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Identifikasi Koi Herpesvirus pada Dosis yang Berbeda dengan Metode Imunohistokimia Streptavidin Biotin pada Ikan Nila (Oreochromis niloticus)
[Identification of Koi Herpes Virus At Different Dose with Streptavidin Biotin Methods Immunohistochemistry on Tilapia (Oreochromis niloticus)]
Corresponding Author(s) : Hari Suprapto
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 6 No. 2 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
KHV is a viral disease in goldfish and Koi Fish (Cyprinus carpio) is a highly contagious, infecting all ages or sizes of fish and aquaculture systems. This disease results in mortality between 80% - 100% of the total fish population, with an incubation period of between 1-7 days. KHV infection triggered by a drop in ambient temperature so it is referred to as a virus that attacks when cold (a cold virus). The disease attacks the temperature range of 18-28 ° C and can cause death. This virus attacks can occur at any age of the fish ranging in size from the seed to the parent. The most prominent clinical symptoms due to KHV infection is the sudden death 1-2 days after infection. Other clinical symptoms are necrosis of the gills, sunken eyes, bleeding at the gills, hemorraghe, excess mucus production in the body, and secondary bacterial infections or parasitic infestations. Streptavidin biotin immunohistochemical method was applied to the study of cells and tissues by staining imunostaining. Technique of determining the existence of (location) antigen (target protein) in tissue using antigen-antibody reaction that begins with histotenik procedure is the procedure of making tissue sections (histology). This method has high sensitivity and fast so that it can be applied to the detection of KHV antigen in tissues of fish Tilapia (Orechromis niloticus). This study aims to determine the presence of KHV antigen in tilapia gills after infection. The method used in this study is the experimental method. Dose of virus titer used was 1 ID50, ID50 10, 100 ID50, ID50 1000. The results showed that the Streptavidin Biotin immunohistochemical examination imunopatologi able to detect KHV virus Ag in the gill tissue of tilapia (Oreochromis niloticus) were infected with different doses. In all treatments showed on all the gills are KHV antigen is indicated by the presence of a golden brown color on the gill epithelium. Advised Streptavidin biotin immunohistochemistry test was applied to the detection of the presence or absence of fish-carp KHV carrier as a routine control program and control, including the prevention and outbreak of KHV in Indonesia, because of KHV were attacked in tilapia was persistent and did not show clinical symptoms
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- Bercovier H, Fishman Y, Nahary R, Sinal S, Ziotkin A, Eyngor M, Gilad O, Eldar A, Hedrick RP 2005. Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis. BMC Microbiol 5: 13-19.
- Brandtzaeg, P. 1998. The Increasing Power Of Immunohistochemistry and immunocytochemistry. Journal of Immunology 216; 49-67.
- Daili SF dan. Makes WIB. 2002. Infeksi virus herpes. Jakarta : Balai Penerbit Fakultas Kedokteran UI.
- Damayanti, R., Alfinus, I., Rahmadiani., Faizal. 2009. Deteksi Antigen Virus Rabies Pada Jaringan Otak Dengan Metode Imunohistokimia. Seminar Nasional teknologi Petrnakan dan Veteriner; 707-719.
- El-Matbouli, M. and H. Soliman. 2011. Transmission of Cyprinid herpesvirus3 (CyHV-3) from goldfish to naȉ ve common carp by cohabitation. Research in Veterinary Science 90: 536-539.
- Fenner FJ, Gibbs EPJ, Murphy FA, Rott R, Studdert MJ and White DO. 1993. Virologi veteriner. California : Academic Press. Gilad O., Yun S., Zagmutt-Vergara FJ., Leutenegger CM., Bercovier H., Hendrick RP. 2004. Concentrations of a koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR. Dis Aqua Org 60: 179187.
- Gilad, O., Yun,S., adkidson. M.A., Way, K., Willits, N.H., bercovier, H., and Hedrick, R.P. 2003. Molecular Comparison of Isolate of An Emerging Fish Pathogen, Koi Herpesvirus, and The Effect of Water Temperature on Mortality of Experimentaly Infected Koi. I Gen Virol, 84 ; 2661-2668.
- Hartman KH, Yanong RPE, Petty BD, Floyd RF and Riggs AC. 2004. Koi herpes virus (KHV) disease. Publikasi internet.. http://www.edis.ifasSedu.
- Isnansetyo, A. dan Kurniastuti. 1995. Teknik Kultur Phytoplankton dan Zooplankton Pakan Alami untuk Pembenihan Organisme Laut. Penerbit Kanisius. Yogyakarta. Hal 60- 63.
- Kusriningrum. 2008. Perancangan Percobaan. Airlangga University Press. Surabaya. hal 21.
- Lio-Po, G., et. al. 2009. Surveillance of Emerging Fish Viral Pathogens in Some Southeast Asian Countries. The Israeli Journal of AquacultureBamidgeh 61(3): 208-214.
- Magnadottir B. 2006. Innate immunity of fish (overview). Fish and shellfish Immunology 20 : 137-1 5 1.
- Nat DR. 2001. Characterization of immune system of carp (Cyprinus carpio L.) to the hemoflagellata Trypanoplasma borelly Laveran and Mesnil. Disertation. Der Universitat Hannover.
- Saphira, Y., et al. 2005. Differential Resistance to KOi herpesvirus (KHV) Carp Interstitial Nephritis and Gill Necrosis Virus (CNGV) Among Common Carp (Cyprinus carpio) Strain and Croos Breds. Aquaculture 245; 11.
- Siregar, S. 2011. Statistika Deskriptif untuk Penelitian. Dilengkapi Perhitungan Normal & Aplikasi SPSS versi 17. PT. Raja Grafindo Persada. Jakarta. hal 324.
- Sunarto, A., et. al. 2005b. Field Investigations on a Serious Disease Outbreak among Koi and Common Carp (Cyprinus carpio) in Indonesia. Diseases in Asian Aquaculture V. hal. 125-135.
- Sunarto, A., et al. 2011. Isolation and Characterisation of Koi herpesvirus (KHV) from Indonesia Identification of a New Lineage. Jurnal of Fish Disease. 34; 87-101.
- Suprapto, H. 2011. Histologi Ikan. Fakultas Perikanan dan Kelautan Universitas Airlangga. Surabaya. hal 6-13.
- Taukhid, A. M. Lusiastuti, W. Andiyani, Rosidah, dan Sriati. 2010. Induksi Kekebalan Spesifik pada Ikan Mas, Cyprinus carpio Linn. Terhadap Infeksi Koi herpevirus (KHV) melalui Teknik Kohabitasi Terkontrol. Balai Riset Budidaya Air Tawar. 257-276.
- Taukhid, A., Sunarto, A., Koesharyani, I., Supriyadi, H., Gardenia. Strategi Pengendalian Penyakit Koi Herpesvirus (KHV). 2004. Balai Riset Budidaya Air Tawar.
- Wasito, R., Hastari, W., Bambang, S. 2013a. Identifikasi KHV Dengan Uji Imunopatologi Imunohistokimia Streptavidin Biotin Pada Ikan Mas Karier. Jurnal Veteriner 14. No. 1: 3744.
References
Bercovier H, Fishman Y, Nahary R, Sinal S, Ziotkin A, Eyngor M, Gilad O, Eldar A, Hedrick RP 2005. Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis. BMC Microbiol 5: 13-19.
Brandtzaeg, P. 1998. The Increasing Power Of Immunohistochemistry and immunocytochemistry. Journal of Immunology 216; 49-67.
Daili SF dan. Makes WIB. 2002. Infeksi virus herpes. Jakarta : Balai Penerbit Fakultas Kedokteran UI.
Damayanti, R., Alfinus, I., Rahmadiani., Faizal. 2009. Deteksi Antigen Virus Rabies Pada Jaringan Otak Dengan Metode Imunohistokimia. Seminar Nasional teknologi Petrnakan dan Veteriner; 707-719.
El-Matbouli, M. and H. Soliman. 2011. Transmission of Cyprinid herpesvirus3 (CyHV-3) from goldfish to naȉ ve common carp by cohabitation. Research in Veterinary Science 90: 536-539.
Fenner FJ, Gibbs EPJ, Murphy FA, Rott R, Studdert MJ and White DO. 1993. Virologi veteriner. California : Academic Press. Gilad O., Yun S., Zagmutt-Vergara FJ., Leutenegger CM., Bercovier H., Hendrick RP. 2004. Concentrations of a koi herpesvirus (KHV) in tissues of experimentally infected Cyprinus carpio koi as assessed by real-time TaqMan PCR. Dis Aqua Org 60: 179187.
Gilad, O., Yun,S., adkidson. M.A., Way, K., Willits, N.H., bercovier, H., and Hedrick, R.P. 2003. Molecular Comparison of Isolate of An Emerging Fish Pathogen, Koi Herpesvirus, and The Effect of Water Temperature on Mortality of Experimentaly Infected Koi. I Gen Virol, 84 ; 2661-2668.
Hartman KH, Yanong RPE, Petty BD, Floyd RF and Riggs AC. 2004. Koi herpes virus (KHV) disease. Publikasi internet.. http://www.edis.ifasSedu.
Isnansetyo, A. dan Kurniastuti. 1995. Teknik Kultur Phytoplankton dan Zooplankton Pakan Alami untuk Pembenihan Organisme Laut. Penerbit Kanisius. Yogyakarta. Hal 60- 63.
Kusriningrum. 2008. Perancangan Percobaan. Airlangga University Press. Surabaya. hal 21.
Lio-Po, G., et. al. 2009. Surveillance of Emerging Fish Viral Pathogens in Some Southeast Asian Countries. The Israeli Journal of AquacultureBamidgeh 61(3): 208-214.
Magnadottir B. 2006. Innate immunity of fish (overview). Fish and shellfish Immunology 20 : 137-1 5 1.
Nat DR. 2001. Characterization of immune system of carp (Cyprinus carpio L.) to the hemoflagellata Trypanoplasma borelly Laveran and Mesnil. Disertation. Der Universitat Hannover.
Saphira, Y., et al. 2005. Differential Resistance to KOi herpesvirus (KHV) Carp Interstitial Nephritis and Gill Necrosis Virus (CNGV) Among Common Carp (Cyprinus carpio) Strain and Croos Breds. Aquaculture 245; 11.
Siregar, S. 2011. Statistika Deskriptif untuk Penelitian. Dilengkapi Perhitungan Normal & Aplikasi SPSS versi 17. PT. Raja Grafindo Persada. Jakarta. hal 324.
Sunarto, A., et. al. 2005b. Field Investigations on a Serious Disease Outbreak among Koi and Common Carp (Cyprinus carpio) in Indonesia. Diseases in Asian Aquaculture V. hal. 125-135.
Sunarto, A., et al. 2011. Isolation and Characterisation of Koi herpesvirus (KHV) from Indonesia Identification of a New Lineage. Jurnal of Fish Disease. 34; 87-101.
Suprapto, H. 2011. Histologi Ikan. Fakultas Perikanan dan Kelautan Universitas Airlangga. Surabaya. hal 6-13.
Taukhid, A. M. Lusiastuti, W. Andiyani, Rosidah, dan Sriati. 2010. Induksi Kekebalan Spesifik pada Ikan Mas, Cyprinus carpio Linn. Terhadap Infeksi Koi herpevirus (KHV) melalui Teknik Kohabitasi Terkontrol. Balai Riset Budidaya Air Tawar. 257-276.
Taukhid, A., Sunarto, A., Koesharyani, I., Supriyadi, H., Gardenia. Strategi Pengendalian Penyakit Koi Herpesvirus (KHV). 2004. Balai Riset Budidaya Air Tawar.
Wasito, R., Hastari, W., Bambang, S. 2013a. Identifikasi KHV Dengan Uji Imunopatologi Imunohistokimia Streptavidin Biotin Pada Ikan Mas Karier. Jurnal Veteriner 14. No. 1: 3744.