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Isolasi Bakteri Indigen Sebagai Pendegradasi Bahan Organik Pada Media Pembenihan Ikan Lele Dumbo (Clarias sp.) Sistem Resirkulasi Tertutup
[Isolation Indigen Bacteria In Organic Materials As Media Degrading Seedling Fish Catfish (Clarias sp.) Closed Recirculation System]
Corresponding Author(s) : Muhammad Arief
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 2 No. 2 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
Rearing of African catfish fry requires an abundant water supply, so this causes Clarias gariepinus hatchery operations difficult in areas that lack water, so use a closed recirculation system technology which is an attempt to tackle the problem of limited water in an area for hatchery operations. However, these systems have drawbacks which deposition of organic materials on water quality pond maintenance reduced the need for water quality management. Components of organic materials is a source of water quality pollutants consisting of proteins, lekam, and carbohydrates, so that in the organic material needed pendegradasian existence of groups of bacteria and proteolytic bacteria, lipolytic, and amylolitic. The results of this study show that in the media of African catfish hatchery fish have bacteria that degrade organic material that has properties of proteolytic, lipolytic, and most potentially amylolitic consisting of bacterial isolates of Pseudomonas stutzeri B ie (97.81%) with values highest protease hydrolysis test results 1.878 mm, ie G isolates of Pseudomonas pseudomallei (97.81%) with the highest value of 1.909 mm hydrolysis test and bacterial isolates of Pseudomonas stutzeri K (61.21%) with the highest value of 1.944 mm hydrolysis test.
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- Ashdown, L. R. 1979. Identification of Pseudomonas pseudomallei in The Clinical Laboratory. http://www.journal-of-clinical-pathology.com.04/05/2010.5pp.
- Barrow, G.I and R.K.A. Feltham.2002. Cowan and Steels Manual for The Identifikation of Medical Bakteria. Chambridge Uiversity Press.
- Boyd, C.E. 1992. Water Quality In Ponds For Aquaculture. Bermingham Publishing Co Bermingham, Alabama.
- Darmayasa., I. B.G. 2008. Isolasi Dan Identifikasi Bakteri Pendegradasi Lipid (Lemak) Pada Beberapa Tempat Pembuangan Limbah Dan Estuari DAM Denpasar. Jurnal Bumi Lestari, Vol 8. hal 122-127.
- Droste, R.L. 1997. Theory and Practice of Water and Wastewater Treatment. USA. John Wiley & Sons, Inc. hal. 547 -595
- Fardiaz, S. 1993. Analisis Mikrobiologi Pangan. Raja Grafindo Persada. Jakarta.
- Inglis, T.J.J and J.L. Sagripanti. 2006. Environmental Factors that Effect The Survival and Persistance of Burkholderia pseudomallei American Society for Microbiol. 72 (11): 6865-6875. http//aem.asm.org.06/08/2008.
- Irianto, A. 2003. Probiotik Akuakultur. Gadjah Mada University Press. 125 hal.
- Madigan, M.T., V.M. Martinko, and J. Parker. 2003. Biologi of Microorganism. Eighth Edition. Prentince Hall, Inc., New Jersey.
- Prihartono, K, Rasidik, J dan Arie, U. 2000. Mengatasi Permasalahan Budidaya Lele Dumbo. Penebar Swadaya. Jakarta.
- Roberts M., M. E. Mercade, M. P. Bosch, J. L. Parra, M. J. Espuny, M. A. Manresa dan J. Guinea. 1989. Effect of Carbon Source on Biosurfactant Production by Pseudomonas aeruginosa 44T1. Biotech Lett. 11:871-874.
- Saanin, H. 1984. Taksonomi dan Kunci Identifikasi Ikan vol I dan II. Bina Cipta Press. Jakarta.
- Salle, A. J. 1991. Fundamental Principles of Bacteriology Fifth Edition. Mc Graw Hill Book Company. Ney York. 812 p.
- Sasongko, A. 2001. Biomassa Bakteri Nitrifikasi pada Berbagai Bahan Filter dalam Sistem Resirkulasi Aliran Tertutup dan Pengaruhnya terhadap Kondisi Ikan. Program Studi Ilmu Perairan. IPB. 59 hal.
- Sexena, R. K., P. K. Ghost, R. Gupta, W. S. Davidson, S. Bradoo dan R. Gulati. 2004. Microbial Lipase: Potensial Biocatalysts for Tha Future Industry. http:/www.las.ac.in/currsci/jul10/articles18.htl. Akses 13/10/09.
- Suarsini, E. 2006. Bioremediasi Limbah Cair Rumah Tangga menggunakan Konsorsia Bakteri Indigen yang berpotensi Pereduksi Polutan. Disertasi Program Studi Pendidikan Biologi. Universitas Negeri Malang. 211 hal.
References
Ashdown, L. R. 1979. Identification of Pseudomonas pseudomallei in The Clinical Laboratory. http://www.journal-of-clinical-pathology.com.04/05/2010.5pp.
Barrow, G.I and R.K.A. Feltham.2002. Cowan and Steels Manual for The Identifikation of Medical Bakteria. Chambridge Uiversity Press.
Boyd, C.E. 1992. Water Quality In Ponds For Aquaculture. Bermingham Publishing Co Bermingham, Alabama.
Darmayasa., I. B.G. 2008. Isolasi Dan Identifikasi Bakteri Pendegradasi Lipid (Lemak) Pada Beberapa Tempat Pembuangan Limbah Dan Estuari DAM Denpasar. Jurnal Bumi Lestari, Vol 8. hal 122-127.
Droste, R.L. 1997. Theory and Practice of Water and Wastewater Treatment. USA. John Wiley & Sons, Inc. hal. 547 -595
Fardiaz, S. 1993. Analisis Mikrobiologi Pangan. Raja Grafindo Persada. Jakarta.
Inglis, T.J.J and J.L. Sagripanti. 2006. Environmental Factors that Effect The Survival and Persistance of Burkholderia pseudomallei American Society for Microbiol. 72 (11): 6865-6875. http//aem.asm.org.06/08/2008.
Irianto, A. 2003. Probiotik Akuakultur. Gadjah Mada University Press. 125 hal.
Madigan, M.T., V.M. Martinko, and J. Parker. 2003. Biologi of Microorganism. Eighth Edition. Prentince Hall, Inc., New Jersey.
Prihartono, K, Rasidik, J dan Arie, U. 2000. Mengatasi Permasalahan Budidaya Lele Dumbo. Penebar Swadaya. Jakarta.
Roberts M., M. E. Mercade, M. P. Bosch, J. L. Parra, M. J. Espuny, M. A. Manresa dan J. Guinea. 1989. Effect of Carbon Source on Biosurfactant Production by Pseudomonas aeruginosa 44T1. Biotech Lett. 11:871-874.
Saanin, H. 1984. Taksonomi dan Kunci Identifikasi Ikan vol I dan II. Bina Cipta Press. Jakarta.
Salle, A. J. 1991. Fundamental Principles of Bacteriology Fifth Edition. Mc Graw Hill Book Company. Ney York. 812 p.
Sasongko, A. 2001. Biomassa Bakteri Nitrifikasi pada Berbagai Bahan Filter dalam Sistem Resirkulasi Aliran Tertutup dan Pengaruhnya terhadap Kondisi Ikan. Program Studi Ilmu Perairan. IPB. 59 hal.
Sexena, R. K., P. K. Ghost, R. Gupta, W. S. Davidson, S. Bradoo dan R. Gulati. 2004. Microbial Lipase: Potensial Biocatalysts for Tha Future Industry. http:/www.las.ac.in/currsci/jul10/articles18.htl. Akses 13/10/09.
Suarsini, E. 2006. Bioremediasi Limbah Cair Rumah Tangga menggunakan Konsorsia Bakteri Indigen yang berpotensi Pereduksi Polutan. Disertasi Program Studi Pendidikan Biologi. Universitas Negeri Malang. 211 hal.