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Uji Potensi Sari Buah Belimbing Wuluh (Averrhoa bilimbi L.) dalam Menghambat Pertumbuhan Bakteri Aeromonas salmonicida Smithia Secara In Vitro
[Potential Test Cucumber Fruit Juice (Averrhoa bilimbi L.) In Inhibiting Growth Of Aeromonas salmonicida Smithia Bacteria By In Vitro]
Corresponding Author(s) : Prayogo Prayogo
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 3 No. 2 (2011): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
Giving antibiotics continuously can cause A. salmonicida smithia become resistant and residues of antibiotics may pollute the water environment. One alternative to using traditional medicinal plants that are antibacterial. Advantages of using traditional medicinal plants are relatively more secure, easily available, inexpensive, does not cause resistance, and relatively harmless to the surrounding environment. The content of natural chemicals from cucumber fruit is known to have antibacterial effects namely, flavonoids and phenols. The purpose of this study to determine ability the cucumber fruit juice inhibit bacterial growth A. salmonicida smithia and to determine the best concentration to inhibit bacterial growth A. salmonicida smithia with ampicillin drugs for comparison by in vitro. This research was conducted in the Bacteriology Laboratory of Fish Quarantine Center of Sidoarjo and Human Genetic Laboratory the Institute of Tropical Disease Centre, Airlangga University, Surabaya. This research use done eksperimental method by in vitro, with a large measure of cucumber fruit juice inhibition zone around the existing paper disc and comparing it to a large zone of inhibition using ampicillin. Concentration dilution series cucumber fruit juice used during the research, began 2 gr/ 2 ml to the concentration of 0,0625 gr/ml. Result of research show at concentration 0,125 gr/ml still has ability to pursue bacterium A. smithia salmonicida, so that can be anticipated that effective cucumber fruit juice if used as by drug, because at small concentration can to pursue bacteria A. smithia salmonicida by in vitro.
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References
Adriana, Y. 1992. Daya Hambat Sari Rimpang Kunyit (Curcuma domestica Val.) Terhadap Dua Jenis Jamur Dermatofita. Skripsi Fakultas Matematika dan Ilmu Pengetahuan Alam UNAND. Padang.
Adriani, A. 1992. Daya Antibakteri Allium sativum L. dari Pasar Bringharjo Yogyakarta Terhadap Staphylococcus aureus dan Escherichia coli Koleksi Laboratorium Mikrobiologi Fakultas Kedokteran Universitas Gajah Mada Secara In Vitro. Skripsi Fakultas Farmasi UGM. Yogyakarta.
Afrianto, E dan E. Liviawaty. 1992. Pengendalian Hama dan Penyakit Ikan. Kanisius. Yogyakarta. 89 hal.
Boyd, R. F. 1995. Basic Medical Microbiology. Fifth Edition. Little Brown and Company. USA.
Cipriano, R. C. and Bullock G. L. 2001. Furunculosis and Other Disease Caused By Aeromonas Salmonicida. Revision of "Furunculosis and other diseases caused by Aeromonas salmonicida,” by G. L. Bullock, R. C. Cipriano, and S. F. Snieszko, 1983. hal 9.
Departemen Kelautan dan Perikanan. 2007. Penyakit Ikan Karantina Golongan Bakteri. Pusat Karantina Ikan : Departemen Kelautan dan Perikanan. Jakarta. 5 hal.
Ernts, M. 1991. Dinamika Obat. Alih Bahasa : M. B. Widianto. ITB Press. Bandung. hal 30 – 40.
Harborner, J. B. 1987. Metode Fitokimia. Penuntun Cara Modern Menganalisa Tumbuhan. ITB Bandung. hal 71 – 77.
Hembing, W. 2008. Ramuan Lengkap Herbal Taklukkan Penyakit. Niaga Swadaya. Jakarta.
Holt, J. G., N. R. Krieg., P. H. A. Sneath., J. T. Staley and S. T. William. 1994. Bergey's Manual of Determinative Bacteriology. Ninth edition. William and Wilkins A. Waferly Company. USA. p. 260 – 274.
Lawrence, C. A. And S. S. Block. 1968. Desinfection, Sterilization and Preservation. Lea and Febiger. Philadelphia. P. 401 – 417.
Marcus, E. B., Daniel, R. D., Ming-Ju Huang, James, J. K., Emil, P., Nicholas, B.. 1991. Application Of Semiempirical Molecular Orbital Techniques To The Study Of Peroxidase Mediated Oxidation Of Phenols, Anilines, Sulfides And Thiobenzamides. 47 : 7525 – 7536.
Pelczar, M. J. and E. C. S. Chan. 1988. Dasar-dasar Mikrobiologi. Universitas Indonesia Press. hal. 99 – 105.
Rao, S. P. N. 1996. Zone of Inhibitions for Various Antibiotics-Kirby Bauer Disk Diffusion. (http://microrao.com). Diakses pada tanggal 23 Februari 2011. Pukul 11.05 WIB.
Retnawati, P. E. 2008. Pemberian Vaksin Polivalen dengan Chitosan dari Komponen Outer Membran Protein dan Lipopolisakarida Vibrio alginolyticus dan Vibrio anguillarum terhadap Sintasan Benih Kerapu Macan (Epinephelus fuscoguttatus). Tesis Universitas Airlangga. Surabaya.
Setyabudi, R. Dan H. S., Vincent. 1995. Farmakologi dan Terapi. Edisi 4. Farmakologi Kedokteran Universitas Indonesia. Jakarta.
Sugianti, B. 2005. Pemanfaatan Tumbuhan Obat Tradisional Dalam Pengendalian Penyakit Ikan. Makalah Pribadi Falsafah Sains Institut Pertanian Bogor. 3 : 1 – 37.
Trilaksani, W., Nurjanah, H.W Utama. 2006. Pemanfaatan Gelembung Renang Ikan Patin (Pangasius hypophthalmus) Sebagai Bahan Baku Isinglass. Buletin Teknologi Hasil Perikanan.
Wibowo, M. S. 2002. Penetapan Potensi Antibiotik Secara Mikrobiologi. ITB Bandung.
Zalizar, L. 2010. Efektifitas Salep Daun Sirih dan Meniran Terhadap Penurunan Jumlah Bakteri Pada Sapi Perah. Universitas Muhammadiyah Malang.