Physicochemical characteristics and molecular structures of gelatin extracted from bovine skin: effects of actinidin and papain enzymes pretreatment. International Journal of Food Properties, 22(1):138-153.
- Azizah, N., Ochiai, Y., & Nurilmala, M. (2020). Collagen peptides from Pangasius fish skin as antioxidants. IOP Conference Series: Earth and Environmental Science, 404(1):012055.
- Choi, J. H., Behnam, Sh., & Kim, S. M. (2013). Physico-biochemical characteristics of scallop mantle collagen soluble in pepsin. Journal of Agricultural Science and Technology, 15(2):293-302.
- Devita, L., Nurilmala, M., Lioe, H. N., & Suhartono, M. T. (2021). Chemical and antioxidant characteristics of skin-derived collagen obtained by acid-enzymatic hydrolysis of bigeye tuna (Thunnus obesus). Marine Drugs, 19(4):222.
- Draelos, Z. D., & Thaman L. A. (2006). Cosmetic Formulation of Skin Care Products (Cosmetic Science and Technology Series, Vol 30). New York: Taylor and Francis Group.
- Fabella, N., Herpandai, H., & Widiastuti, I. (2018). Pengaruh metode ekstraksi terhadap karakteristik kolagen dari kulit ikan patin (Pangasius sp.). Jurnal Fishtech, 7(1):69-75.
- Falleh, H., Ksouri, R., Lucchessi, M. E., Abdelly, C., & Magné, C. (2012). Ultrasound-assisted extraction: Effect of extraction time and solvent power on the levels of polyphenols and antioxidant activity of Mesembryanthemum edule L. Aizoaceae shoots. Tropical Journal of Pharmaceutical Research, 11(2):243-249.
- Jaswir, I., Monsur, H. A., & Salleh, H. M. (2011). Nano-structural analysis of fish collagen extracts for new process development. African Journal of Biotechnology, 10(81):18847-18854.
- KKP (Kementrian Kelautan dan Perikanan). (2021). Pengolahan data produksi kelautan dan perikanan. Jakarta: Pusat Data Statistik dan Informasi Kementerian Kelautan dan Perikanan (KKP) RI.
- Kim, H. K., Kim, Y. H., Park, H. J., & Lee, N. H. (2013). Application of ultrasonic treatment to extraction of collagen from the skins of sea bass Lateolabrax japonicus. Fisheries Science, 79(5):849-856.
- Kurien, B. T., & Scofield, R. H. (2012). Common artifacts and mistakes made in electrophoresis. In B. T. Kurien, and R. H. Scofield (Ed.), Protein electrophoresis. (pp. 633-640). New Jersey: Humana Press.
- Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259):680-685.
- Lee, C. H., Singla, A., & Lee, Y. (2001). Biomedical applications of collagen. International Journal of Pharmaceutics, 221(1-2):1-22.
- Mahmoodani, F., Ardekani, V. S., Fern, S. S., Yusof, S. M., & Babji, A. S. (2014). Optimization of extraction dan physicochemical properties of gelatin from pengasius pangasius (Pengasius sutchi) skin. Sains Malaysiana, 43(7):995-1002.
- Normah, I., & Afiqah, M. M. (2018). Effect of extraction time on the physico-chemical characteristics of collagen from sin croaker (Johniecop sina) waste. International Food Research Journal, 25(3):1074-1080.
- Nurhayati, T., & Astiana, I. (2018). Characteristics of papain soluble collagen from redbelly yellowtail fusilier (Caesio cuning). IOP Conference Series: Earth and Environmental Science, 196(1):012034.
- Ogawa, M., Portier, R. J., Moody, M. W., Bell, J., Schexnayder, M. A., & Losso, J. N. (2004). Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum (Pogonia cromis) and sheepshead seabream (Archosargus probatocephalus). Food chemistry, 88(4):495-501.
- Sahubawa, L., & Putra, A. N. (2011). Pengaruh konsentrasi asam asetat dan waktu ekstraksi terhadap mutu kolagen kulit nila hitam. Jurnal Teknosains, 1(1):16-25.
- Sasmal, P., & Begam, H. (2014). Extraction of type-I collagen from sea fish and synthesis of hap/collagen composite. Procedia Materials Science, 5:1136-1140.
- Sathivel, S., Witoon, P., Casey, C. G., Joan, M. K., & Steven, L. (2012). Fatty acid composition of crude oil recovered from pangasius viscera. Journal of the American Oil Chemists Society, 79(10):989-992.
- Singh, P., Benjakul, S., Maqsood, S., & Kishimura, H. (2011). Isolation and characterisation of collagen extracted from the skin of striped catfish (Pangasianodon hypophthalmus). Food Chemistry, 124(1):97-105.
- Sonavane, A. E., Koli, J. M., Patange, S. B., Naik, S. D., & Mohite, A. S. (2018). Isolation of acid and pepsin soluble collagens from the skin of Indian mackerel Rastrelliger kanagurta (Cuvier, 1817). Journal of Entomology and Zoology Studies, 6(2):2508-2511.
- Suparno, O., & Prasetyo, N. B. (2019). Isolation of collagen from chicken feet with hydro-extraction method and its physico-chemical characterisation. IOP Conference Series: Earth and Environmental Science, 335(1):012018.
- Suptijah, P., Indriani, D., & Wardoyo, S. E. (2018). Isolation and characterization of collagen from the skin of pangasius (Pangasius sp.). Jurnal Sains Natural, 8(1):8-23.
- Tangkaa, R., Mentang, F., Agustin, A. T., Onibala, H., Kaseger, B. E., Makapedua, D. M., & Sanger, G. (2020). Pengaruh perbedaan konsentrasi asam asetat dan lama ekstraksi kolagen dari kulit ikan situhuk hitam (Makaira indica). Media Teknologi Hasil Perikanan, 8(2):44-49.
- Tabarestani, H. S., Maghsoudlou, Y., Motamedzadegan, A., Mahoonak, A. R., & Rostamzad, H. (2012). Study on some properties of acid-soluble collagens isolated from fish skin and bones of rainbow trout (Onchorhynchus mykiss). International Food Research Journal, 19(1):251-257.
- Wulandari, Suptijah, P., & Tarman, K. (2015). Efektivitas pre-treatment alkali dan hidrolisis asam asetat terhadap karakteristik kolagen dari kulit ikan gabus. Jurnal Pengolahan Hasil Perikanan Indonesia, 18(3): 287-302.
- Yu, D., Chi, C. F., Wang, B., Ding, G. F., & Li, Z. R. (2014). Characterization of acid and pepsin soluble collagens from spines and skulls of skipjack tuna (Katsuwonus pelamis). Chinese Journal of Natural Medicines, 12(9):712-720.
References
Ahmad, T., Ismail, A., Ahmad, S. A., Khalil, K. A., Kee, L. T, Awad, E. A., & Sazili, A. Q. (2019). Physicochemical characteristics and molecular structures of gelatin extracted from bovine skin: effects of actinidin and papain enzymes pretreatment. International Journal of Food Properties, 22(1):138-153.
Azizah, N., Ochiai, Y., & Nurilmala, M. (2020). Collagen peptides from Pangasius fish skin as antioxidants. IOP Conference Series: Earth and Environmental Science, 404(1):012055.
Choi, J. H., Behnam, Sh., & Kim, S. M. (2013). Physico-biochemical characteristics of scallop mantle collagen soluble in pepsin. Journal of Agricultural Science and Technology, 15(2):293-302.
Devita, L., Nurilmala, M., Lioe, H. N., & Suhartono, M. T. (2021). Chemical and antioxidant characteristics of skin-derived collagen obtained by acid-enzymatic hydrolysis of bigeye tuna (Thunnus obesus). Marine Drugs, 19(4):222.
Draelos, Z. D., & Thaman L. A. (2006). Cosmetic Formulation of Skin Care Products (Cosmetic Science and Technology Series, Vol 30). New York: Taylor and Francis Group.
Fabella, N., Herpandai, H., & Widiastuti, I. (2018). Pengaruh metode ekstraksi terhadap karakteristik kolagen dari kulit ikan patin (Pangasius sp.). Jurnal Fishtech, 7(1):69-75.
Falleh, H., Ksouri, R., Lucchessi, M. E., Abdelly, C., & Magné, C. (2012). Ultrasound-assisted extraction: Effect of extraction time and solvent power on the levels of polyphenols and antioxidant activity of Mesembryanthemum edule L. Aizoaceae shoots. Tropical Journal of Pharmaceutical Research, 11(2):243-249.
Jaswir, I., Monsur, H. A., & Salleh, H. M. (2011). Nano-structural analysis of fish collagen extracts for new process development. African Journal of Biotechnology, 10(81):18847-18854.
KKP (Kementrian Kelautan dan Perikanan). (2021). Pengolahan data produksi kelautan dan perikanan. Jakarta: Pusat Data Statistik dan Informasi Kementerian Kelautan dan Perikanan (KKP) RI.
Kim, H. K., Kim, Y. H., Park, H. J., & Lee, N. H. (2013). Application of ultrasonic treatment to extraction of collagen from the skins of sea bass Lateolabrax japonicus. Fisheries Science, 79(5):849-856.
Kurien, B. T., & Scofield, R. H. (2012). Common artifacts and mistakes made in electrophoresis. In B. T. Kurien, and R. H. Scofield (Ed.), Protein electrophoresis. (pp. 633-640). New Jersey: Humana Press.
Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227(5259):680-685.
Lee, C. H., Singla, A., & Lee, Y. (2001). Biomedical applications of collagen. International Journal of Pharmaceutics, 221(1-2):1-22.
Mahmoodani, F., Ardekani, V. S., Fern, S. S., Yusof, S. M., & Babji, A. S. (2014). Optimization of extraction dan physicochemical properties of gelatin from pengasius pangasius (Pengasius sutchi) skin. Sains Malaysiana, 43(7):995-1002.
Normah, I., & Afiqah, M. M. (2018). Effect of extraction time on the physico-chemical characteristics of collagen from sin croaker (Johniecop sina) waste. International Food Research Journal, 25(3):1074-1080.
Nurhayati, T., & Astiana, I. (2018). Characteristics of papain soluble collagen from redbelly yellowtail fusilier (Caesio cuning). IOP Conference Series: Earth and Environmental Science, 196(1):012034.
Ogawa, M., Portier, R. J., Moody, M. W., Bell, J., Schexnayder, M. A., & Losso, J. N. (2004). Biochemical properties of bone and scale collagens isolated from the subtropical fish black drum (Pogonia cromis) and sheepshead seabream (Archosargus probatocephalus). Food chemistry, 88(4):495-501.
Sahubawa, L., & Putra, A. N. (2011). Pengaruh konsentrasi asam asetat dan waktu ekstraksi terhadap mutu kolagen kulit nila hitam. Jurnal Teknosains, 1(1):16-25.
Sasmal, P., & Begam, H. (2014). Extraction of type-I collagen from sea fish and synthesis of hap/collagen composite. Procedia Materials Science, 5:1136-1140.
Sathivel, S., Witoon, P., Casey, C. G., Joan, M. K., & Steven, L. (2012). Fatty acid composition of crude oil recovered from pangasius viscera. Journal of the American Oil Chemists Society, 79(10):989-992.
Singh, P., Benjakul, S., Maqsood, S., & Kishimura, H. (2011). Isolation and characterisation of collagen extracted from the skin of striped catfish (Pangasianodon hypophthalmus). Food Chemistry, 124(1):97-105.
Sonavane, A. E., Koli, J. M., Patange, S. B., Naik, S. D., & Mohite, A. S. (2018). Isolation of acid and pepsin soluble collagens from the skin of Indian mackerel Rastrelliger kanagurta (Cuvier, 1817). Journal of Entomology and Zoology Studies, 6(2):2508-2511.
Suparno, O., & Prasetyo, N. B. (2019). Isolation of collagen from chicken feet with hydro-extraction method and its physico-chemical characterisation. IOP Conference Series: Earth and Environmental Science, 335(1):012018.
Suptijah, P., Indriani, D., & Wardoyo, S. E. (2018). Isolation and characterization of collagen from the skin of pangasius (Pangasius sp.). Jurnal Sains Natural, 8(1):8-23.
Tangkaa, R., Mentang, F., Agustin, A. T., Onibala, H., Kaseger, B. E., Makapedua, D. M., & Sanger, G. (2020). Pengaruh perbedaan konsentrasi asam asetat dan lama ekstraksi kolagen dari kulit ikan situhuk hitam (Makaira indica). Media Teknologi Hasil Perikanan, 8(2):44-49.
Tabarestani, H. S., Maghsoudlou, Y., Motamedzadegan, A., Mahoonak, A. R., & Rostamzad, H. (2012). Study on some properties of acid-soluble collagens isolated from fish skin and bones of rainbow trout (Onchorhynchus mykiss). International Food Research Journal, 19(1):251-257.
Wulandari, Suptijah, P., & Tarman, K. (2015). Efektivitas pre-treatment alkali dan hidrolisis asam asetat terhadap karakteristik kolagen dari kulit ikan gabus. Jurnal Pengolahan Hasil Perikanan Indonesia, 18(3): 287-302.
Yu, D., Chi, C. F., Wang, B., Ding, G. F., & Li, Z. R. (2014). Characterization of acid and pepsin soluble collagens from spines and skulls of skipjack tuna (Katsuwonus pelamis). Chinese Journal of Natural Medicines, 12(9):712-720.