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Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

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Fish Health Management

The Effect of Immersion Duration of Zoothamnium penaei Crude Protein Extracts to Stimulate Immune System in Litopenaeus vannamei Against Enterocytozoon hepatopenaei (EHP)

https://doi.org/10.20473/jipk.v15i2.38349
Anord Charles Nkuba
Aqua-farms Organization, Sinza C, Dar es Salaam, 22564. Tanzania
https://orcid.org/0000-0003-1592-7776
Gunanti Mahasri
Department of Fish Health Management and Aquaculture, Universitas Airlangga, Surabaya, East Java, 60115. Indonesia
https://orcid.org/0000-0003-2016-9076
Nunuk Dyah Retno Lastuti
Department of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115. Indonesia
https://orcid.org/0000-0002-2832-4838
Adamu Ayubu Mwendolwa
Department of Biological Sciences, Mkwawa University College of Education, Iringa, 2513. Tanzania
https://orcid.org/0000-0002-9433-767X

Corresponding Author(s) : Gunanti Mahasri

mahasritot@gmail.com

Jurnal Ilmiah Perikanan dan Kelautan, Vol. 15 No. 2 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN

Abstract

Highlight Research



  1. The effect of immersion duration of shrimp to the immunostimulant from crude protein Zoothamnium penaei was determined.

  2. 20-minute immersion duration in crude protein from Zoothamnium penaei was found to increase the phagocytosis activities, phenoloxidase, and improve hepatopancreases of the shrimp.

  3. There is increase in phagocytosis activities, phenoloxidase and improved hepatopancreases cell within a three-week maintenance of shrimp with immersion after each seven days.

  4. There is interaction between immersion duration and maintenance time of shrimp into the crude protein from Zoothamnium penaei.


Abstract


Enterocytozoon hepatopenaei (EHP) is a spore-forming microsporidia intracellular parasite, which slough off the cells of the hepatopancreases of the shrimp. This study was intended to determine the effect of immersion duration and maintenance time to stimulate the immune system of shrimps by using a crude protein from Zoothamnium penaei to protect against EHP infections. The study used a completely randomized design where shrimps were divided into four groups each having five replicates. Groups P1, P2, P3 were immersed in immunostimulant for 10, 15, and 20 minutes respectively, except negative control. The phenoloxidase, phagocytosis activities, histopathology study were determined. Also, the molecular diagnosis of EHP was measured to confirm EHP infection in the shrimp samplesThe phenoloxidase activity significantly increased after immersion for 20 minutes (P3) from 0.64-, 0.72-, and 0.78-units min-1 mil -1 of protein on the first week, second week and third week, respectively. The phagocytosis activities of the sample immersed for 20 minutes were significantly increased to 68% on the second week. The sample also showed improved hepatopancreases epithelial cells with only 26% necrotic cell, less EHP spore and vacuolation. There was an interaction between immersion duration and maintenance time of the L. vannamei immersed in crude protein from Zoothamnium penaei. Twenty minutes immersion were significantly found to stimulate the immune system of the shrimp against EHP. The present work revealed that, the application of crude protein from Z. penaei is effective against EHP in shrimp culture.

Keywords

shrimp phagocytosis phenoloxidase Microsporidia Hepatopancreas
Nkuba, A. C., Mahasri, G. ., Lastuti, N. D. R., & Mwendolwa, A. A. . (2023). The Effect of Immersion Duration of <I>Zoothamnium penaei</I> Crude Protein Extracts to Stimulate Immune System in <I>Litopenaeus vannamei</I> Against <I>Enterocytozoon hepatopenaei</I> (EHP). Jurnal Ilmiah Perikanan Dan Kelautan, 15(2), 408–419. https://doi.org/10.20473/jipk.v15i2.38349
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Author Biographies

Anord Charles Nkuba, Aqua-farms Organization, Sinza C, Dar es Salaam, 22564. Tanzania

Study Program Fisheries and Marine Biotechnology, Faculty of Fisheries and Marine, Universitas Airlangga, Surabaya, East Java, 60115. Indonesia

Aqua-farms Organization, Sinza C, Dar es Salaam, 22564. Tanzania

Gunanti Mahasri, Department of Fish Health Management and Aquaculture, Universitas Airlangga, Surabaya, East Java, 60115. Indonesia

Department of Fish Health Management and Aquaculture, Universitas Airlangga, Surabaya, East Java, 60115. Indonesia

Nunuk Dyah Retno Lastuti, Department of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115. Indonesia

Department of Parasitology, Faculty of Veterinary Medicine, Universitas Airlangga, Surabaya, East Java, 60115. Indonesia

Adamu Ayubu Mwendolwa, Department of Biological Sciences, Mkwawa University College of Education, Iringa, 2513. Tanzania

Department of Biological Sciences, Mkwawa University College of Education, Iringa, 2513. Tanzania

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