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Pengaruh Pemberian Vaksin Whole Cell Killed Virus Terhadap Sintasan Udang Vannamei (Lithopenaeus vannamei) Yang Diinfeksi Whitespot Baculovirus (WSBV)
[The Influence Of Whitespot Baculovirus (WSBV) Infection In Lithopenaeus vannamei By Giving Whole Cell Killed Virus Vaccine On Survival Rate.]
Corresponding Author(s) : Hari Suprapto
Jurnal Ilmiah Perikanan dan Kelautan, Vol. 2 No. 1 (2010): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Abstract
Abstract
Shrimp are susceptible to a wide variety of pathogens, one of each were viruses. Whitespot Baculovirus (WSBV) is one of virus attack in vannamei. Shrimp had been infected by WSBV will showed high mortality. One of Strategies for prophylaxis and control of WSBV is enhancement of disease resistant by using vaccines. Recently, quasi-immune response have been reported by which Penaeus japonicus surviving from WSBV infections possess a resistance against challenge WSBV (Venegas et al., 2000). This study was used Lithopenaeus vannamei that been vaccination with inactived-formaline WSBV virus cell or it called Whole Cell Killed Virus (WCKV) to know its responses to WSBV. The aim of this study was to know the effect Whole Cell Killed Virus vaccine to survival rate vannamei shrimp (Lithopenaeus vannamei) infected by Whitespot Baculovirus (WSBV). This study used descriptive study. The efficacy of vaccines made of inactivated WSSV with different dose. The dose are P1 (dose 0,01µg/ml each shrimp), P2 (dose 0,02 µg/ml each shrimp), P3 (dose 0,03 µg/ml each shrimp) dan P4 (non-vaccine (kontrol) injected by PBS). Primary parameter was survival rate (%). Secondary parameter was water quality which of temperature, pH, salinity and dissolved oxygen. The result showed that survival rate of L. Vannamei with different dose of WCKV vaccines was increases.
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- Astuti, S. M.2009. Penyakit Parasiter pada Udang. http:/www.tambak.org
- Azwar, S. 2004. Metode Penelitian. Pustaka Pelajar. Yogyakarta. 145 hal.
- Boyd, C. E.1989. Water Quality Management and Aeration in Shrimp Farming. Fisheries and Alied Aquaculture Departemential Series No 2. Alabama Agriculture Experiment Station. Auburn University. USA. 79 hal.
- Johnson, K. N., M. C. W. van Hulten and A. C. Barnes. 2008. Vaccination of Shrimps Against Viral Pathogens:Phenomenology and Underlying Mechanism. Vaccine 26:4885-4892.
- Liu, H., K. Soderhall and P. Jiravanichpaisal. 2009. Antiviral Immunity in Crustaceans. Journal Fish and Shellfish Immunology xxx (2009)1-10.
- Namikoshi A., J. L. Wu, T. Yamashita, T. Nishizawa, T.Nishioka, M. Arimoto and K. Muroga. 2003. Vaccinations Trials With Penaeus japonicas to Induce Resistance to White Spot Sindrome Virus. Aquaculture 229 (2004) 25-35.
- Nindarwi, D. N. 2006. Studi Perbandingan Penggunaan Whole Cell dan Extraceluller Product dari Vibrio alginolyticus sebagai Vaksin secara Oral Terhadap Sintasan Benih Ikan Kerapu Macan (Epinephelus fuscogutatus).Skripsi.Fakultas Perikanan d a n K e l a u t a n U n i v e r s i t a s Airlangga.Surabaya 56 hal.
- Nur'aini, Y. L., H. Bambang, S. Subyakto, dan T. Gemi. 2007. Active Surveilance of Infectious Myonecrosis Virus (IMNV) in Pond-Cultured White Shrimp (Lithopenaeus vannamei) in East Java and Bali. Jurnal Perikanan UGM. IX (1) : 25-31.
- Witteveldt, J. 2006. On the Vaccination of Shrimp Against White Spot Syndrome Virus. Thesis Wageningen University.129pp. Yeh, S.,
- Y. Chen, S. Hsieh, W. Cheng and C. Liu. 2008. mmune Response of White Shrimps, it. Litopenaeus vannamei, After A Concurrent Infection With White Spot Syndrome Virus a n d I n f e c t i o u s Hy p o d e rma l a n d Hematopoietic Necrosis Virus. Fish and
- Shellfish Immunology, 26 (2009):582-588.
- Zonneveld, N., E. A. Huisman and J. H. Boon. 2001. Prinsip-prinsip Budidaya Ikan. Gramedia Pustaka Utama. Jakarta. 317 hal.
References
Astuti, S. M.2009. Penyakit Parasiter pada Udang. http:/www.tambak.org
Azwar, S. 2004. Metode Penelitian. Pustaka Pelajar. Yogyakarta. 145 hal.
Boyd, C. E.1989. Water Quality Management and Aeration in Shrimp Farming. Fisheries and Alied Aquaculture Departemential Series No 2. Alabama Agriculture Experiment Station. Auburn University. USA. 79 hal.
Johnson, K. N., M. C. W. van Hulten and A. C. Barnes. 2008. Vaccination of Shrimps Against Viral Pathogens:Phenomenology and Underlying Mechanism. Vaccine 26:4885-4892.
Liu, H., K. Soderhall and P. Jiravanichpaisal. 2009. Antiviral Immunity in Crustaceans. Journal Fish and Shellfish Immunology xxx (2009)1-10.
Namikoshi A., J. L. Wu, T. Yamashita, T. Nishizawa, T.Nishioka, M. Arimoto and K. Muroga. 2003. Vaccinations Trials With Penaeus japonicas to Induce Resistance to White Spot Sindrome Virus. Aquaculture 229 (2004) 25-35.
Nindarwi, D. N. 2006. Studi Perbandingan Penggunaan Whole Cell dan Extraceluller Product dari Vibrio alginolyticus sebagai Vaksin secara Oral Terhadap Sintasan Benih Ikan Kerapu Macan (Epinephelus fuscogutatus).Skripsi.Fakultas Perikanan d a n K e l a u t a n U n i v e r s i t a s Airlangga.Surabaya 56 hal.
Nur'aini, Y. L., H. Bambang, S. Subyakto, dan T. Gemi. 2007. Active Surveilance of Infectious Myonecrosis Virus (IMNV) in Pond-Cultured White Shrimp (Lithopenaeus vannamei) in East Java and Bali. Jurnal Perikanan UGM. IX (1) : 25-31.
Witteveldt, J. 2006. On the Vaccination of Shrimp Against White Spot Syndrome Virus. Thesis Wageningen University.129pp. Yeh, S.,
Y. Chen, S. Hsieh, W. Cheng and C. Liu. 2008. mmune Response of White Shrimps, it. Litopenaeus vannamei, After A Concurrent Infection With White Spot Syndrome Virus a n d I n f e c t i o u s Hy p o d e rma l a n d Hematopoietic Necrosis Virus. Fish and
Shellfish Immunology, 26 (2009):582-588.
Zonneveld, N., E. A. Huisman and J. H. Boon. 2001. Prinsip-prinsip Budidaya Ikan. Gramedia Pustaka Utama. Jakarta. 317 hal.