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Effect of Ultrasonic Assisted Extraction with Ethanol for Removing Lipid on Catfish (Pangasius sp.) Skin as a Collagen Source and Its Characteristics
Corresponding Author(s) : Patmawati Patmawati
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 16 No. 1 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
Fish skin is rich in collagen (80%-90%). However, catfish (Pangasius sp.) skin has a high lipid content, which can reduce the quality of collagen. Therefore, treatment is needed to remove the lipid using ultrasonic assisted extraction (UAE) with ethanol. Hence, the aim of the present study was to remove lipid content from catfish skin as a raw material for collagen by using UAE with different concentrations of ethanol (25, 50, and 75%). The research was conducted in three stages: removing impurities, collagen extraction, and characterization of collagen. The result exhibited that UAE-ethanol treatment was capable of removing up to 85.6% of lipid content, and the produced collagen had the potential to be utilized as an alternative source of collagen based on its properties.
Highlight Research
- Ethanol could be used as extractor of lipid from catfish skin of Pangasius
- Ultrasonic assisted extraction could increase efficiency of lipid extraction from catfish skin.
- Fish skin of Pangasius sp. has a potential source for collagen.
- Collagen from skin of Pangasius sp. has a good characteristic with high protein content.
Keywords
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- Agarwal, C., Máthé, K., Hofmann, T., & Csóka, L. (2018). Ultrasound-assisted extraction of cannabinoids from Cannabis Sativa L. Optimized by response surface methodology. J.ournal of Food Science, 83(3):700–710.
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References
Abbas, M., Sharma, G., Dambire, C., Marquez, J., Alonso-Blanco, C., Proaño, K., & Holdsworth, M. J. (2022). An oxygen-sensing mechanism for angiosperm adaptation to altitude. Nature, 606:565-569.
Agarwal, C., Máthé, K., Hofmann, T., & Csóka, L. (2018). Ultrasound-assisted extraction of cannabinoids from Cannabis Sativa L. Optimized by response surface methodology. J.ournal of Food Science, 83(3):700–710.
Aminudin, A., Muhammad, S., Hamdan, R. H., Shaari, R., Nordin, M. F. M., Saufi, R. A., & Mei, S. J. (2015). Characterization of collagen extract from the skins of commercial freshwater fish. Jurnal Teknologi, 77(33).
Arumugam, G. K. S., Sharma, D., Balakrishnan, R. M., & Ettiyappan, J. B. P. (2018). Extraction, optimization and characterization of collagen from sole fish skin. Sustainable Chemistry and Pharmacy, 9:19–26.
BSN (Badan Standardisasi Nasional). (2020). Kolagen kasar dari sisik ikan-syarat mutu dan pengolahan. Jakarta: BSN RI.
Belwal, T., Ezzat, S. M., Rastrelli, L., Bhatt, I. D., Daglia, M., Baldi, A., Devkota, H. P., Orhan, I. E., Patra, J. K., Das, G., Anandharamakrishnan, C., Gomez-Gomez, L., Nabavi, S. F., Nabavi, S. M., & Atanasov, A. G. (2018). A critical analysis of extraction techniques used for botanicals: Trends, priorities, industrial uses and optimization strategies. TrAC Trends in Analytical Chemistry, 100:82–102.
Chan, S. S., Roth, B., Jessen F., Jakobsen, A. N., & Lerfall, J. (2021). Water holding properties of Atlantic salmon. Comprehensive Review in Food Science and Food Safety, 21(1):477-498.
Chemat, F., Rombaut, N., Sicaire, A. G., Meullemiestre, A., Fabiano-Tixier, A. S., & Albert-Vian, M. (2017). Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications: A review. Ultrasonics Sonochemistry, 34(1):540-560.
Chen, S., Chen, H., Xie, Q., Hong, B., Chen, J., Hua, F., Bai, K., He, J., Yi, R., & Wu, H. (2016). Rapid isolation of high purity pepsin-soluble type I collagen from scales of red drum fish (Sciaenops ocellatus). Food Hydrocolloids, 52:468–477.
Desmelati, Sumarto, Dewita, Dahlia, Syafrijal, & Sari, P. A. (2020). Determination of nano-collagen quality from sea cucumber Holothuria scabra. IOP Conference Series: Earth and Environmental Science, 430(1):012005.
Espinales, C., Romero-Peña, M., Calderón, G., Vergara, K., Cáceres, P. J., & Castillo, P. (2023). Collagen, protein hydrolysates and chitin from by-products of fish and shellfish: An overview. Heliyon, 9(e14937):1-12.
Fabella, N., Herpandi, H., & Widiastuti, I. (2018). Pengaruh Metode ekstraksi terhadap karakteristik kolagen dari kulit Ikan Patin (Pangasius pangasius). Jurnal FishtecH, 7(1):69-75.
Gadi, D. S., Trilaksani, W., & Nurhayati, T. (2017). The histological, extraction and characterization collagens yellow-pike conger Muarenesox talabon. Jurnal Ilmu dan Kelautan Tropis, 9(2)665-683.
Ghomi, E. R., Nourbakhsh, N., Kenari, M. A., Zare, M., & Ramakrishna, S. (2021). Collagen-based biomaterials for biomedical applications. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 109(12):1986–1999.
Girsang, V., Reveny, J., & Nainggolan, M. (2020). Isolation and characterization collagen of patin skin (Pangasius sp.). Asian Journal of Pharmaceutical Research and Development, 8(1):47-51.
Gómez-Guillén, M. C., Giménez, B., López-Caballero, M. E., & Montero, M. P. (2011). Functional and bioactive properties of collagen and gelatin from alternative sources: A Review. Food Hydrocolloids, 25(8):1813-1827.
Guimarí£es, C. F. M., Mársico, E. T., Monteiro, M. L. G., Lemos, M., Mano, S. B., & Junior, C. A. C. (2016). The chemical quality of Viatnemese Pangasius hypopthalmus fillets. Food Science and Nutrition, 4(3):398-408.
Gurtovenko, A. A., & Anwar, J. (2009). Interaction of ethanol with biological membranes: the formation of non-bilayer structures within the membrane interior and their significance. The Journal of Physical Chemistry B, 113(7):1983-1992.
Hadinoto, S., Kolanus, J. P. M., & Idrus, S. (2019). Karakterisasi gelembung renang ikan tuna sirip kuning (Thunnus sp.) dan kolagen yang dihasilkan melalui ekstraksi asam asetat. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 14(2):129-140.
Hastarini, E., Fardiaz, D., Irianto, H. E., & Budijanto, S. (2012). Characteristics of fish oil produced from fillet processing waste of Siam (Pangasius hypopthalmus) and Jambal (Pangasius djambal) catfish. Agritech, 32(4):403-410.
Jaeschke, D. P., Menegol, T., Rech, R., Mercali, G. D., & Marczak, L. D. F. (2016). Carotenoid and lipid extraction from Heterochlorella luteoviridis using moderate electric field and ethanol. Process Biochemistry, 51(10):1636-1643.
Jin, H. X., Xu, H. P., Li, Y., Zhang, Q. W., & Xie, H. (2019). Preparation and evaluation of peptides with potential antioxidant activity by microwave assisted enzymatic hydrolysis of collagen from sea cucumber Acaudina molpadioides obtained from Zhejiang Province in China. Marine Drugs, 17(3):169.
Kadam, H. K., & Tilve, S. G. (2015). Advancement in methodologies for reduction of nitroarenes. RSC Advance, 83391-84307.
Karim, A., Islam, M. A, Khalid, Z. B., Faizal, C. K. M., Khan, M. M. R., & Yousuf, A. (2020). Microalgal cell disruption and lipid extraction techniques for potential biofuel production. In A. Yousuf (Ed.), Microalgal cultivation for biofuels production. (pp. 129-147). Cambridge: Academic Press.
Kartika, I. W. D., Trilaksani, W., & Adnyane, I. K. M. (2016). Characterization of collagen from swim bladder waste of yellow-pike (Muraenesox talabon) by acid and hydrothermal extraction. Jurnal Pengolahan Hasil Perikanan Indonesia, 19(3):222-232.
Khan, M. L., Vian, M. A., Fabiano-Tixier, A. S., Dangles, O., & Chemat, F. (2010). Ultrasound- assisted extraction of polyphenols (flavanone glycosides) from orange (Citrus sinensis L.) peel. Food Chemistry, 119(2):851–858.
Kim, H. K., Kim, Y. H., Kim, Y. J., Park, H. J., & Lee, N. H. (2012). Effects of ultrasonic treatment on collagen extraction from skins of the sea bass Lateolabrax japonicus. Fisheries Science, 78(2):485-490.
Li, Z. R., Wang, B., Chi, C. F., Zhang, Q. H., Gong, Y. D., Tang, J. J., Luo, H. Y., & Ding, G. F. (2013). Isolation and characterization of acid soluble collagens and pepsin soluble collagens from the skin and bone of Spanish mackerel (Scomberomorous niphonius). Food Hydrocolloids, 31(1):103-113.
Lin, Y. J., Le, G. W., Wang, J. Y., Li, Y. X., Shi, Y. H., & Sun, J. (2010). Antioxidative peptides derived from enzyme hydrolysis of bone collagen after microwave assisted acid pre-treatment and nitrogen protection. International Journals of Molecular Sciences, 11(11):4297–4308.
Lu, W. C., Chiu, C. S., Chan, Y. J., Mulio, A. T., & Li, P. H. (2023). Characterization and biological properties of marine by-product collagen through ultrasound-assisted extraction. Aquaculture Reports, 29(101514):1-15.
Normah, I., & Afiqah, M. M. (2018). Effect of extraction time on the physico-chemical characteristics of collagen from sin croaker (Johniecop sina) waste. International Food Research Journal, 25(3):1074-1080.
Nowsad, A. K. M. (2007). Participatory training of trainers: a new approach applied in fish processing. Bangladesh: Bangladesh Fisheries Research Forum.
Nurilmala, M., Suryamarevita, H., Hizbullah, H. H., Jacoeb, A. M., & Ochiai, Y. (2022). Fish skin as biomaterial for halal collagen and gelatin. Saudi Journal of Biological Sciences, 29(2):1100-1110.
Matinong, A. M. E., Chisti, Y., Pickering, K. L., & Haverkamp, R. G. (2022). Collagen extraction from animal skin. Biology, 11(6):905.
Muyonga, J. H., Cole, C. G. B., & Duodu, K. G. (2004). Characterization of acid soluble collagen from skins of young and adult Nile perch (Lates niloticus). Food Chemistry, 85(1):81–89.
Pal, G. K., & Suresh, P. V. (2016). Sustainable valorisation of seafood by-products: Recovery of collagen and development of collagen-based novel functional food ingredients. Innovative Food Science & Emerging Technologies, 37(Part B):201–215.
Patra, M., Salonen, E., Terama, E., Vattulainen, I., Faller, R., Lee, B. W., Holopainen, J., & Karttunen, M. (2006). Under the influence of alcohol: the effect of ethanol and methanol on lipid bilayers. Biophysical Journal, 90(4):1121-1135.
Pingret, D., Fabiano-Tixier., A. S. F., & Chemat, F. (2013). Ultrasound-assisted extraction, natural product extraction. Food Engineering Review, 89–1112.
Pezeshk, S., Rezaei, M., & Abdollahi, M. (2022). Impact of ultrasound on extractability of native collagen from tuna by-product and its ultrastructure and physicochemical attributes. Ultrasonics Sonochemistry, 89:106129.
Rao, J. V., Pallela, R., & Prakash, G. V. S. B. (2012). Prospect of marine sponge collagen and its application in cosmetology. In S. K. Kim (Ed.), Marine cosmeceuticals trends and prospects. (pp. 77-103). Boca Raton: CRC Press.
See, S. F., Hong, P. K., Wan Aida, W. M., & Babji, A. S. (2010). Physicochemical of gelatins extracted from skin of different freshwater fish species. International Food Research Journal, 17:809-816.
Schmidt, M. M., Dornelles, R. C. P., Mello, R. O., Kubota, E. H., Mazutti, M. A., Kempka, A. P., & Demiate, I. M. (2016). Collagen extraction process. International Food Research Journal, 23(3):913-922.
Senadheera, T. R. L., Dave, D., & Shahidi, F. (2020). Sea cucumber derived type I collagen: A comprehensive review. Marine Drugs, 18(9):471.
Shaik, M. I., Chong, J. Y., & Sarbon, N. M. (2021). Effect of ultrasound-assisted extraction on the extractability and physicochemical properties of acid and pepsin soluble collagen derived from Sharpnose stingray (Dasyatis zugei) skin. Biocatalysis and Agricultural Biotechnology, 38:102218.
Shirsath, S., Sonawane, S., & Gogate, P. (2012). Intensification of extraction of natural products using ultrasonic irradiations - A review of current status. Chemical Engineering and Processing: Process Intensification, 53:10–23.
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