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Efektifitas Ekstrak Tanaman Meniran (Phyllanthus niruri) Sebagai Antibakteri Edwardsiella tarda Secara In Vitro
[Effectivity Of Meniran (Phyllanthus niruri) Extract as Edwardsiella tarda Antibacterial According In Vitro]
Corresponding Author(s) : Sudarno Sudarno
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
Medicinal plants proven to effectively cope with bacterial diseases one of which is the plant meniran (Phyllanthus niruri). Phyllanthus niruri can act as an antibacterial because it has an antibacterial ingredient content of flavonoids, alkaloids, phenols, and tannins. This shows that plants can be used as an antibacterial Phyllanthus niruri of E.tarda. The purpose of this study was to determine the ability of the antibacterial of Phyllanthus niruri extracts and to find out the best concentration of Phyllanthus niruri extracts as antibacterial E.tarda according in vitro. The experiment was conducted in January 2011, housed at the Laboratory of Bacteriology Juanda Fish Quarantine Center and Laboratory of Organic Chemistry and Technology Faculty of Airlangga University Saints. This research method is done by the Minimum Inhibitor Concentration (MIC) and Minimum Bacterial Concentration (MBC) with 12 treatment. The results showed that the best concentration for the treatment of the concentration of 0.0313 g / ml as indicated with no growth of bacteria and is the lowest concentration of the dilution.
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- Afrianto, E dan E. Liviawaty. 1992. Pengendalian Hama dan Penyakit Ikan. Penerbit Kanisius. Yogyakarta.
- Ajizah, A. 2004. Sensitivitas Salmonella Typhimurium Terhadap Ekstrak Daun Psidium Guajava L. Bioscientiae, Vol. 1, No. 1 : 31-8.
- Berghe, V. 1991. Screening Methods For Bacterial Antiviral Agents For Higher Plants. Harcource Brace Jauvanovich Publ. London.
- Dwidjoseputro, D. 1994. Dasar-Dasar Mikrobiologi. Djambatan. Jakarta.
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- Ewing, H. W. and P. R. Edward. 1973. Identification Of Enterobactericeae By Biochemichal Reaction. Burgess Publishing Co. Mineapolis. 61-89 P.
- Health Agency. 2001. Edwardsiella tarda. Material Safety Data Sheets (MSDS). Public Health Agency of Canada. Jan.
- Heyne, K. 1987. Tanaman Berguna Indonesia, Jilid II. Yayasan Sauna Wana Jaya. Jakarta. Halaman 1138-114.
- Leotta, G., and V. Germán. 2009. Prevalence of E.tarda in Antarctic wildlife Polar Biology. Volume 35. 809-812 P.
- Masduki, I. 1996. Efek Antibakteri Ekstrak Biji Pinang (Areca catechu) terhadap S. aureusan Bakteri. Cermin Dunia Kedokteran 109. Halaman 214.
- Michael, J. P and E. C. S. Chan. 1988. Dasar-Dasar Mikrobiologi. Universitas Indanesia Press. Jakarta. Halaman 808-813.
- Rao, P. S., Y. Yamada., Y . P. Tan., and K. Y. Leung. 2004. Use of proteomics to identify novel virulence determinants that are required for Edwardsiella tarda pathogenesis. Mol Microbiol. 573-86 P
- Robinson, T. 1995. Senyawa Organik Tanaman Tingkat Tinggi. ITB. Bandung.
- Sidik dan A. Subarnas. 1993. Phyllanthus niruri L.; Kimia, Farmakologi dan Penggunaannya Dalam Obat Tradisional. Proseding Seminar Meniran dan Kedawung 13-14 Agustus 1993. Surabaya.
- Sudewo, B. 2007. Basmi Penyakit dengan Sirih Merah, PT Agromedia Pustaka, Jakarta.
- Tuti, D. S. 1997. Isolasi and Identifikasi Triterpinoid dari Batang Meniran. Skripsi. FF-UA. Surabaya.
- Wijayakusuma, H., S. Dalimartha., A. S. Wirian., B. Wibowo. 1996. Tanaman Berkhasiat Obat Di Indonesia. Edisi I. pustaka Kartini. Jakarta.Halaman 66-67.
References
Afrianto, E dan E. Liviawaty. 1992. Pengendalian Hama dan Penyakit Ikan. Penerbit Kanisius. Yogyakarta.
Ajizah, A. 2004. Sensitivitas Salmonella Typhimurium Terhadap Ekstrak Daun Psidium Guajava L. Bioscientiae, Vol. 1, No. 1 : 31-8.
Berghe, V. 1991. Screening Methods For Bacterial Antiviral Agents For Higher Plants. Harcource Brace Jauvanovich Publ. London.
Dwidjoseputro, D. 1994. Dasar-Dasar Mikrobiologi. Djambatan. Jakarta.
Ev a n s , W. C. 1 9 8 9 . Tr e a s e a n d Ev a n s Pharmacognosy Basic Of Therapeutics. 4th ed. W.B. Sanders. Bailliere Tindall. London. 420 P.
Ewing, H. W. and P. R. Edward. 1973. Identification Of Enterobactericeae By Biochemichal Reaction. Burgess Publishing Co. Mineapolis. 61-89 P.
Health Agency. 2001. Edwardsiella tarda. Material Safety Data Sheets (MSDS). Public Health Agency of Canada. Jan.
Heyne, K. 1987. Tanaman Berguna Indonesia, Jilid II. Yayasan Sauna Wana Jaya. Jakarta. Halaman 1138-114.
Leotta, G., and V. Germán. 2009. Prevalence of E.tarda in Antarctic wildlife Polar Biology. Volume 35. 809-812 P.
Masduki, I. 1996. Efek Antibakteri Ekstrak Biji Pinang (Areca catechu) terhadap S. aureusan Bakteri. Cermin Dunia Kedokteran 109. Halaman 214.
Michael, J. P and E. C. S. Chan. 1988. Dasar-Dasar Mikrobiologi. Universitas Indanesia Press. Jakarta. Halaman 808-813.
Rao, P. S., Y. Yamada., Y . P. Tan., and K. Y. Leung. 2004. Use of proteomics to identify novel virulence determinants that are required for Edwardsiella tarda pathogenesis. Mol Microbiol. 573-86 P
Robinson, T. 1995. Senyawa Organik Tanaman Tingkat Tinggi. ITB. Bandung.
Sidik dan A. Subarnas. 1993. Phyllanthus niruri L.; Kimia, Farmakologi dan Penggunaannya Dalam Obat Tradisional. Proseding Seminar Meniran dan Kedawung 13-14 Agustus 1993. Surabaya.
Sudewo, B. 2007. Basmi Penyakit dengan Sirih Merah, PT Agromedia Pustaka, Jakarta.
Tuti, D. S. 1997. Isolasi and Identifikasi Triterpinoid dari Batang Meniran. Skripsi. FF-UA. Surabaya.
Wijayakusuma, H., S. Dalimartha., A. S. Wirian., B. Wibowo. 1996. Tanaman Berkhasiat Obat Di Indonesia. Edisi I. pustaka Kartini. Jakarta.Halaman 66-67.