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Pengaruh Ekstrak Alga Cokelat (Sargassum sp.) terhadap Pertumbuhan Bakteri Escherichia coli Secara In Vitro
[Effect of Brown Algae Extract (Sargassum sp.) on Growth of Escherichia coli In Vitro]
Corresponding Author(s) : Moch. Amin Alamsjah
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 3 No. 1 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
Algae Sargassum sp. showed the ability to inhibit the growth of a maximum of several types of pathogenic bacteria such as Escherichia coli. Because of Sargassum sp. have antibacterial with 2 active ingredients are compounds of phenol and tannin fiber. So Sargassum sp. potential to serve as antibakteri substances against several types of bacterial pathogens such as bacteria that cause diarhea.The aim of the study was to determine antibacterial activity brown algae exstract (Sargassum sp.) and determine best concentration from brown algae exstract (Sargassum sp.) to kill of E. coli. This study was conducted at several places such as Saints and Technology Faculty and Veterinary Faculty, Airlangga University Surabaya. Research was done September 2010. This study was conducted in the laboratory using experimental method completely randomized design with twelve treatments and there replications. The main objectives of this study were MBC extract of Sargassum sp. Result of the study analyzed with Khi-Khuadrat. Result of the study showed that extract Sargassum sp. has antimicrobial activity against E. coli were determined with in vitro method. Based on MBC data were found to be effective to kill of E. coli at 50 %. Exploration potential of extracts from brown algae (Sargassum sp.) against various species of bacteria that cause diarrhea are expected to become a new information about the content of extracts of Sargassum sp. as an antibacterial. Also from this research will be obtained an alternative treatment of diarrheal diseases that come from living marine resources and will eventually increase the economic value of fishery commodities which have not explored its use as Sargassum sp.
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- Sprague Dawley. Fakultas Farmasi. Universitas Muhammadiyah Surakarta. Surakarta.
- Gonzales A., B. Angela., M. J. Rio and F. Pelaez. Screening of antimicrobial activities in red, green, and brown macroalgaes from Gran Canaria (Canary Islands, Spain). Spain. 4 : 35-40.
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- Purwanti, Elly. 2007. Senyawa Bioaktif Tanaman Sereh
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- Puspaningrum, Anglia. 2008. Penerapan Metode Konvensional pada Escherichia coli. Universitas Indonesia. Jakarta.
- Sastry and Rao. 1994. Antibacterial Substance From Marine Algae. Successive Extraction Using Benzene, Chloroform and Methanol. Department of Biochemistry, Institute of Medical Science, Banaras Hindu University. India.
- Schelfler, C. William. 1987. Statistika Untuk Biologi, farmasi, Kedokteran, dan Ilmu yang Bertautan. Terbitan kedua. ITB. Bandung.
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References
Acun, Sodiyc. 2010. Tabel Uji Biokimia Bakteri Familia Enterobacteriaceae. Jakarta. http://sodiycxacun.com/2010/07/tabel-ujibiokimia- bakteri-familia.html.
Ardian. 2010. Uji Biokimia Metabolisme Bakteri. Jakarta. http://dydear.multiply.com/journal/ item/3/Uji_Biokimia_Mikroba.
Bailey and Scott's. 1994. Diagnostic Microbiology. 8 th Edition. Toronto. p. 313-328. Bailey. W. R and E. G. Scott. 1986. Diagnostic Microbiology. 7 th Ed. The CV. Mosby Company. Saint Louis.
Christofilogiannis, P. 2001. Current Inoculation Me t h o d s i n MIC De t e rmi n a t i o n . Aquaculture, 208 : 1 – 10.
Cowan and Steel's. 1981. Manual for the Identification of Medical Bacteria. Second edition. Cambridge University Press. New York.
Depkes RI. 2000. Parameter Standart Umum Ekstrak Tumbuhan Obat. Direktorat Pengawasan Obat Tradisional. Jakarta.
Ernst, M. 1991. Dinamika Obat.Alih Bahasa : M. B. Widianto.ITB Press. Bandung.
Fanani, Rizal. 2009. Uji Toksisitas Akut Ekstrak Etanol Daun Dewandaru (Eugenia uniflora L. ) Per Oral pada Tikus Galur
Sprague Dawley. Fakultas Farmasi. Universitas Muhammadiyah Surakarta. Surakarta.
Gonzales A., B. Angela., M. J. Rio and F. Pelaez. Screening of antimicrobial activities in red, green, and brown macroalgaes from Gran Canaria (Canary Islands, Spain). Spain. 4 : 35-40.
Harbone, J. B. 1987. Metode Fitokimia. Penuntun Cara Menganalisa Tumbuhan. ITB.
Irwan. 2003. Menggali Manfaat Rumput Laut. Riset Unggulan ITB. Bandung.
Juliantina,F. R. 2007. Manfaat Sirih Merah (Piper crocatum) Sebagai Agen Anti Bakterial Gram Negatif dan Gram Positif. Jurnal Kedokteran dan Kesehatan Indonesia. Yogyakarta.
Kandhasamy, M dan K.D. Arunachalam. 2008. Evaluation of in vitro antibacterial property of seaweeds of southeast coast of India African Journal of Biotechnology Vol. 7 (12), pp. http://www.academicjournals.org/AJB. 18/09/2008.
Lennete, H. Edwin, E. H. Spaulding and J. P. Truant. 1974. Manual Clinical Microbiology. Second Edition. American society for Microbiology. Washington D.C.
Purwanti, Elly. 2007. Senyawa Bioaktif Tanaman Sereh
(Cymbopogon nardus) Ekstrak Kloroform dan Etanol serta Pengaruhnya Terhadap Mikroorganisme Penyebab Diare. Jurysan Pendidikan Biologi. Fakultas Pendidikan Biologi dan Ilmu Pendidikan. Universitas Muhammadiyah Malang.
Puspaningrum, Anglia. 2008. Penerapan Metode Konvensional pada Escherichia coli. Universitas Indonesia. Jakarta.
Sastry and Rao. 1994. Antibacterial Substance From Marine Algae. Successive Extraction Using Benzene, Chloroform and Methanol. Department of Biochemistry, Institute of Medical Science, Banaras Hindu University. India.
Schelfler, C. William. 1987. Statistika Untuk Biologi, farmasi, Kedokteran, dan Ilmu yang Bertautan. Terbitan kedua. ITB. Bandung.
Trono, J.R. G.C and E.T. Ganzon 1988. Philippine Seaweeds Publ. by National book store. Inc.