Efektivitas Vaksinasi Crude dan Soluble Protein Spora Myxobolus Koi terhadap Tingkat Kerusakan Usus Ikan Koi (Cyprinus carpio Koi)
[ The Effectivety Crude and Soluble Protein of Myxobolus Koi Spore againts Intestine Different Degrees in Koi (Cyprinus carpio Koi)]

Gunanti Mahasri, Rachma Woro Anggarani, Lucia Tri Suwanti

= http://dx.doi.org/10.20473/jipk.v7i1.11233
Abstract views = 311 times | downloads = 544 times

Abstract


Abstract
Myxobolus koi is one species of Myxobolus sp that can cause parasitic diseases in fish called Myxobolusis. Based on the Decree of the Minister of Marine and Fisheries No: KEP.03/MEN/2010 that Myxobolus koi in the list of Fish Quarantine Pests group I. Myxosporean diseases are most numerous in the water can cause Proliferative Kidney Disease (PKD) and Whirling Disease (WD). The aim of this research is to finding, analyzing and determining the protein of spores Myxobolus koi that can effectively reduce the level of damage to the intestinal organs as well as for the prevention myxobolusis on Koi’s. Then for finding, analyzing and determining the protein of spores Myxobolus koi do isolation of spore proteins. The study consisted of three phases examination to preparation and identification of spores, isolation and analyze of crude and soluble protein spores for obtain dose and molecular weight each protein and histopathological test. This research uses descriptive method. The data presented may be narratives, images, tables or charts for each group. Intestinal histopathology test results of research carried scoring Koi’s were analyzed using the Kruskal-Wallis. The results showed a profile crude protein and soluble proteins from spores Myxobolus koi showed that the molecular weight of crude protein Myxobolus koi in this study was 150 kDa and 72 kDa and for soluble protein was 73 kDa. Results scoring the degree of infection caused by exposure to Myxobolus koi then statistically processed with an average yield of scoring in a sequence of 0; 1.6; 0.64 and 0.32. Statistical analysis showed no significant difference in the treatment of K + with crude protein, and K + with soluble proteins. Statistical analysis showed that there were significant differences in treatment with K+ and K-, K- with soluble protein and crude protein and soluble protein. Histopathological changes in the intestine in the form of inflammatory cell infiltration, necrosis and haemorage


Keywords


Myxobolus, crude protein, soluble protein, histopathology, intestines, koi fis

Full Text:

PDF

References


Chavda, D., S. Bhatt., R.A. Sreepada and A. Sheth. 2010. Pathogenicity of Myxobolus koi Infection and its Effect on Protein Expression in Catla catla in Central Gujarat Region. Journal of Cell and Tissue Research Vol 10(1): 21572164.

Delashoub, M., I. Pousty and S. M. B. Khojasteh. 2010. Histology of Bighead Carp (Hypophthalmichthys nobilis) Intestine. J. Global Vet. 5(6):302-306.

Fajriah, A. Karakterisasi Protein Lernea sp. Dengan menggunakan metode SDSPAGE. Surabaya. 2009. Hal. 38-40.

Klinger, R.E and R.F. Floyd. 2002. Introduction to Freshwater Fish Parasites. Institute of Food and Agricultural Sciences. University of Florida. Florida. 13p.

Lom, J and I. Dykova. 1995. Myxozoan Genera: Definition and Notes on Taxonomy, Life Cycle, Terminology and Pathogenic Species. J. Folia Parasitologica 53:1-36.

Mahasri, G. 2004. Ilmu Penyakit Ikan Protozoa pad Ikan dan Udang. Fakultas Perikanan dan Kelautan Universitas Airlangga. Surabaya. Hal 25-29.

Mahasri, G. 2007. Protein Membran Imunogenik Zoothamnium penaei Sebagai Bahan Pengembangan Imunostimulan pada Udang Windu terhadap Zoothamniosis. Disertasi Program Pascasarjana. Universitas Airlangga. Hal 284.

Mahasri, G. dan Kismiyati. 2011. Buku Ajar Parasit dan Penyakit Ikan I (Ilmu Penyakit Protozoa dan Udang). Universitas Airlangga, Surabaya. Hal 3-4.

Noga, E. J. 2010. Fish Disease: Diagnosis and Treatment. Wiley-Blackwell; Second edition. p.242-243.

Plumb J. A. 1994. Health Maintenance and principal Microbial Diseases of Cultured Fishes. Iowa State University CRC Press, Boca Raton, Florida. p.144.

Plumb, J. A. and Hanson, L. A. 2011. Health Maintenance and Principal Microbial Disease of Cultured Fishes. 3th Edition. Blackwell. Singapore. p. 39-45.

Ratnasari., R. H.E. Narumi, dan Wijayanto.,S. 2005. Isolasi dan Pengukuran Berat Molekul Protein Outer Membrane Protein (OMP) Kuman Salmonella pullorum Isolat Lapangan. Skripsi. Fakultas Kedokteran Hewan. Universitas Airlangga. Hal 28.

Rukyani, A. 1990. Histopathological Changes in The Gill of Common Carp (Cyprinus carpio L.) Infected with the Myxosporean Parasite MyxobolusKoi Kudo, 1920. Asian Fisheries Science (3):337-341.

Satyantini, W. H., E. D. Masithah, M. A. Alamsjah, Prayogo dan S. Andriyono. 2012. Penuntun Praktikum Budidaya Pakan Alami. Universitas Airlangga. Surabaya. hal. 49.

Schaperlaus, W. 1992. Fish Disease. Vol.2. Schaperlaus, W, H. Kulow, K., Schereckenbach, K. (Eds.), A.A. Balkema. Rotterdam. p.245.

Takashima, F. And T. Hibiya. 1995. An Atlas of Fish Histologi: Normal and Pathological Features. 2ndEdition. Kodansha. Tokyo. p.67-68, pp.88-90


Refbacks

  • There are currently no refbacks.


Copyright (c) 2015 Jurnal Ilmiah Perikanan dan Kelautan

 JIPK IS INDEXED BY :

                  

       

     

                      

           

 

             

          

     

                  

      

      

       

     

     

       

                     

View JIPK Stats

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License

 

EDITORIAL  OFFICE

JURNAL ILMIAH PERIKANAN DAN KELAUTAN (JIPK) /SCIENTIFIC JOURNAL OF FISHERIES AND MARINE

FAKULTAS PERIKANAN DAN KELAUTAN, UNIVERSITAS AIRLANGGA
Kampus C UNAIR, Jl. Dharmahusada Permai No.330, Mulyorejo,
Kota Surabaya, Provinsi Jawa Timur. Indonesia. 60115
Telepon: (031) 5911451
Fax. (031) 5965741
Email : jipk@fpk.unair.ac.id

 

 

 This Journal is Supported by